Abstract: Among other things, this disclosure provides a method of detecting a target nucleic acid. Aspects of the method include: (a) obtaining a labeled nucleic acid probe that is complementary to a target nucleic acid, wherein the probe comprises a capture tag; (b) hybridizing the probe with the target nucleic in a fixed cell, in situ, to produce a duplex; (c) linking the probe in the duplex to a peroxidase conjugate via the capture tag to produce a peroxidase-labeled duplex; and (d) incubating the peroxidase-labeled duplex with a peroxidase substrate, wherein the peroxidase activity of the peroxidase conjugate catalyzes deposition of the substrate in the vicinity of the duplex, thereby producing a detectable signal.

Abstract: Among other things, this disclosure provides a method of detecting a target nucleic acid. Aspects of the method include: (a) obtaining a labeled nucleic acid probe that is complementary to a target nucleic acid, wherein the probe comprises a capture tag; (b) hybridizing the probe with the target nucleic in a fixed cell, in situ, to produce a duplex; (c) linking the probe in the duplex to a peroxidase conjugate via the capture tag to produce a peroxidase-labeled duplex; and (d) incubating the peroxidase-labeled duplex with a peroxidase substrate, wherein the peroxidase activity of the peroxidase conjugate catalyzes deposition of the substrate in the vicinity of the duplex, thereby producing a detectable signal.

Abstract: Nucleic acid arrays that include a set of hybridization parameter probe features are provided. Also provided are methods of using the subject arrays in hybridization assays. In certain aspects, signals detected from the hybridization parameter probe features may be employed to determine a hybridization parameter of the assay. The subject arrays and methods find use in a variety of different applications. Also provided are computer programming, devices that include the same and kits that find use in practicing the subject methods.

Abstract: The present invention relates to methods for detecting, quantitating, or standardizing binding to a nucleic acid array, standard polynucleotides that can be used in such methods, compositions including the standard polynucleotides, and methods of making the standard polynucleotides. An aspect of the present invention relates to a complete exon transcript of a gene. The gene can be a gene expressed as multiple transcripts but encoding only transcripts lacking at least a portion of at least one exon. A complete exon transcript includes all of the known bases of every known exon for such a gene. An aspect of the present invention relates to a method of detecting binding to a nucleic acid array. The method can include contacting the nucleic acid array with a standard polynucleotide for a gene. The standard polynucleotide for a gene includes all of the known bases of every known exon for that gene. In an embodiment, the standard polynucleotide includes a complete exon transcript of the gene.

Abstract: The invention provides methods and sensors for detecting target biological molecules. Biosensors feature photoactivatable charge separation moieties capable of generating electron-hole pairs upon photoinduction. Photoinduced charge carriers participate in redox reactions that are detectable, for example, by optical, chemical, or electronic means.