Abstract: A method for detecting a target cell surface molecule and classifying cell types in a fluid sample. The method involves the addition of a reagent to the fluid sample. The reagent includes nanoparticles with optical plasmonic resonances, and at least one fluorescent probe. The nanoparticles are a bio-optical probe for the target cell surface molecule. Each fluorescent probe targets a cell classification marker. The method further involves the acquisition of an image using dark field microscopy and fluorescence microscopy to detect and quantify the presence or absence of any cells in the fluid sample having the target cell surface molecule or having the cell classification marker.

Abstract: A method for detecting a target cell surface molecule and classifying cell types in a fluid sample. The method involves the addition of a reagent to the fluid sample. The reagent includes nanoparticles with optical plasmonic resonances, and at least one fluorescent probe. The nanoparticles are a bio-optical probe for the target cell surface molecule. Each fluorescent probe targets a cell classification marker. The method further involves the acquisition of an image using dark field microscopy and fluorescence microscopy to detect and quantify the presence or absence of any cells in the fluid sample having the target cell surface molecule or having the cell classification marker.

Abstract: The present invention reagents and methods for setting up an instruments having a multiplicity of detector channels for analyzing a multiplicity of fluorescent dyes. The present invention is particularly applicable in the field of flow cytometry.

Abstract: The present invention reagents and methods for setting up an instruments having a multiplicity of detector channels for analyzing a multiplicity of fluorescent dyes. The present invention is particularly applicable in the field of flow cytometry.

Abstract: The present invention reagents and methods for setting up an instruments having a multiplicity of detector channels for analyzing a multiplicity of fluorescent dyes. The present invention is particularly applicable in the field of flow cytometry.

Abstract: The present invention reagents and methods for setting up an instruments having a multiplicity of detector channels for analyzing a multiplicity of fluorescent dyes. The present invention is particularly applicable in the field of flow cytometry.

Abstract: A trail marking system having a plurality of trail markers, a remote control handset for selectively activating the trail markers, and a setting tool for setting and removing the trail markers. To use the trail marking system, a hunter attaches a trail marker into a tree using the setting tool. The trail markers contain externally perceivable indicators that can be selectively turned on an off. When the indicators in the trail markers are off, the trail markers are inconspicuous. However, when the indicators are activated, they are highly visible. The trail markers are selectively turned on an off using the remote control handset. As such, a hunter can selectively turn on and off the trail markers as he/she walks along the trail.

Abstract: An apparatus and method for examining particles in a flow stream of a flow cytometer, employing incoherent light sources, such as light emitting diodes (LEDs), and detectors. The light emitting diodes operate as the excitation light sources and emit light toward said flow stream, and the detectors detect light, in particular, fluorescent light, emanating from the particles in response to the excitation light striking the particles. A controller controls each of the light emitting diodes to emit their excitation light for a predetermined period during which the excitation light radiates onto particles of interest. The controller evaluates the detected light to ascertain characteristics of the particles, such as particle size, density and granularity. The apparatus and method can further employ one or more coherent and homogenous light emitting devices, such as a laser, as an additional excitation light source.

Abstract: This invention presents improved methodology for identification of nucleated cells in flow cytometric analysis when immunofluorescent dyes are also used. Briefly, in the method nucleic acids are stained with a fluorescent dye which can then be used to identify the nucleated cells by measurement of fluorescence on a flow cytometer. The improvement presented by this invention is the use of a saturating (or near saturating) amount of a nucleic acid dye, or mixture of dyes, which gives low fluorescence at excitation conditions, so as not to greatly interfere with the signals of the immunofluorescent dyes.

Abstract: An apparatus for thermal extrusion of a polymeric material to manufacture prototype mechanical elements from said polymeric material, said apparatus used for deposition of a liquefied ribbon of said polymeric material in patterned layers which solidify to form a three-dimensional prototype element, said apparatus comprising in combination, a mounting frame, said mounting frame including a mounting plate with a movable platen movable in the x and y directions, said frame further including an extrusion cylinder, said extrusion cylinder comprising a cylindrical housing having a uniform diameter cylindrical through passage, a head member having a uniform diameter bore connected with a reduced diameter, integral tip opening, said head member attached by a clamp to one end of the cylindrical housing to align the bore with the through passage, said-bore and through passage having an equal diameter, said head member including a circumferential heater to liquefy contents of the head member, a reciprocal piston slidabl

Abstract: A multi-laser flow-cytometry system provides for inter-location emissions crosstalk cancellation, through either pulse subtraction or gating, to avoid false triggering based on shadow (pre- and post-) pulses that occur when red-excited red emissions are detected by a photodetector arranged to detect blue-excited red emissions. This overcomes a problem of undercounting of counting beads that led to misdeterminations of cell counts when blue-excited red fluorescence detections were used as a trigger. The approach also provides for more accurate quantitative data regarding fluorochrome emissions amplitudes.

Abstract: A fluorescence particle analyzer includes a flow tube through which particles marked with different fluorochromes pass from a first location to a second location. Emissions from the first and second locations are respectively imaged at respective first and second filter elements so that emissions that reach a photodetector from said first location are primarily filtered by the first filter element, while emission that reach the same photodetector from the second location are primarily filtered by the second filter element. The first filter element is selected to preferentially transmit emissions from a first fluorochrome at the expense of emissions from a second fluorochrome, while the second filter element is selected to preferentially transmit emissions from the second fluorochrome at the expense of emissions from the first fluorochrome.

Abstract: A method and apparatus for identifying individual particles or cells which have been labeled with different fluorochromes, on the basis of the lifetime of their fluorescence, or based on different decay times for a fluorochrome in different cells.

Abstract: A flow cytometer measures phase fluorescence lifetimes by the phase shift of a reference signal and an emission from a particle or cell in a flow chamber. An acoustic optic modulator modulates laser light with a sinusoidal wave of a predetermined frequency to excite particles or cells. Detectors respond to emissions of individual particles or cells in the form of an output signal pulse at the predetermined frequency. The output signal pulse is divided into equal pulses with each at the modulation frequency, the same amplitude and fidelity and amplitude. One part of the divided pulse is stripped of its envelope to pass the width thereof and out of band components are rejected. A variable amplifier passes a portion of the pulse above a present level. A delay line sets a central part of the signal at a predetermined point in time. A circuit limits the attenuated one part. A double balance mixer multiplies and the relates the limited signal with a reference signal to determine the phase shift.

Abstract: A method for cleaning cellular contamination in a flow cytometer comprising the sequential use of a strong oxidizing solution, particle-free neutral pH fluid and a weak acid.

Abstract: A method and apparatus for cleaning cellular contamination in flow cytometer comprising the sequential use of a strong oxidizing solution, particle-free neutral pH fluid and a weak acid.

Abstract: A tractor has a typical three-point hitch with lower lift arms and an upper stabilizing arm comprising, in part, of a transverse horizontal rod extending between the lift arms. A pair of connecting structures are mounted on the implement and spaced on the order of the lift arms but being transversely offset therefrom. Each of the connecting structures includes a vertical plate having a U-shaped edge opening vertically for receiving the transverse bar. A link is mounted on the fore-and-aft plate and extends from one position in which it traverses the opening of the U so as to retain the bar in the nip area of the U-shaped edge and a position in which it extends vertically and opens up the U-shaped edge so that the bar may have ingress and egress from the nip area. A latching rod extends from the plate and there is provided an opening in the link that receives the latching rod.

Abstract: Samples are prepared and applied to a cuvette, and the cuvette is translated in a given direction. Laser light is coupled to the sample through a Bragg cell, which causes the beam to scan the sample transversely to the given direction. Select optical parameters are monitored, and when they occur, the scan is stopped or slowed for a more complete analysis of the illuminated cell.

Abstract: Red cell samples are passed through a zone of focused illumination, and a scatter vs. time pulse is obtained for each. Each pulse is analyzed for pulse width, pulse area, and pulse shape. These parameters are respectively collected, and three coefficients of variation are obtained for each such collection. In addition to these three abnormality factors, the mean of the pulse shape collection constitutes a fourth. The four are respectively subject to statistical criteria, and are combined in predetermined fashion for a single red cell morphology index.

Abstract: Methods for the determination of phagocytic and killing ability of selected leukocyte subclasses based upon the use of three part differentials obtained by light scatter characteristics and gated fluorescence. Particles having multiple fluorescent labels associated therewith, one of which is sensitive to reactive oxygen, incubated with the leukocytes. Detection of the fluorescent labels permits identification of cells having phagocytic ability, as determined by presence of the non-sensitive label, and also the identification of those with killing ability as determined by the decrease in fluorescence from the sensitive label.