Abstract: The invention relates to a method of treating chronic granulomatous disease through Hematopoietic stem cell (HSC) transplantation. The method comprises the steps of administering stem cell mobilization agent to human such that the target stem cell population migrates from host niches into the subject's blood. The target stem cells are removed from blood and administering the therapeutic stem cells to human and said therapeutic stem cells are engineered to express gp91phox. The steps are repeated multiple times i.e. at least four times. The mobilization agents used in the invention are granulocyte-colony stimulating factor and AMD3100. The method of HSC transplantation is effective in treatment of chronic granulomatous disease.

Abstract: Printing devices include a printing engine and a media tray connected to a frame. The media tray is adapted to hold a stack of media supplied to the printing engine for printing. A magnetic trailing edge guide that is shaped to contact a corner of the stack of media is connected to a tether that is connected to the frame. A storage device is connected to the frame, and the storage device is shaped to accommodate the magnetic trailing edge guide. The length of the tether allows the magnetic trailing edge guide to reach the corner of the stack of media and to reach the storage device. The magnetic trailing edge guide includes a magnetic element adapted to magnetically hold to at least the media tray and the storage device.

Abstract: A method of operating an additive manufacturing system feeds solid extrusion material into a heater using a slip clutch coupled to an actuator of a mechanical driver to supply thermoplastic material into a manifold in an extruder head. The method sets a speed of the actuator so the actuator operates at a rotational speed that is slightly greater than the rotational speed of the mechanical mover. This method helps maintain the pressure of the thermoplastic material in the manifold of the extruder head in a predetermined range no matter how many nozzles are opened in the extruder head.

Abstract: A cap is positioned to contact a printhead when the printhead is not ejecting liquid ink. The cap and the printhead create a sealed space adjacent printhead nozzles when contacting each other. A flexible blade is positioned to contact the printhead when the printhead is not in contact with the cap. The flexible blade is adapted to fold over to spread a liquid solution on the nozzles in a first direction, and the flexible blade is adapted to remove excess amounts of the liquid solution from the nozzles in a second direction. A humidifier is connected to the cap and adapted to supply a moisture form of the liquid solution to the sealed space. A moisture sensor is connected to the cap. The humidifier is adapted to vary supply of the moisture to the sealed space based on the amount of moisture detected by the moisture sensor.

Abstract: A three-dimensional (3-D) printer includes build and support material development stations positioned to transfer layers of build and support materials to an intermediate transfer surface. The intermediate transfer surface transfers a layer of the build and support materials to a platen each time the platen contacts the intermediate transfer surface. A sensor detects the thickness of the layer on the platen, and a mechanical planer is positioned to contact and level the layer on the platen as the platen moves past the mechanical planer. Additionally, a feedback loop is electrically connected to the sensor and the mechanical planer. The mechanical planer adjusts the amount of the build material and the support material removed from the layer based on the thickness of the layer on the platen, as determined by the sensor.

Abstract: Printing devices include a printing engine and a media tray connected to a frame. The media tray is adapted to hold a stack of media supplied to the printing engine for printing. A magnetic trailing edge guide that is shaped to contact a corner of the stack of media is connected to a tether that is connected to the frame. A storage device is connected to the frame, and the storage device is shaped to accommodate the magnetic trailing edge guide. The length of the tether allows the magnetic trailing edge guide to reach the corner of the stack of media and to reach the storage device. The magnetic trailing edge guide includes a magnetic element adapted to magnetically hold to at least the media tray and the storage device.

Abstract: Devices include one or more inkjet printheads, a reusable jetting sheet that is adjacent to nozzles of the printheads, support structures (e.g., drive rollers) contacting the sheet, and a cleaning station contacting the sheet. The reusable jetting sheet includes an opening, that is at least as large as the sets of nozzles, and a jetting area spaced from the opening. The support structures are adapted to move the reusable jetting sheet relative to the sets of nozzles. The inkjet printheads are adapted to eject ink from at least some of the nozzles, through the opening, to print media when the opening is positioned adjacent to the sets of nozzles, to print on print media. The inkjet printhead is further adapted to eject ink from at least some of the nozzles to the jetting area when the jetting area is positioned adjacent to the sets of nozzles to perform maintenance jetting.

Abstract: Certain embodiments are directed to compositions and methods for non-cytotoxic hematopoietic stem cell transplantation.

Abstract: Devices include one or more inkjet printheads, a reusable jetting sheet that is adjacent to nozzles of the printheads, support structures (e.g., drive rollers) contacting the sheet, and a cleaning station contacting the sheet. The reusable jetting sheet includes an opening, that is at least as large as the sets of nozzles, and a jetting area spaced from the opening. The support structures are adapted to move the reusable jetting sheet relative to the sets of nozzles. The inkjet printheads are adapted to eject ink from at least some of the nozzles, through the opening, to print media when the opening is positioned adjacent to the sets of nozzles, to print on print media. The inkjet printhead is further adapted to eject ink from at least some of the nozzles to the jetting area when the jetting area is positioned adjacent to the sets of nozzles to perform maintenance jetting.

Abstract: A cap is positioned to contact a printhead when the printhead is not ejecting liquid ink. The cap and the printhead create a sealed space adjacent printhead nozzles when contacting each other. A flexible blade is positioned to contact the printhead when the printhead is not in contact with the cap. The flexible blade is adapted to fold over to spread a liquid solution on the nozzles in a first direction, and the flexible blade is adapted to remove excess amounts of the liquid solution from the nozzles in a second direction. A humidifier is connected to the cap and adapted to supply a moisture form of the liquid solution to the sealed space. A moisture sensor is connected to the cap. The humidifier is adapted to vary supply of the moisture to the sealed space based on the amount of moisture detected by the moisture sensor.

Abstract: Certain embodiments are directed to compositions and methods for non-cytotoxic hematopoietic stem cell transplantation.

Abstract: A method of operating an additive manufacturing system feeds solid extrusion material into a heater using a slip clutch coupled to an actuator of a mechanical driver to supply thermoplastic material into a manifold in an extruder head. The method sets a speed of the actuator so the actuator operates at a rotational speed that is slightly greater than the rotational speed of the mechanical mover. This method helps maintain the pressure of the thermoplastic material in the manifold of the extruder head in a predetermined range no matter how many nozzles are opened in the extruder head.

Abstract: A 3-D printer includes build and support material development stations that electrostatically transfer build material and support material to an ITB. The ITB transfers a layer of build and support material to a platen each time the platen contacts one of the layers on the ITB, to successively form a freestanding stack of the layers on the platen. A sensor is positioned to generate a topographic measurement of the layer on the platen, and an aerosol applicator is positioned to propel build and support material on to the layer on the platen. The aerosol applicator controls the build and support material being propelled, based on the topographic measurement from the sensor through a feedback loop, to adjust the amount and location of the build material and the support material propelled on to the layer, and thereby control the flatness of surface topology of the layers in the freestanding stack on the platen.

Abstract: An additive manufacturing system includes a slip clutch coupled to an actuator of a mechanical driver that feeds solid extrusion material into a heater for supplying thermoplastic material to a manifold in an extruder head. A speed of the actuator can be set to enable the actuator to operate at a rotational speed that is slightly greater than the rotational speed of the mechanical mover. This configuration enables the pressure of the thermoplastic material in the manifold of the extruder head to be in a predetermined range no matter how many nozzles are opened in the extruder head.

Abstract: A method of additive manufacturing includes forming a plurality of build layers, each of the plurality of build layers formed by transferring a first build material having a first particle size to form a first build material and transferring a second build material on the first build material to form one of the plurality of build layers, a particle size of the second build material is smaller than the first build material and each transfer step is performed by a xerographic engine. Each transfer step is involves transfer to a conveyor which can take the form of a belt or drum.

Abstract: A three-dimensional (3-D) printer includes build and support material development stations positioned to transfer layers of build and support materials to an intermediate transfer surface. The intermediate transfer surface transfers a layer of the build and support materials to a platen each time the platen contacts the intermediate transfer surface. A sensor detects the thickness of the layer on the platen, and a mechanical planer is positioned to contact and level the layer on the platen as the platen moves past the mechanical planer. Additionally, a feedback loop is electrically connected to the sensor and the mechanical planer. The mechanical planer adjusts the amount of the build material and the support material removed from the layer based on the thickness of the layer on the platen, as determined by the sensor.

Abstract: Certain embodiments are directed to compositions and methods for non-cytotoxic hematopoietic stem cell transplantation for the treatment of chronic granulomatous disease

Abstract: A three-dimensional (3-D) printer includes build and support material development stations positioned to transfer layers of build and support materials to an intermediate transfer surface. The intermediate transfer surface transfers a layer of the build and support materials to a platen each time the platen contacts the intermediate transfer surface. A sensor detects the thickness of the layer on the platen, and a mechanical planer is positioned to contact and level the layer on the platen as the platen moves past the mechanical planer. Additionally, a feedback loop is electrically connected to the sensor and the mechanical planer. The mechanical planer adjusts the amount of the build material and the support material removed from the layer based on the thickness of the layer on the platen, as determined by the sensor.

Abstract: An apparatus is disclosed that includes a computer operated cutting and creasing tool configured to move only in an X direction during use, a cutting and creasing platform having an elastically deformable creasing portion configured to support a sheet of media during contact with a creasing tip and a non-deformable cutting portion configured to support the sheet during contact with a cutting blade, and a positioner configured to draw the sheet of media along the cutting and creasing platform during cutting and creasing. Methods of making and using the apparatus also are disclosed.

Abstract: 3-D printers include an intermediate transfer surface that transfers a layer of material to a platen each time the platen contacts the intermediate transfer surface to successively form a freestanding stack of layers of the material on the platen. A sensor detects the thickness of the layer on the platen after a fusing station fuses the layer. A feedback loop is electrically connected to the sensor and a development station (that includes a photoreceptor, a charging station providing a static charge to the photoreceptor, a laser device exposing the photoreceptor, and a development device supplying the material to the photoreceptor). The development station adjusts the development bias of the development device, based on a layer thickness measurement from the sensor through the feedback loop, to control the thickness of subsequent ones of the layers transferred from the intermediate transfer surface to the freestanding stack on the platen.