Abstract: There is disclosed fluid distribution junctions and methods of assembly therefor. Flexible conduits connect to the junctions, and a consumable subsystem of the junction, conduits and receptacle caps or other connectors may be pre-assembled for ease of use. A subassembly is formed by coupling a plurality of flexible tubular conduits to a plurality of fluid connectors of a fluid junction, the fluid junction having an inner fluid plenum chamber leading to the fluid connectors. Two shells are sandwiched on opposite sides of the subassembly, the shells having mating concave receiving surfaces that together conform around each of the fluid connectors and clamp the tubular conduits onto the circular beads. Juxtaposed joint surfaces on each pair of mating concave receiving surfaces are bonded together such as with sonic welding to make the fluid distribution junction assembly.

Abstract: A mixing system for use in a process bottle for mixing its contents, the mixing system including a low volume magnetically-driven mixer mounted at the bottom of the bottle. The mixer may have vanes and lower grooves, or no vanes and grooves on both upper and lower faces. The mixer has a “microsized” three-dimensional solid inanimate body to enable insertion through relatively small mouth openings at the top of conventional reactor bottles. Methods of assembly are also disclosed which involve passing the microsized mixer through an open mouth of a process bottle and coupling the mixer with a bearing assembly at the floor of the bottle. The bearing assembly includes fixtures sealed around a hole in the floor of the bottle.

Abstract: A process heat exchange rod for cooling or heating liquids in a process vessel. The rod may have a linear form and extend downward through an upper wall of the process vessel into proximity with the lower floor. The rod internally defines a circulatory flow path for the heat exchange medium, including an outer jacket and a flow diverter having a central through bore and external helical flutes. Heat exchange medium travels down through the central through bore and then back up through helical grooves formed between the flow diverter and the outer jacket, or vice versa. Accurate heating or cooling of the process fluid is attained by modification of the configuration of the heat exchange rod as well as the flow rate and temperature of the heat exchange medium. The components may be injection molded of a polymer, often transparent, having a high heat transfer coefficient.

Abstract: A mixing system for use in a process bottle for mixing its contents, the mixing system including a low volume magnetically-driven mixer mounted at the bottom of the bottle. The mixer may have vanes and lower grooves, or no vanes and grooves on both upper and lower faces. The mixer has a “microsized” three-dimensional solid inanimate body to enable insertion through relatively small mouth openings at the top of conventional reactor bottles. Methods of assembly are also disclosed which involve passing the microsized mixer through an open mouth of a process bottle and coupling the mixer with a bearing assembly at the floor of the bottle. The bearing assembly includes fixtures sealed around a hole in the floor of the bottle.

Abstract: A process heat exchange rod for cooling or heating liquids in a process vessel. The rod may have a linear form and extend downward through an upper wall of the process vessel into proximity with the lower floor. The rod internally defines a circulatory flow path for the heat exchange medium, including an outer jacket and a flow diverter having a central through bore and external helical flutes. Heat exchange medium travels down through the central through bore and then back up through helical grooves formed between the flow diverter and the outer jacket, or vice versa. Accurate heating or cooling of the process fluid is attained by modification of the configuration of the heat exchange rod as well as the flow rate and temperature of the heat exchange medium. The components may be injection molded of a polymer, often transparent, having a high heat transfer coefficient.

Abstract: A mixing system for use in a process bottle for mixing its contents, the mixing system including a low volume magnetically-driven mixer mounted at the bottom of the bottle. The mixer may have vanes and lower grooves, or no vanes and grooves on both upper and lower faces. The mixer has a “microsized” three-dimensional solid inanimate body to enable insertion through relatively small mouth openings at the top of conventional reactor bottles. Methods of assembly are also disclosed which involve passing the microsized mixer through an open mouth of a process bottle and coupling the mixer with a bearing assembly at the floor of the bottle. The bearing assembly includes fixtures sealed around a hole in the floor of the bottle.

Abstract: A process heat exchange rod for cooling or heating liquids in a process vessel. The rod may have a linear form and extend downward through an upper wall of the process vessel into proximity with the lower floor. The rod internally defines a circulatory flow path for the heat exchange medium, including an outer jacket and a flow diverter having a central through bore and external helical flutes. Heat exchange medium travels down through the central through bore and then back up through helical grooves formed between the flow diverter and the outer jacket, or vice versa. Accurate heating or cooling of the process fluid is attained by modification of the configuration of the heat exchange rod as well as the flow rate and temperature of the heat exchange medium. The components may be injection molded of a polymer, often transparent, having a high heat transfer coefficient.

Abstract: A closed fluid receiving and sampling container that enables transfer of valuable reaction liquid to a receptacle without risking loss of sterility. The sampling container has a dip tube subassembly with a shorter inlet tube bent towards the wall of the receptacle to prevent or reduce foaming, and a longer outlet tube used to drain the waste liquid once the magnetic beads are trapped by the magnet. The dip tube subassembly is injection molded in one piece and provides a sealed cap also with a vent tube therethrough to enable filling and draining the receptacle without removing the cap, thus keeping the process aseptic. The sampling container is especially useful in the context of magnetic bead separation processes.

Abstract: A process heat exchange rod for cooling or heating liquids in a process vessel. The rod may have a linear form and extend downward through an upper wall of the process vessel into proximity with the lower floor. The rod internally defines a circulatory flow path for the heat exchange medium, including an outer jacket and a flow diverter having a central through bore and external helical flutes. Heat exchange medium travels down through the central through bore and then back up through helical grooves formed between the flow diverter and the outer jacket, or vice versa. Accurate heating or cooling of the process fluid is attained by modification of the configuration of the heat exchange rod as well as the flow rate and temperature of the heat exchange medium. The components may be injection molded of a polymer, often transparent, having a high heat transfer coefficient.

Abstract: A closed fluid receiving and sampling container that enables transfer of valuable reaction liquid to a receptacle without risking loss of sterility. The sampling container has a dip tube subassembly with a shorter inlet tube bent towards the wall of the receptacle to prevent or reduce foaming, and a longer outlet tube used to drain the waste liquid once the magnetic beads are trapped by the magnet. The dip tube subassembly is injection molded in one piece and provides a sealed lid also with a vent tube therethrough to enable filling and draining the receptacle without removing the lid, thus keeping the process aseptic. The sampling container is especially useful in the context of magnetic bead separation processes.

Abstract: A clamping device, having a plurality of clamp segments, each clamp segment, having an arcuate base, each arcuate base having an interior side and an exterior side, a first pair of sidewalls extruding radially inward from the interior side of the arcuate base, thereby defining an interior channel, wherein the sidewalls are conformable to a fitting inside the interior channel, a second pair of sidewalls extruding radially outward from the exterior side of the arcuate base, thereby defining an exterior channel, wherein each clamp segment is interconnected with at least one other clamp segment by a hinge; and a cable tie to keep the plurality of clamp segments in a closed configuration.

Abstract: A clamping device, constructed as a single piece, yet comprising a plurality of clamp segments, each clamp segment, comprising an arcuate base having an interior side and an exterior side, a first pair of sidewalls extruding radially inward from the interior side of the arcuate base, thereby defining an interior channel, wherein the sidewalls are conformable to a fitting inside the interior channel, a second pair of sidewalls extruding radially outward from the exterior side of the arcuate base, thereby defining an exterior channel, wherein each clamp segment is interconnected with at least one other clamp segment by a hinge; and a cable tie comprising a first end and a second end fastenable to the first end, wherein the cable tie is housed inside the exterior channel of each clamp segment and passed through a first cable guard at the first free end and a second cable guard at the second free end.