Abstract: A method of investigating a DNA sample is provided involving taking at least two sub-samples from the sample, amplifying the sub-samples, analyzing the sub-samples to obtain identity information and amount of that identity about one or more alleles indicated as present in the sub-samples in respect of 5 or more loci and establishing identity information deemed representative of the sample from the sub-samples, wherein identity information from a sub-sample about a particular identity is included in the identity information deemed representative of the sample when that particular identity is indicated as present by one or more of the sub-samples analyzed, the amount of that particular identity in the identity information deemed representative of the sample being a weighted combination based on the amount of that particular identity in the sub-samples. In this way useful profiles can be obtained more often than they are at present, with reduced costs and with reduced expertise and time requirements.