Patents by Inventor Richard Paul Burlingame
Richard Paul Burlingame has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10415045Abstract: The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ?-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed.Type: GrantFiled: September 9, 2015Date of Patent: September 17, 2019Assignee: DANISCO US INCInventors: Peter J. Punt, Richard Paul Burlingame, Christine M. Pynnonen, Phillip T. Olson, Jan Wery, Johannes Heinrich Visser, Mark A. Emalfarb, Jacob Visser, Jan Cornelis Verdoes
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Publication number: 20150376629Abstract: The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ?-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed.Type: ApplicationFiled: September 9, 2015Publication date: December 31, 2015Inventors: Peter J. PUNT, Richard Paul BURLINGAME, Christine M. PYNNONEN, Phillip T. OLSON, Jan WERY, Johannes Heinrich VISSER, Mark A. EMALFARB, Jacob VISSER, Jan Cornelis VERDOES, Jacoba VERDOES
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Patent number: 9175296Abstract: The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ?-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed.Type: GrantFiled: March 16, 2010Date of Patent: November 3, 2015Assignee: DYADIC NEDERLAND B.V.Inventors: Peter J. Punt, Richard Paul Burlingame, Christine M. Pynnonen, Phillip T. Olson, Jan Wery, Johannes Heinrich Visser, Mark A. Emalfarb, Jacob Visser, Jan Cornelis Verdoes, Jacoba Verdoes
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Patent number: 8871493Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.Type: GrantFiled: September 17, 2012Date of Patent: October 28, 2014Assignee: Dyadic International (USA), Inc.Inventors: Mark Aaron Emalfarb, Richard Paul Burlingame, Philip Terry Olson, Arkady Panteleimonovich Sinitsyn, Martine Parriche, Jean Christophe Bousson, Christine Marie Pynnonen, Peter Jan Punt, Cornelia Marie Johanna Van Zeijl
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Publication number: 20140127788Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.Type: ApplicationFiled: September 17, 2012Publication date: May 8, 2014Inventors: Mark Aaron Emalfarb, Richard Paul Burlingame, Philip Terry Olson, Arkady Panteleimonovich Sinitsyn, Martine Parriche, Jean Christophe Bousson, Christine Marie Pynnonen, Peter Jan Punt, Cornelia Maria Johanna Van Zeijl
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Patent number: 8268585Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.Type: GrantFiled: March 13, 2008Date of Patent: September 18, 2012Assignee: Dyadic International (USA), Inc.Inventors: Mark Aaron Emalfarb, Richard Paul Burlingame, Philip Terry Olson, Arkady Panteleimonovich Sinitsyn, Martine Parriche, Jean Christophe Bousson, Christine Marie Pynnonen, Peter Jan Punt, Cornelia Maria Johanna Van Zeijl
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Publication number: 20120107856Abstract: The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ?-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed.Type: ApplicationFiled: March 16, 2010Publication date: May 3, 2012Inventors: Peter J. Punt, Richard Paul Burlingame, Christine M. Pynnonen, Phillip T. Olson, Jan Wery, Johannes Heinrich Visser, Mark A. Emalfarb, Jacob Visser, Jan Cormelis Verdoes, Jacoba Verdoes
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Publication number: 20080194005Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.Type: ApplicationFiled: March 13, 2008Publication date: August 14, 2008Inventors: Mark Aaron EMALFARB, Richard Paul Burlingame, Philip Terry Olson, Arkady Panteleimonovich Sinitsyn, Martine Parriche, Jean Christophe Bousson, Christine Marie Pynnonen, Peter Jan Punt, Cornelia Maria Johanna Van Zeijl
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Patent number: 7399627Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.Type: GrantFiled: March 21, 2003Date of Patent: July 15, 2008Assignee: Dyadic International (USA), Inc.Inventors: Mark Aaron Emalfarb, Richard Paul Burlingame, Philip Terry Olson, Arkady Panteleimonovich Sinitsyn, Martine Parriche, Jean Christophe Bousson, Christine Marie Pynnonen, Peter Jan Punt, Cornelia Marie Johanna Van Zeijl
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Publication number: 20040002136Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.Type: ApplicationFiled: March 21, 2003Publication date: January 1, 2004Inventors: Mark Aaron Emalfarb, Richard Paul Burlingame, Philip Terry Olson, Arkady Panteleimonovich Sinitsyn, Martine Parriche, Jean Christophe Bousson, Christine Marie Pynnonen, Peter Jan Punt, Cornelia Maria Johanna Van Zeijl
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Patent number: 6573086Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.Type: GrantFiled: April 13, 2000Date of Patent: June 3, 2003Assignee: Dyadic International, Inc.Inventors: Mark Aaron Emalfrab, Richard Paul Burlingame, Philip Terry Olson, Arkady Panteleimonovich Sinitsyn, Martine Parriche, Jean Christophe Bousson, Christine Marie Pynnonen, Peter Jan Punt, Cornelia Marie Johanna Van Zeijl