Abstract: Embodiments of the present invention provide for novel compositions and methods for making and using a thermally stable broad spectrum human papilloma virus (HPV) vaccine or immunogenic formulation. Certain embodiments concern lyophilizing HPV formulations in the presence or absence of adjuvants. Other embodiments concern lyophilizing HPV capsomere vaccines and other immunogenic agents to increase stability or reduce degradation of HPV peptides to prolong storage, delivery and use. In yet other embodiments, a single immunogenic formulation can include a thermally stable composition of a broad-spectrum HPV immunogenic composition against multiple HPV types. In some embodiments, a stabilizing formulation can include RG1 HPV16VLP antigens in a hypertonic mixture of a disaccharide and a volatile buffer.

Abstract: The present invention provides methods of mucosal tissue administration of therapeutic HPV vaccines, in a prime-boost regimen, which generated antigen-specific CD8+ T cell-mediated immune responses and the expression of tissue-resident memory T cell (Trm) markers on the CD8+ T cells. In some embodiments, the inventive methods employed pNGVL4a-sig/E7(detox)/HSP70 DNA vaccine and TA-HPV in a prime-boost regimen which shows vaccination in the infected mucosal tissues, including those in the cervicovaginal tract, elicited potent antitumor effects and a more effective local immune response in the tissues and regional lymph node, when compared to intramuscular vaccination. Furthermore, targeting the induction of Trm-mediated immune responses can serve as an ideal methodology especially for therapeutic HPV vaccines.

Abstract: Embodiments of the invention are directed to methods and compositions of multitype HPV polypeptides.

Abstract: Methods for treating or preventing recurrence of hyper proliferating diseases, e.g., cancer and persistent viral infections, are described. A method may comprise priming a mammal by administering to the mammal an effective amount of a nucleic acid composition encoding an antigen or a biologically active homolog thereof and boosting the mammal by administering to the mammal an effective amount of an oncolytic virus comprising a nucleic acid encoding the antigen or the biologically active homolog thereof.

Abstract: Methods for delivering naked DNA vaccines to enhance immune responses, by improving transfection efficiency without safety concerns associated with live viral vectors, are described. A method may comprise administering to a mammalian subject an effective amount of a papillomavirus pseudovirion, wherein the papillomavirus pseudovirion comprises at least one papillomavirus capsid protein encapsidating a naked DNA vaccine, wherein the naked DNA vaccine comprises a first nucleic acid encoding at least one antigen, thereby enhancing the antigen specific immune response relative to administration of the naked DNA vaccine.

Abstract: Embodiments of the invention are directed to methods and compositions of multitype HPV polypeptides.

Abstract: Human papillomavirus (HPV) infection is the etiological factor for cervical cancer. Provided are HPV vaccines that generate a humoral immune response to prevent new infection, as well as cell-mediated immunotherapy to eliminate established infection or HPV-related disease. HPV vaccines include nucleic acid sequences encoding HPV16 early proteins E6 and E7. Additional nucleic acid sequences in the vaccines include sequences encoding calreticulin and/or the HPV16 late protein L2. Methods using these vaccines are provided that result in therapeutic effects.

Abstract: The present invention is based on the discovery of autoantibodies in cancer patients specific for a number of antigens that are normally intracellular, including homeobox protein HOXA7, homeobox protein HOXB7, ADP-ribosylation factor 1 (Arf-1), ATP-dependent iron transporter ABC-7, and a novel protein encoded by a EcoRI/XhoI fragment of bacteriophage ? clone 44B.1 deposited under ATCC accession No. ______. The presence of these autoantibodies can be correlated with neoplastic processes in patients, and therefore detection of autoantibodies (or detection of expression of the antigens by other means) can be used as a component of a cancer screening program. The present invention provides such screening assays. In addition, the studies leading to the identification of the predictive autoantigens have also succeeded in identifying a hitherto unknown antigen that is disclosed herein.