Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

Abstract: An attachment (10) for an inlet pipe (14) to a wet scrubber or other device comprises a sleeve (16) having an open end (18) adapted to be detachably connected to a flanged end (12) of the inlet pipe (14). A shaft (30) is moveable within the sleeve (16), and a scraper (34) is attached to one end of the shaft (30). The shaft (30) forms part of a pneumatic cylinder (40) attached to the other end of the sleeve (16). In use, the shaft (30) is reciprocally moved to cause the scraper (34) to dislodge particulates deposited within the pipe (14). Heated gas such as nitrogen or dry air is injected into the sleeve (16) to prevent scrubbing liquid from condensing within the sleeve (16) and thus inhibit particulate deposition therein.

Abstract: A snap lock modification which can be applied to various types of present day releasable ski bindings in which one component of the binding is connected to or forms a part of a ski boot and the other component is attached to the ski. One of the components includes latch means movable from inoperative position to latching position in engagement with a receiver formation on the other component. A resilient force unit is adjustable after the boot is in place to yieldingly urge the latch means into engaging position to retain the boot in position during normal maneuvers and to release the boot in response to excessive loads produced during a fall. The modification provides a detent trigger which restrains the action of the loaded force unit and which is actuatable upon placing the boot in skiing position to release and allow the latch means to snap into latching engagement with the receiver formation.

Abstract: This invention deals with composite articles of predetermined contour and with methods for their manufacture. The basic method involves providing a plurality of plies of a consolidated monotape, with each of the plies being precontoured in terms of size and degree of twist such that upon stacking the plies together, the plies provide a composite article of predetermined contour. The plies are stacked in predetermined relationship and then diffusion bonded together under heat and pressure to provide a complex shape such as a turbine engine blade to within close tolerances.

Abstract: A snap lock modification which can be applied to various types of present day releasable ski bindings in which one component of the binding is connected to or forms a part of a ski boot and the other component is attached to the ski. One of the components includes latch means movable from inoperative position to latching position in engagement with a receiver formation on the other component. A resilient force unit is adjustable after the boot is in place to yieldingly urge the latch means into engaging position to retain the boot in position during normal maneuvers and to release the boot in response to excessive loads produced during a fall. The modification provides a detent trigger which restrains the action of the loaded force unit and which is actuatable upon placing the boot in skiing position to release and allow the latch means to snap into latching engagement with the receiver formation.