Patents by Inventor Robert C. Boguslaski
Robert C. Boguslaski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 5420016Abstract: A rapid method and easy to use unitized test device is disclosed for determining the presence of Helicobacter pylori in a biological tissue specimen by detecting the presence of urease in the tissue. The system basically utilizes a multilayer test device for separating and optimizing the various reactions involved, i.e. the urease in the specimen with a substrate and the ammonia generated thereby with an indicator element.Type: GrantFiled: February 18, 1994Date of Patent: May 30, 1995Assignee: Serim Research CorporationInventors: Robert C. Boguslaski, Robert J. Carrico
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Patent number: 5314804Abstract: A rapid method and easy to use unitized test device is disclosed for determining the presence of Helicobacter pylori in a biological tissue specimen by detecting the presence of urease in the tissue. The system basically utilizes a multilayer test device for separating and optimizing the various reactions involved, i.e. the urease in the specimen with a substrate and the ammonia generated thereby with an indicator element.Type: GrantFiled: March 24, 1992Date of Patent: May 24, 1994Assignee: Serim Research CorporationInventors: Robert C. Boguslaski, Robert J. Carrico
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Patent number: 4791055Abstract: The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction is affected by reaction between the specific binding substance in the conjugate and a specific binding counterpart thereto. The presence of a ligand in a liquid medium may be determined using competitive or displacement binding or sequential saturation techniques wherein the specific binding substance in the conjugate is the ligand or a specific binding analog thereof, or using a direct binding technique wherein the specific binding substance is a specific binding partner of the ligand. The effect of the specific binding reaction on the activity of the conjugated reactant is related to the presence or amount of the ligand in the liquid medium tested.Type: GrantFiled: January 9, 1986Date of Patent: December 13, 1988Assignee: Miles Inc.Inventors: Robert C. Boguslaski, Robert J. Carrico, James E. Christner
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Patent number: 4492751Abstract: An improved heterogenous specific binding assay method which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium. The method is carried out using reagent means which comprises, as its labeled constituent, a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction system is utilized as means for monitoring the extent of binding of the labeled constituent in conventional heterogenous specific binding assay schemes. The presence of a ligand in a liquid medium may be determined following conventional competitive binding manipulative techniques.Type: GrantFiled: October 15, 1979Date of Patent: January 8, 1985Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, Robert J. Carrico, James E. Christner
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Patent number: 4404366Abstract: An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula:G--D--Rwherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glycosidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or heterogeneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue.Type: GrantFiled: July 16, 1981Date of Patent: September 13, 1983Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, John F. Burd, Robert J. Carrico
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Patent number: 4383031Abstract: A homogeneous specific binding assay which employs a substance having reactant activity, i.e., a reactant, in a chemiluminescent reaction as a labeling substance in the detection of a ligand in a liquid medium. The assay employs a conjugate formed of a specific binding substance coupled to the chemiluminescent reactant. The activity of the conjugated reactant as a constituent of the chemiluminescent reaction is affected by reaction between the specific binding substance in the conjugate and a specific binding counterpart thereto. The presence of a ligand in a liquid medium may be determined using competitive or displacement binding or sequential saturation techniques wherein the specific binding substance in the conjugate is the ligand or a specific binding analog thereof, or using a direct binding technique wherein the specific binding substance is a specific binding partner of the ligand.Type: GrantFiled: June 21, 1979Date of Patent: May 10, 1983Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, Robert J. Carrico
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Patent number: 4380580Abstract: A heterogeneous specific binding assay which employs a substance having reactant activity, i.e., a reactant, in a chemiluminescent reaction as a labeling substance in the detection of a ligand in a liquid medium. The method is carried out using reagent means which comprises, as its labeled constituent, a conjugate formed of a specific binding substance coupled to the chemiluminescent reactant. The activity of the conjugated reactant in the chemiluminescent reaction, i.e., the production of light, is utilized as means for monitoring the extent of binding of the labeled constituent in conventional heterogeneous specific binding assay schemes. The presence of a ligand in a liquid medium may be determined following conventional competitive binding manipulative techniques.Type: GrantFiled: June 21, 1979Date of Patent: April 19, 1983Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, Robert J. Carrico, James E. Christner
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Patent number: 4363759Abstract: Chemiluminescent-labeled conjugates of the formula: ##STR1## wherein one of R.sup.1 and R.sup.2 is hydrogen and the other is -NR.sup.3 R.sup.4 ; R.sup.3 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and R.sup.4 is ##STR2## wherein n=1-3 and L(CO-- is the ligand or analog bound through an amide bond. Intermediates produced in the synthesis of such conjugates are also disclosed.Type: GrantFiled: July 24, 1978Date of Patent: December 14, 1982Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, Robert J. Carrico, James E. Christner
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Patent number: 4355165Abstract: Amino-functionalized phthalhydrazide intermediates of the formula: ##STR1## wherein one of R.sup.5 and R.sup.6 is hydrogen and the other is --NR.sup.7 R.sup.8 ; R.sup.7 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and R.sup.8 is ##STR2## wherein n=1-3. The compounds are intermediates in the synthesis of chemiluminescent-labeled conjugates which are useful as reagents in specific binding assays for determining ligands or their specific binding partners in liquid media.Type: GrantFiled: March 20, 1980Date of Patent: October 19, 1982Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, Robert J. Carrico, James E. Christner
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Patent number: 4318980Abstract: An improved heterogenous specific binding assay method which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium. The method is carried out using reagent means which comprises, as its labeled constituent, a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction system is utilized as means for monitoring the extent of binding of the labeled constituent in conventional heterogenous specific binding assay schemes. The presence of a ligand in a liquid medium may be determined following conventional competitive binding manipulative techniques.Type: GrantFiled: October 15, 1979Date of Patent: March 9, 1982Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, Robert J. Carrico, James E. Christner
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Patent number: 4279992Abstract: An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula:G--D--Rwherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glycosidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or heterogeneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue.Type: GrantFiled: October 23, 1979Date of Patent: July 21, 1981Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, John F. Burd, Robert J. Carrico
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Patent number: 4261893Abstract: Bis-phthalimide intermediates of the formula: ##STR1## wherein one of R.sup.9 and R.sup.10 is hydrogen and the other is --NR.sup.11 R.sup.12 ; R.sup.11 is hydrogen or straight stain alkyl containing 1-4 carbon atoms and R.sup.12 is ##STR2## wherein n=1-3. The compounds are intermediates in the synthesis of chemiluminescent-labeled conjugates which are useful as reagents in specific binding assays for determining ligands or their specific binding partners in liquid media.Type: GrantFiled: August 20, 1979Date of Patent: April 14, 1981Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, Robert J. Carrico
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Patent number: 4238195Abstract: A specific binding assay method and reagent means for determining a ligand, such as an antigen or antibody, in, or the ligand binding capacity of, a liquid medium which employs a fluorescent substance, i.e., a fluorescer, as a label. The improvement comprises measuring the fluorescer-label by chemically exciting the label and measuring the resulting light emitted thereby. Chemical excitation of the label is preferably accomplished by exposure to a substance, such as a high energy intermediate produced by the reaction between hydrogen peroxide and highly reactive materials such as oxalyl chloride, oxamides and bis-oxalate esters. The assay may follow conventional homogeneous and heterogeneous formats. The improved assay is more convenient than conventional fluorescent binding assays by obviating the need for photogenic excitation of the fluorescent label.Type: GrantFiled: January 18, 1979Date of Patent: December 9, 1980Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, Robert J. Carrico
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Patent number: 4230797Abstract: An improved heterogeneous specific binding assay method which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium. The method is carried out using reagent means which comprises, as its labeled constituent, a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction system is utilized as means for monitoring the extent of binding of the labeled constituent in conventional heterogeneous specific binding assay schemes. The presence of a ligand in a liquid medium may be determined following conventional competitive binding manipulative techniques.Type: GrantFiled: April 10, 1978Date of Patent: October 28, 1980Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, Robert J. Carrico, James E. Christner
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Patent number: 4226978Abstract: .beta.-Galactosyl-umbelliferone labeled aminoglycoside antibiotics, e.g., gentamicin, useful in homogeneous and heterogeneous specific binding assays for such antibiotics in liquid media such as serum. Also provided are a .beta.-galactosyl-umbelliferone-carboxylic acid and salts thereof which are intermediates in a method of preparing the labeled antibiotics.Type: GrantFiled: March 13, 1978Date of Patent: October 7, 1980Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, Robert J. Carrico, John F. Burd
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Patent number: 4134792Abstract: A specific binding assay method employing, as a labeling substance, a reversibly binding enzyme modulator for the detection of a ligand in a liquid medium. The method follows conventional specific binding assay techniques of either the homogeneous or heterogeneous type wherein the liquid medium to be assayed is combined with reagent means that includes a labeled conjugate to form a binding reaction system having a bound-species and a free-species of the conjugate. The amount of conjugate resulting in the bound-species or the free-species is a function of the amount of ligand present in the liquid medium assayed. In the present invention, the labeled conjugate comprises a reversibly binding enzyme modulator covalently linked to a binding component of the binding reaction system.Type: GrantFiled: December 6, 1976Date of Patent: January 16, 1979Assignee: Miles Laboratories, Inc.Inventors: Robert C. Boguslaski, Robert J. Carrico, James E. Christner