Abstract: The present disclosure provides a method for analyzing nucleic acid sequences. The method can comprise determining, by a computer system, a base trace by trimming a Sanger sequencing trace of a plurality of nucleic acid molecules from a sample based on a first target sequence and a second target sequence. Each of the first and second target sequences can be in the plurality of nucleic acid molecules or can be in the complement of sequence of the plurality of nucleic acid molecules.

Abstract: Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: Described herein are methods for generating and using variant panels of clonally expanded cells containing a plurality of introduced genetic variants. These clonally expanded cells can be partitioned such that each individual partition contains a single genetic variant, allowing for the assessment of the outcome of each variant without the confounding effect of background genetic variation. Further, panels of such variants can be used to evaluate nucleic acid repair strategies. Genetic variation can be introduced through the use of genome editing tools, such as CRISPR/Cas.

Abstract: An illustrative embodiment of a grow board may include an outer member that may be engaged with an inner member such that a grow media may be positioned therebetween, secured, and/or tightened by the engagement between the inner and outer members. The outer member may have one or more slots formed therein to allow a plant to grow from the grow media through the slots, wherein the root of the plant may be positioned adjacent the inner member. The inner member may be configured with risers along the periphery and interior to provide a sufficient gap between stacked grow boards to allow for improved penetration of light and air into the seeded and stacked grow boards to aid in seed germination.

Abstract: The present disclosure provides systems and methods for gaze tracking. The methods for gaze tracking may comprise (a) collecting video data of a subject's face using a device, and (b) processing the video data to track the subject's gaze. The video data may comprise a plurality of images containing depth information. The methods for gaze tracking may track the gaze of the subject without requiring any prior or subsequent calibration of the subject's gaze to a predefined calibration point in space having a known location. The systems for gaze tracking may be configured to implement the methods for gaze tracking.

Abstract: The present disclosure provides systems and methods for gaze tracking. The methods for gaze tracking may comprise (a) collecting video data of a subject's face using a device, and (b) processing the video data to track the subject's gaze. The video data may comprise a plurality of images containing depth information. The methods for gaze tracking may track the gaze of the subject without requiring any prior or subsequent calibration of the subject's gaze to a predefined calibration point in space having a known location. The systems for gaze tracking may be configured to implement the methods for gaze tracking.

Abstract: An illustrative embodiment of a grow board may include an outer member that may be engaged with an inner member such that a grow media may be positioned therebetween, secured, and/or tightened by the engagement between the inner and outer members. The outer member may have one or more slots formed therein to allow a plant to grow from the grow media through the slots, wherein the root of the plant may be positioned adjacent the inner member. The inner member may be configured with risers along the periphery and interior to provide a sufficient gap between stacked grow boards to allow for improved penetration of light and air into the seeded and stacked grow boards to aid in seed germination.

Abstract: The present disclosure provides a method for analyzing nucleic acid sequences. The method can comprise determining, by a computer system, a base trace by trimming a Sanger sequencing trace of a plurality of nucleic acid molecules from a sample based on a first target sequence and a second target sequence. Each of the first and second target sequences can be in the plurality of nucleic acid molecules or can be in the complement of sequence of the plurality of nucleic acid molecules.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: An illustrative embodiment of a grow board may include an outer member that may be engaged with an inner member such that a grow media may be positioned therebetween, secured, and/or tightened by the engagement between the inner and outer members. The outer member may have one or more slots formed therein to allow a plant to grow from the grow media through the slots, wherein the root of the plant may be positioned adjacent the inner member. The inner member may be configured with risers along the periphery and interior to provide a sufficient gap between stacked grow boards to allow for improved penetration of light and air into the seeded and stacked grow boards to aid in seed germination.

Abstract: An illustrative embodiment of a grow board may include an outer member that may be engaged with an inner member such that a grow media may be positioned therebetween, secured, and/or tightened by the engagement between the inner and outer members. The outer member may have one or more slots formed therein to allow a plant to grow from the grow media through the slots, wherein the root of the plant may be positioned adjacent the inner member. The inner member may be configured with risers along the periphery and interior to provide a sufficient gap between stacked grow boards to allow for improved penetration of light and air into the seeded and stacked grow boards to aid in seed germination.

Abstract: Liquid fractionation systems utilizing a novel low pressure liquid delivery system generated by centrifugal force utilizing a rotating cylinder device. The rotating cylinder device distributes liquid solution to the roots of plants by use of centrifugal force, thereby eliminating the need for a high-pressure pump and nozzles. The geometrical shape of the enclosed root growth chamber is such that it allows for fractionated droplets to ricochet in multiple random directions thus completely surrounding the plant roots in 360° in any plane. The invention also provides aeroponics apparati utilizing the low-pressure liquid delivery system, methods for fractionating liquid, methods for delivering the fractionated liquid to plants, and methods for growing plants and germinating seeds.