Patents by Inventor Richard Torres
Richard Torres has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240011977Abstract: The present invention relates to systems and methods for tissue processing and imaging including simultaneous dehydration and staining with fluorescent nuclear and protein dyes followed by optical sectioning microscopy.Type: ApplicationFiled: July 5, 2023Publication date: January 11, 2024Inventors: Michael Levene, Richard Torres
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Publication number: 20230204474Abstract: The present invention relates to systems and methods for tissue processing and analysis. Tissue chambers are configured to allow single-container chemical processing, imaging, and wax embedding of tissue samples in a single container without manipulation between steps. Tissue chambers with features to support the tissue sample and allow fluid flow between the tissue sample and the tissue chamber surface are disclosed. The features may be index matched to sample structures of interest or dissolvable in clearing solution to allow for in-chamber imaging with minimal distortion. Specialized tissue processing and wax removal apparatuses are also disclosed including for use with tissue chambers having frangible portions to permit ease of wax removal.Type: ApplicationFiled: January 13, 2023Publication date: June 29, 2023Inventors: Michael Levene, Richard Torres
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Patent number: 11573179Abstract: The present invention relates to systems and methods for tissue processing and imaging including a counterintuitive inverse relationship between protein dye concentration and tissue sample protein content. Varying dye concentration in such a manner affords higher quality fluorescent imaging at depth in tissue when using optical sectioning microscopy or second harmonic generation (SHG) analysis. Methods of the invention thereby provide more usable histopathology images while reducing waste and reagent costs and are of particular utility in combination assays that include simultaneous protein and nuclear staining and SHG analysis.Type: GrantFiled: August 24, 2020Date of Patent: February 7, 2023Assignee: APPLIKATE TECHNOLOGIES LLCInventors: Richard Torres, Michael Levene
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Patent number: 11555765Abstract: The present invention relates to systems and methods for tissue processing and analysis. Tissue chambers are configured to allow single-container chemical processing, imaging, and wax embedding of tissue samples in a single container without manipulation between steps. Tissue chambers with features to support the tissue sample and allow fluid flow between the tissue sample and the tissue chamber surface are disclosed. The features may be index matched to sample structures of interest or dissolvable in clearing solution to allow for in-chamber imaging with minimal distortion. Specialized tissue processing and wax removal apparatuses are also disclosed including for use with tissue chambers having frangible portions to permit ease of wax removal.Type: GrantFiled: January 6, 2020Date of Patent: January 17, 2023Assignee: APPLIKATE TECHNOLOGIES LLCInventors: Michael Levene, Richard Torres
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Patent number: 11518804Abstract: Methods are disclosed for treating osteoarthritis in a human subject in need thereof, comprising administering to the subject a therapeutically effective amount of an anti-human NGF antibody, or antigen-binding fragment thereof, wherein at least one symptom associated with osteoarthritis is prevented, ameliorated or improved.Type: GrantFiled: July 10, 2020Date of Patent: December 6, 2022Assignee: REGENERON PHARMACEUTICALS, INC.Inventors: Lynn MacDonald, Richard Torres, Marc R. Morra, Joel H. Martin, Joel C. Reinhardt, Paul Tiseo
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Publication number: 20210072156Abstract: The present invention relates to systems and methods for tissue processing and imaging including a counterintuitive inverse relationship between protein dye concentration and tissue sample protein content. Varying dye concentration in such a manner affords higher quality fluorescent imaging at depth in tissue when using optical sectioning microscopy or second harmonic generation (SHG) analysis. Methods of the invention thereby provide more usable histopathology images while reducing waste and reagent costs and are of particular utility in combination assays that include simultaneous protein and nuclear staining and SHG analysis.Type: ApplicationFiled: August 24, 2020Publication date: March 11, 2021Inventors: Richard Torres, Michael Levene
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Publication number: 20200371033Abstract: The invention provides improved systems and methods for multiphoton microscopy including pixel clocking techniques for minimizing pixel integration time and providing consistent signal intensity with maximized imaging speeds. Various systems and method are described for optimizing laser repetition rate based on dye lifetime, combining polygonal mirror scanning and stage translation, using the laser pulse signal to time pixel collection, and minimizing laser pulses and dye usage based on signal to background ratios.Type: ApplicationFiled: June 8, 2020Publication date: November 26, 2020Inventors: Michael Levene, Richard Torres
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Publication number: 20200348216Abstract: The present invention relates to systems and methods for tissue processing and analysis. Tissue chambers are configured to allow single-container chemical processing, imaging, and wax embedding of tissue samples in a single container without manipulation between steps. Tissue chambers with features to support the tissue sample and allow fluid flow between the tissue sample and the tissue chamber surface are disclosed. The features may be index matched to sample structures of interest or dissolvable in clearing solution to allow for in-chamber imaging with minimal distortion. Specialized tissue processing and wax removal apparatuses are also disclosed including for use with tissue chambers having frangible portions to permit ease of wax removal.Type: ApplicationFiled: January 6, 2020Publication date: November 5, 2020Inventors: Michael Levene, Richard Torres
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Publication number: 20200339672Abstract: Methods are disclosed for treating osteoarthritis in a human subject in need thereof, comprising administering to the subject a therapeutically effective amount of an anti-human NGF antibody, or antigen-binding fragment thereof, wherein at least one symptom associated with osteoarthritis is prevented, ameliorated or improved.Type: ApplicationFiled: July 10, 2020Publication date: October 29, 2020Applicant: REGENERON PHARMACEUTICALS, INC.Inventors: Lynn MACDONALD, Richard TORRES, Marc R. MORRA, Joel H. MARTIN, Joel C. REINHARDT, Paul TISEO
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Patent number: 10745471Abstract: Methods are disclosed for treating osteoarthritis in a human subject in need thereof, comprising administering to the subject a therapeutically effective amount of an anti-human NGF antibody, or antigen-binding fragment thereof, wherein at least one symptom associated with osteoarthritis is prevented, ameliorated or improved.Type: GrantFiled: February 21, 2019Date of Patent: August 18, 2020Assignee: REGENERON PHARMACEUTICALS, INC.Inventors: Lynn MacDonald, Richard Torres, Marc R. Morra, Joel H. Martin, Joel C. Reinhardt, Paul Tiseo
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Patent number: 10677730Abstract: The invention provides improved systems and methods for multiphoton microscopy including pixel clocking techniques for minimizing pixel integration time and providing consistent signal intensity with maximized imaging speeds. Various systems and method are described for optimizing laser repetition rate based on dye lifetime, combining polygonal mirror scanning and stage translation, using the laser pulse signal to time pixel collection, and minimizing laser pulses and dye usage based on signal to background ratios.Type: GrantFiled: July 26, 2019Date of Patent: June 9, 2020Assignee: APLLIKATE TECHNOLOGIES LLCInventors: Michael Levene, Richard Torres
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Patent number: 10591392Abstract: A biopsy-sized tissue sample is stained for quick imaging. A significant amount of permeation enhancer is included in a mixed solution of permeant enhancer, fixative or dehydrant, and one or two fluorescent dyes to simultaneously dehydrate and dye the tissue sample. The permeation enhancer, e.g., 10% to 50% in the mixed solution, achieves an image of dyed tissue in the contacted tissue sample at a depth of at least 200 um within no more than 1.5 hours. One of the fluorescent dyes is a fluorescent nuclear dye such as DAPI, SYTOX green, acridine orange, propidium iodide, or a Hoechst dye. The other fluorescent dye is a fluorescent protein dye such as eosin or rhodamine B. The tissue sample is cleared with a clearing agent having a refractive index of at least 1.4[R2], e.g., using BABB. The mixed solution may further include Chloroform or other morphology preservative.Type: GrantFiled: July 3, 2014Date of Patent: March 17, 2020Assignee: APPLIKATE TECHNOLOGIES LLCInventors: Richard Torres, Michael Levene
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Patent number: 10527528Abstract: The present invention relates to systems and methods for tissue processing and analysis. Tissue chambers are configured to allow single-container chemical processing, imaging, and wax embedding of tissue samples in a single container without manipulation between steps. Tissue chambers with features to support the tissue sample and allow fluid flow between the tissue sample and the tissue chamber surface are disclosed. The features may be index matched to sample structures of interest or dissolvable in clearing solution to allow for in-chamber imaging with minimal distortion. Specialized tissue processing and wax removal apparatuses are also disclosed including for use with tissue chambers having frangible portions to permit ease of wax removal.Type: GrantFiled: October 18, 2018Date of Patent: January 7, 2020Assignee: APPLIKATE TECHNOLOGIES LLCInventors: Michael Levene, Richard Torres
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Publication number: 20190360901Abstract: The present invention relates to systems and methods for tissue processing and analysis. Tissue chambers are configured to allow single-container chemical processing, imaging, and wax embedding of tissue samples in a single container without manipulation between steps. Tissue chambers with features to support the tissue sample and allow fluid flow between the tissue sample and the tissue chamber surface are disclosed. The features may be index matched to sample structures of interest or dissolvable in clearing solution to allow for in-chamber imaging with minimal distortion. Specialized tissue processing and wax removal apparatuses are also disclosed including for use with tissue chambers having frangible portions to permit ease of wax removal.Type: ApplicationFiled: October 18, 2018Publication date: November 28, 2019Inventors: Michael Levene, Richard Torres
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Publication number: 20190211090Abstract: Methods are disclosed for treating osteoarthritis in a human subject in need thereof, comprising administering to the subject a therapeutically effective amount of an anti-human NGF antibody, or antigen-binding fragment thereof, wherein at least one symptom associated with osteoarthritis is prevented, ameliorated or improved.Type: ApplicationFiled: February 21, 2019Publication date: July 11, 2019Applicant: REGENERON PHARMACEUTICALS, INC.Inventors: Lynn MACDONALD, Richard TORRES, Marc R. MORRA, Joel H. MARTIN, Joel C. REINHARDT, Paul TISEO
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Patent number: 10266588Abstract: Methods are disclosed for treating osteoarthritis in a human subject in need thereof, comprising administering to the subject a therapeutically effective amount of an anti-human NGF antibody, or antigen-binding fragment thereof, wherein at least one symptom associated with osteoarthritis is prevented, ameliorated or improved.Type: GrantFiled: May 2, 2016Date of Patent: April 23, 2019Assignee: REGENERON PHARMACEUTICALS, INC.Inventors: Lynn MacDonald, Richard Torres, Marc R. Morra, Joel H. Martin, Joel C. Reinhardt, Paul Tiseo
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Publication number: 20160237148Abstract: Methods are disclosed for treating osteoarthritis in a human subject in need thereof, comprising administering to the subject a therapeutically effective amount of an anti-human NGF antibody, or antigen-binding fragment thereof, wherein at least one symptom associated with osteoarthritis is prevented, ameliorated or improved.Type: ApplicationFiled: May 2, 2016Publication date: August 18, 2016Applicant: REGENERON PHARMACEUTICALS, INC.Inventors: Lynn MACDONALD, Richard TORRES, Marc R. MORRA, Joel H. MARTIN, Joel C. REINHARDT, Paul TISEO
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Patent number: 9353176Abstract: Methods are disclosed for treating osteoarthritis in a human subject in need thereof, comprising administering to the subject a therapeutically effective amount of an anti-human NGF antibody, or antigen-binding fragment thereof, wherein at least one symptom associated with osteoarthritis is prevented, ameliorated or improved.Type: GrantFiled: September 26, 2013Date of Patent: May 31, 2016Assignee: Regeneron Pharmaceuticals, Inc.Inventors: Lynn MacDonald, Richard Torres, Marc R. Morra, Joel H. Martin, Joel C. Reinhardt, Paul Tiseo
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Publication number: 20160003716Abstract: The present invention includes novel methods of processing a tissue sample. The present invention also includes novel methods of imaging a tissue sample. The present invention further includes a specimen holding device for performing the novel methods of processing a tissue sample and a microscope system for performing the novel methods of imaging a tissue sample.Type: ApplicationFiled: July 2, 2015Publication date: January 7, 2016Inventors: Richard Torres, Michael Levene
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Publication number: 20160003715Abstract: The present invention includes novel methods of processing a tissue sample. The present invention also includes novel methods of imaging a tissue sample.Type: ApplicationFiled: July 3, 2014Publication date: January 7, 2016Inventors: Richard Torres, Michael Levene