Abstract: The present invention relates to an isolated polypeptide having xylanase activity, selected from the group consisting of: a) a polypeptide comprising an amino acid sequence having at least 87% identity with SEQ ID NO: 1; b) a polypeptide encoded by a polynucleotide having at least 87% identity with SEQ ID NO:2; or c) a fragment of a polypeptide of a) or b) which fragment has xylanase activity.

Abstract: The present invention provides hemicellulytic enzyme variants. Specifically, the present invention provides xylanase variants having one or more modifications as compared to a parent xylanase enzyme resulting in at least one improved property. In addition, the present invention provides compositions comprising a xylanase variant of the invention. The present invention also provides methods of degrading hemicellulotic material, including arabino-xylan using compositions comprising a xylanase variant of the invention.

Abstract: A GH10 xylanase variant is disclosed herein. This GH10 xylanase variant has increased thermostability compared with a parent GH10 xylanase enzyme comprising at least 90% sequence identity with the amino acid sequence of SEQ ID No. 1 wherein the variant has been modified at two or more of the following positions 7, 33, 79, 217 and 298 corresponding to the amino acid numbering of SEQ ID No. 1.

Abstract: The present invention relates to an isolated polypeptide having xylanase activity, selected from the group consisting of: a) a polypeptide comprising an amino acid sequence having at least 87% identity with SEQ ID NO: 1; b) a polypeptide encoded by a polynucleotide having at least 87% identity with SEQ ID NO:2; or c) a fragment of a polypeptide of a) or b) which fragment has xylanase activity.

Abstract: The present inventions relates to a modified GH10 xylanase enzyme or a fragment thereof having xylanase activity wherein said modified GH10 xylanase or fragment thereof has increased thermostability compared with a parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at, at least, two of the following positions 7, 33, 79, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). In particular, the modified xylanase enzyme according to the present invention has the following modifications: N7D; T33V; K79Y, V, F, I, L or M (preferably K79Y, F or V, more preferably Y); A217Q, E, P, D or M (preferably A217Q, E or P, more preferably Q); and T298Y, F or W (preferably Y or F, more preferably Y).

Abstract: A process for the preparation of a corn-based foodstuff, the process comprising the step of contacting a corn-based flour with a xylanase enzyme, as defined herein, such that a xylan-containing material native to the corn is degraded, is disclosed. Flours, masas and masa foodstuffs, especially tortillas, produced by the process are also disclosed.

Abstract: The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of the heterologous nucleotide sequence(s) encoding the lipolytic enzyme.

Abstract: The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of said heterologous nucleotide sequence(s) encoding said lipolytic enzyme.

Abstract: The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO:2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of the heterologous nucleotide sequence(s) encoding the lipolytic enzyme.

Abstract: The present invention relates to a feed supplement comprising a phytase and a lipolytic enzyme, wherein said lipolytic enzyme has lipase activity at a pH in the range of about pH1.5 to about pH3.5.

Abstract: The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO:2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of said heterologous nucleotide sequence(s) encoding said lipolytic enzyme.

Abstract: The present invention relates to a feed supplement comprising a phytase and a lipolytic enzyme, wherein said lipolytic enzyme has lipase activity at a pH in the range of about pH1.5 to about pH3.5.

Abstract: The present invention relates to a method for the production of a lipid acyltransferase comprising the steps of: (i) providing a Bacillus licheniformis cell; (ii) transforming the Bacillus licheniformis cell with a heterologous nucleotide sequence encoding a lipid acyltransferase; and (iii) expressing the lipid acyltransferase in the cell under the control of a promoter sequence. In addition, the present invention further relates to the use of Bacillus licheniformis to express a lipid acyltransferase, a Bacillus licheniformis host cell comprising a heterologous lipid acyltransferase and a vector comprising a nucleotide sequence encoding a lipid acyltransferase operably linked to a promoter sequence homologous to B. licheniformis.

Abstract: The present invention relates to a method for the production of a lipid acyltransferase comprising the steps of: (i) providing a Bacillus licheniformis cell; (ii) transforming the Bacillus licheniformis cell with a heterologous nucleotide sequence encoding a lipid acyltransferase; and (iii) expressing the lipid acyltransferase in the cell under the control of a promoter sequence. In addition, the present invention further relates to the use of Bacillus licheniformis to express a lipid acyltransferase, a Bacillus licheniformis host cell comprising a heterologous lipid acyltransferase and a vector comprising a nucleotide sequence encoding a lipid acyltransferase operably linked to a promoter sequence homologous to B. licheniformis.