Patents by Inventor Richard S. Handley
Richard S. Handley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20130084652Abstract: Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. Non-specific signal generated by singlet oxygen not in proximity is reduced or suppressed using a singlet oxygen quencher (SOQ). The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Use of Noise Modulation Agents significantly improves signal-to-noise ratios and assay sensitivity. Compositions and kits are also disclosed.Type: ApplicationFiled: May 13, 2011Publication date: April 4, 2013Applicant: BECKMAN COULTER, INC.Inventors: Nir Shapir, Michael Salvati, Jeff Todtleben, Hashem Akhavan-Tafti, Richard S. Handley
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Patent number: 8137990Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O?is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.Type: GrantFiled: August 14, 2009Date of Patent: March 20, 2012Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Patent number: 7704752Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O? is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.Type: GrantFiled: June 23, 2008Date of Patent: April 27, 2010Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Publication number: 20100055800Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O?is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.Type: ApplicationFiled: August 14, 2009Publication date: March 4, 2010Applicant: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Publication number: 20080261318Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O?is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.Type: ApplicationFiled: June 23, 2008Publication date: October 23, 2008Applicant: LUMIGEN, INC.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Patent number: 7390670Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig—OH or Sig—O? is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.Type: GrantFiled: February 20, 2003Date of Patent: June 24, 2008Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Publication number: 20070190526Abstract: Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.Type: ApplicationFiled: February 15, 2007Publication date: August 16, 2007Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Robert A. Eickholt, Michael E. Mazelis, Wenhuas Xie, Richard S. Handley, Monica A. Bray, Michelle L. Mastronardi, Elizabeth A. O'Conner, Sarada Siripurapu
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Patent number: 6919463Abstract: Compounds useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is an aromatic or heteroaromatic ring group, B is a boron atom, and R5 and R6 are independently selected from hydrogen and lower alkyl groups and can be joined together as a straight or branched alkylene chain forming a five or six-membered ring. A detectable product compound of the formula Sig-OH Is produced and detected by measuring color, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The compounds can be used for detection in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.Type: GrantFiled: June 20, 2003Date of Patent: July 19, 2005Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Publication number: 20040171098Abstract: Compounds useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: 1Type: ApplicationFiled: June 20, 2003Publication date: September 2, 2004Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Publication number: 20040166539Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: 1Type: ApplicationFiled: February 20, 2003Publication date: August 26, 2004Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Patent number: 6774249Abstract: Improved polymer-immobilized photosensitizer are disclosed as well as methods of preparing and using them. The polymer-immobilized photosensitizers comprise a cross-linked polymer backbone, a plurality of cationic ammonium or phosphonium groups covalently bound to the polymer backbone and an immobilized photosensitizer. The average total number of carbon atoms in the ammonium or phosphonium group is at least four and preferably at least 12. The photosensitizer can be either covalently or ionically bound to the polymer. Polymer-supported photosensitizers of the invention are unexpectedly superior in catalyzing the photosensitized oxidation of compounds containing carbon-carbon double bonds.Type: GrantFiled: September 27, 2001Date of Patent: August 10, 2004Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Richard S. Handley, Mark D. Sandison, Randall K. Larkin
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Publication number: 20030095916Abstract: Improved polymer-immobilized photosensitizer are disclosed as well as methods of preparing and using them. The polymer-immobilized photosensitizers comprise a cross-linked polymer backbone, a plurality of cationic ammonium or phosphonium groups covalently bound to the polymer backbone and an immobilized photosensitizer. The average total number of carbon atoms in the ammonium or phosphonium group is at least four and preferably at least 12. The photosensitizer can be either covalently or ionically bound to the polymer. Polymer-supported photosensitizers of the invention are unexpectedly superior in catalyzing the photosensitized oxidation of compounds containing carbon-carbon double bonds.Type: ApplicationFiled: September 27, 2001Publication date: May 22, 2003Inventors: Hashem Akhavan-Tafti, Richards S. Handley, Mark D. Sandison, Randall K. Larkin
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Patent number: 6545102Abstract: Improved polymer-immobilized photosensitizer are disclosed as well as methods of preparing and using them. The polymer-immobilized photosensitizers comprise a cross-linked polymer backbone, a plurality of cationic ammonium or phosphonium groups covalently bound to the polymer backbone and an immobilized photosensitizer. The average total number of carbon atoms in the ammonium or phosphonium group is at least four and preferably at least 12. The photosensitizer can be either covalently or ionically bound to the polymer. Polymer-supported photosensitizers of the invention are unexpectedly superior in catalyzing the photosensitized oxidation of compounds containing carbon-carbon double bonds.Type: GrantFiled: September 27, 2001Date of Patent: April 8, 2003Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Richard S. Handley, Mark D. Sandison, Randall K. Larkin
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Patent number: 6410732Abstract: Synthetic processes and intermediates are disclosed for the preparation of N-arylacridancarboxylic acid derivatives. The derivatives are esters, thioesters, amides and sulfonimides. A key feature of the processes is the preparation of N-aryl substituted intermediates by formation of a bond between the nitrogen atom of the acridan ring and a carbon atom of another aromatic or heteroaromatic ring compound. The arylation reaction is catalyzed by a palladium catalyst. The N-arylacridancarboxylic acid derivatives are useful in methods for producing light and in assays for peroxidase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.Type: GrantFiled: January 25, 2001Date of Patent: June 25, 2002Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Richard S. Handley
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Publication number: 20010031869Abstract: Synthetic processes and intermediates are disclosed for the preparation of N-arylacridancarboxylic acid derivatives. The derivatives are esters, thioesters, amides and sulfonimides. A key feature of the processes is the preparation of N-aryl substituted intermendiates by formation of a bond between the nitrogen atom of the acridan ring and a carbon atom of another aromatic or heteroaromatic ring compound. The arylation reaction is catalyzed by a palladium catalyst. The N-arylacridancarboxylic acid derivatives are useful in methods for producing light and in assays for peroxidase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.Type: ApplicationFiled: January 25, 2001Publication date: October 18, 2001Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Richard S. Handley
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Patent number: 5601977Abstract: A method and test kit using a hydroxyaryl cyclic diacylhydrazide, a peroxide and a peroxidase enzyme on a blotting membrane for detecting DNA, RNA or proteins (polypeptides) is described. The method and kit provides enhanced chemiluminescence in an assay because of the use of the membrane. The method and test kit is particularly useful in Western, Southern and Northern blotting type assays and DNA sequencing.Type: GrantFiled: December 23, 1992Date of Patent: February 11, 1997Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Richard S. Handley