Abstract: The present invention provides a method for producing biomolecular coatings on devices having gold surfaces. The method describes the production of recombinant fusion proteins consisting of one or more polypeptide domains of interest and a high affinity gold binding peptide consisting of 1 to 7 repeats of a gold binding protein (GBP) sequence. By this method, many biologically active polypeptides lacking intrinsic gold-binding properties can be firmly attached to gold surfaces. By exploiting such gold binding properties, devices are disclosed which comprise such coatings that are useful as prosthetic devices, implants, and tissue interfacing materials. Further, such devices comprising these coatings protect surfaces from fouling and impart various properties to the coated devices.

Abstract: The present invention provides a method to firmly attach any polypeptide to a gold surface regardless of its intrinsic gold-binding properties. The method describes the production of recombinant fusion proteins consisting of polypeptides of interest and a high affinity gold binding peptide consisting of 1 to 7 repeats of a unique amino acid sequence. By this method, many biologically active polypeptides lacking intrinsic gold-binding properties can be firmly attached to gold surfaces. The disclosure includes evidence that fusion proteins containing the gold-binding sequences provide superior stability and activity compared to similar molecules lacking the tag when used to construct biosensors. The invention provides a method that is a significant improvement over existing chemical and physical adsorption protocols to attach polypeptides to gold and, therefore, can provide benefits to many applications utilizing gold.

Abstract: The present invention provides libraries expressing peptide libraries on the extracellular cell surface of host cells and methods for identifying peptides that bind extracellular target molecules under the physiological conditions encountered in biological fluids and secretions. The present invention is also directed to vectors for expressing gene fusion proteins and for targeting those fusion proteins to the extracellular cell surface.

Abstract: The present invention provides a method to firmly attach any polypeptide to a gold surface regardless of its intrinsic gold-binding properties. The method describes the production of recombinant fusion proteins consisting of polypeptides of interest and a high affinity gold binding peptide consisting of 1 to 7 repeats of a unique amino acid sequence. By this method, many biologically active polypeptides lacking intrinsic gold-binding properties can be firmly attached to gold surfaces. The disclosure includes evidence that fusion proteins containing the gold-binding sequences provide superior stability and activity compared to similar molecules lacking the tag when used to construct biosensors. The invention provides a method that is a significant improvement over existing chemical and physical adsorption protocols to attach polypeptides to gold and, therefore, can provide benefits to many applications utilizing gold.

Abstract: A method of binding a recognition element to a gold surface (24) comprising the steps of attaching a GBP-AP protein (10) to a gold surface (24), digesting away the AP domain of the protein (10) with a proteolytic enzyme from the bound GBP domain (12) attached to the gold surface (24), and attaching a recognition element, such as Protein A (52) or an antibody 72, antigen, nucleic acid or other binding partner to the GBP domain (12) which remains on the gold surface (24), is disclosed.

Abstract: This application relates to modified blood coagulation factors, DNA sequences coding for such modified factors and a process for their production.

Abstract: A method is provided for expressing human alpha-1-antitrypsin in yeast utilizing a wild-type strain and a hyperproduction mutant, GK100.