Patents by Inventor Richie Soong
Richie Soong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8744777Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: GrantFiled: June 17, 2011Date of Patent: June 3, 2014Assignee: Roche Molecular Systems, Inc.Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Publication number: 20120270222Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: ApplicationFiled: June 17, 2011Publication date: October 25, 2012Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.Inventors: GREGOR SAGNER, KARIM TABITI, MARTIN GUTEKUNST, RICHIE SOONG
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Patent number: 8024132Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: GrantFiled: August 27, 2009Date of Patent: September 20, 2011Assignee: Roche Molecular SystemsInventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Publication number: 20100021923Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: ApplicationFiled: August 27, 2009Publication date: January 28, 2010Applicant: Roche Molecular Systems, Inc.Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Patent number: 7378241Abstract: The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.Type: GrantFiled: March 25, 2004Date of Patent: May 27, 2008Assignee: Roche Diagnostics Operations, Inc.Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Patent number: 7125691Abstract: The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.Type: GrantFiled: March 30, 2001Date of Patent: October 24, 2006Assignee: Roche Molecular Systems, Inc.Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Patent number: 7118867Abstract: The invention provides a method for quantitative multiplex PCR of two targets whereby one target is present in at least 100-fold molar excess over that of the other target. The method further comprises the addition of a thermostable DNA polymerase with a final concentration of at least 0.5 units/?l.Type: GrantFiled: November 19, 2002Date of Patent: October 10, 2006Assignees: Roche Diagnostics Corporation, Idaho Technology, Inc.Inventors: Karim Tabiti, Gisela Betzl, Richie Soong, Randy P. Rasmussen, Deepika Marine Desilva, John G. Ward, Hallegh Page Millward
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Publication number: 20050009048Abstract: The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.Type: ApplicationFiled: March 25, 2004Publication date: January 13, 2005Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Publication number: 20040153254Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: ApplicationFiled: December 24, 2003Publication date: August 5, 2004Applicant: Roche Molecular Systems, Inc.Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Patent number: 6691041Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: GrantFiled: March 30, 2001Date of Patent: February 10, 2004Assignee: Roche Molecular Systems, Inc.Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Publication number: 20030215830Abstract: The new invention provides a method and a kit for quantitative multiplex PCR with a high dynamic range characterized in that a thermostable DNA Polymerase with a final concentration of at least 0.5 units/&mgr;l is use.Type: ApplicationFiled: November 19, 2002Publication date: November 20, 2003Inventors: Karim Tabiti, Gisela Betzl, Richie Soong, Randy P. Rasmussen, Deepika Marine Desilva, John G. Ward, Haleigh Page Millward
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Publication number: 20030165832Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: ApplicationFiled: March 30, 2001Publication date: September 4, 2003Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Publication number: 20020058262Abstract: The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.Type: ApplicationFiled: March 30, 2001Publication date: May 16, 2002Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong