Abstract: Methods and kits for sequencing and quantification of small RNA molecules from small quantities of starting materials, including single cells, are disclosed. The invention can be applied to, among others, preparing small RNA libraries, synthesizing cDNA from small RNAs, characterizing small RNAs from various cell types, studying cellular heterogeneity in pathological conditions, and diagnosing diseases.

Abstract: A method for manufacturing a metallic component including the steps of providing a capsule, which defines at least a portion of the shape of the metallic component, arranging metallic material in the capsule, sealing the capsule, subjecting the capsule to Hot Isostatic Pressing for a predetermined time, at a predetermined pressure and at a predetermined temperature, and optionally, removing the capsule. The metallic material is at least one pre-manufactured coherent body, which pre-manufactured coherent body being made of metallic powder, wherein at least a portion of the metallic powder is consolidated such that the metallic powder is held together into a pre-manufactured coherent body. At least one portion of the pre-manufactured coherent body is manufactured by Additive Manufacturing by subsequently arranging superimposed layers of metallic powder.

Abstract: cDNA is prepared by hybridizing a cDNA synthesis primer to an RNA molecule and synthesizing a cDNA strand complementary to at least a portion of the RNA molecule to form an RNA-cDNA intermediate. A template switching reaction is performed by contacting the RNA-cDNA intermediate with a template switching oligonucleotide (TSO) under conditions suitable for extension of the cDNA strand using the TSO as template to form an extended cDNA strand complementary to the at least a portion of the RNA molecule and the TSO. The TSO comprises an amplification primer site, an identification tag, a UMI and multiple predefined nucleotides.

Abstract: Methods and kits for sequencing and quantification of small RNA molecules from small quantities of starting materials, including single cells, are disclosed. The invention can be applied to, among others, preparing small RNA libraries, synthesizing cDNA from small RNAs, characterizing small RNAs from various cell types, studying cellular heterogeneity in pathological conditions, and diagnosing diseases.

Abstract: A method for manufacturing a metallic component including the steps of providing a capsule, which defines at least a portion of the shape of the metallic component, arranging metallic material in the capsule, sealing the capsule, subjecting the capsule to Hot Isostatic Pressing for a predetermined time, at a predetermined pressure and at a predetermined temperature, and optionally, removing the capsule. The metallic material is at least one pre-manufactured coherent body, which pre-manufactured coherent body being made of metallic powder, wherein at least a portion of the metallic powder is consolidated such that the metallic powder is held together into a pre-manufactured coherent body. At least one portion of the pre-manufactured coherent body is manufactured by Additive Manufacturing by subsequently arranging superimposed layers of metallic powder.

Abstract: A method for manufacturing a metallic component including the steps of providing a capsule, which defines at least a portion of the shape of the metallic component, arranging metallic material in the capsule, sealing the capsule, subjecting the capsule to Hot Isostatic Pressing for a predetermined time, at a predetermined pressure and at a predetermined temperature, and optionally, removing the capsule. The metallic material is at least one pre-manufactured coherent body, which pre-manufactured coherent body being made of metallic powder, wherein at least a portion of the metallic powder is consolidated such that the metallic powder is held together into a pre-manufactured coherent body. At least one portion of the pre-manufactured coherent body is manufactured by Additive Manufacturing by subsequently arranging superimposed layers of metallic powder.

Abstract: This application discloses methods for cDNA synthesis with improved reverse transcription, template switching and preamplification to increase both yield and average length of cDNA libraries generated from individual cells. The new methods include exchanging a single nucleoside residue for a locked nucleic acid (LNA) at the TSO 3? end, using a methyl group donor, and/or a MgCl2 concentration higher than conventionally used. Single-cell transcriptome analyses incorporating these differences have full-length coverage, improved sensitivity and accuracy, have less bias and are more amendable to cost-effective automation. The invention also provides cDNA molecules comprising a locked nucleic acid at the 3?-end, compositions and cDNA libraries comprising these cDNA molecules, and methods for single-cell transcriptome profiling.

Abstract: A method for providing an inductive sealing bar, comprises providing a conductive coil having at least one heating zone, embedding the coil in a supportive body such that the supportive body covers the entire coil along at least some part of the length of the at least one heating zone; and providing a sealing surface of the sealing bar by planarizing the coil and the supportive body such that the coil is exposed along the entire length of said at least one heating zone. The invention also relates to a sealing bar manufactured according to the method.

Abstract: This application discloses methods for cDN?A synthesis with improved reverse transcription, template switching and preamplification to increase both yield and average length of cDNA libraries generated from individual cells. The new methods include exchanging a single nucleoside residue for a locked nucleic acid (INA) at the TSO 3? end, using a methyl group donor, and/or a MgCb concentration higher than conventionally used. Single-cell transcriptome analyses incorporating these differences have full-length coverage, improved sensitivity and accuracy, have less bias and are more amendable to cost-effective automation. The invention also provides cDNA molecules comprising a locked nucleic acid at the 3?-end, compositions and cDNA libraries comprising these cDNA molecules, and methods for single-cell transcriptome profiling.

Abstract: A method for manufacturing a metallic component including the steps of providing a capsule, which defines at least a portion of the shape of the metallic component, arranging metallic material in the capsule, sealing the capsule, subjecting the capsule to Hot Isostatic Pressing for a predetermined time, at a predetermined pressure and at a predetermined temperature, and optionally, removing the capsule. The metallic material is at least one pre-manufactured coherent body, which pre-manufactured coherent body being made of metallic powder, wherein at least a portion of the metallic powder is consolidated such that the metallic powder is held together into a pre-manufactured coherent body. At least one portion of the pre-manufactured coherent body is manufactured by Additive Manufacturing by subsequently arranging superimposed layers of metallic powder.

Abstract: A method for providing an inductive sealing bar, comprises providing a conductive coil having at least one heating zone, embedding the coil in a supportive body such that the supportive body covers the entire coil along at least some part of the length of the at least one heating zone; and providing a sealing surface of the sealing bar by planarizing the coil and the supportive body such that the coil is exposed along the entire length of said at least one heating zone. The invention also relates to a sealing bar manufactured according to the method.

Abstract: A method for manufacturing a component having at least one internal cavity includes the step of providing a core element of metallic material that includes at least one cavity having a first opening covered by a cover element with a first and second side. A form partially surrounds the core element and cover element. The form is filled with metallic filling material and heated in a heating chamber that is pressurized with gas for a predetermined time period at a predetermined temperature and a predetermined isostatic pressure, so that a metallurgical bond is achieved between the core element, cover element and metallic filling material. The core element is arranged such that, after filling the form with metallic filling material, the second side of the cover element is covered with metallic filling material so that the cavity during heating is pressurized to the predetermined isostatic pressure.

Abstract: This application discloses methods for cDN?A synthesis with improved reverse transcription, template switching and preamplification to increase both yield and average length of cDNA libraries generated from individual cells. The new methods include exchanging a single nucleoside residue for a locked nucleic acid (INA) at the TSO 3? end, using a methyl group donor, and/or a MgCb concentration higher than conventionally used. Single-cell transcriptome analyses incorporating these differences have full-length coverage, improved sensitivity and accuracy, have less bias and are more amendable to cost-effective automation. The invention also provides cDNA molecules comprising a locked nucleic acid at the 3?-end, compositions and cDNA libraries comprising these cDNA molecules, and methods for single-cell transcriptome profiling.

Abstract: A method for manufacturing a component having at least one internal cavity includes the step of providing a core element of metallic material that includes at least one cavity having a first opening covered by a cover element with a first and second side. A form partially surrounds the core element and cover element. The form is filled with metallic filling material and heated in a heating chamber that is pressurized with gas for a predetermined time period at a predetermined temperature and a predetermined isostatic pressure, so that a metallurgical bond is achieved between the core element, cover element and metallic filling material. The core element is arranged such that, after filling the form with metallic filling material, the second side of the cover element is covered with metallic filling material so that the cavity during heating is pressurized to the predetermined isostatic pressure.

Abstract: The present invention relates to a method of making tungsten carbide powder by dissolving at least one organic or inorganic metal salt or compound of at least one of the groups IV, V, and VI of the periodic system preferably Cr, V, Mo and W in at least one polar solvent. Powder of WO3 is added to the solution, the solvent is evaporated, the remaining powder is heat treated in reducing atmosphere, mixed with carbon and carburized.

Abstract: A method of making cemented carbide at which powders forming hard constituents and powders forming binder phase are wet milled together with a pressing agent is disclosed. The slurry is dried, preferably by spray drying, compacted into bodies of desired shape and sintered. A cemented carbide powder with a reduced compacting pressure at a predetermined weighing in of 18% shrinkage can be obtained by using from about 1 to about 3 wt-% pressing agent with the following composition: less than about 90 wt-% PEG and greater than about 10 wt-% of long chain C?20 fatty acids, their esters and salts, in particular, erucic acid and/or behenic acid. The invention also relates to a cemented carbide powder with low compaction pressure.

Abstract: A method of making cemented carbide at which powders forming hard constituents and powders forming binder phase are wet milled together with a pressing agent is disclosed. The slurry is dried, preferably by spray drying, compacted into bodies of desired shape and sintered. A cemented carbide powder with a reduced compacting pressure at a predetermined weighing in of 18% shrinkage can be obtained by using from about 1 to about 3 wt-% pressing agent with the following composition: less than about 90 wt-% PEG and greater than about 10 wt-% of long chain C?20 fatty acids, their esters and salts, in particular, erucic acid and/or behenic acid. The invention also relates to a cemented carbide powder with low compaction pressure.

Abstract: The present invention relates to a method of making tungsten carbide powder by dissolving at least one organic or inorganic metal salt or compound of at least one of the groups IV, V, and VI of the periodic system preferably Cr, V, Mo and W in at least one polar solvent. Powder of WO3 is added to the solution, the solvent is evaporated, the remaining powder is heat treated in reducing atmosphere, mixed with carbon and carburized.