Abstract: A method of decellularizing a tissue includes disposing the tissue within a device, the device. The device includes a container, first and second electrodes disposed within the container and defining a space between the first and second electrodes to receive the tissue, a perfusion pump, and a conduit connected to the perfusion pump to transport a decellularization composition from the pump into the tissue. The method further includes disposing the decellularization composition within the container to surround the tissue and contact the first and second electrodes, applying an electric potential across the first and second electrodes, and perfusing the decullarization composition into the tissue.

Abstract: A method of decellularizing a tissue includes disposing the tissue within a device, the device. The device includes a container, first and second electrodes disposed within the container and defining a space between the first and second electrodes to receive the tissue, a perfusion pump, and a conduit connected to the perfusion pump to transport a decellularization composition from the pump into the tissue. The method further includes disposing the decellularization composition within the container to surround the tissue and contact the first and second electrodes, applying an electric potential across the first and second electrodes, and perfusing the decullarization composition into the tissue.

Abstract: An analyte detection system includes a detector situated close to a well of a substrate. The well includes conjugated paramagnetic beads. The detection system also includes a magnetic field generator that provides an oscillating magnetic field in the well and the detector, an oscillator circuit coupled to the detector, and a circuit coupled to the detector that detect the conjugated paramagnetic beads. A method includes applying a magnetic field to well of a substrate with conjugated paramagnetic beads, alternating the polarity of the magnetic field, detecting a waveform associated with the alternating magnetic field, and associating the waveform with the quantity of conjugated paramagnetic beads. An analyte detection kit includes a substrate with an attached antibody that is reactive to the analyte, a conjugated paramagnetic particle, and a conjugated paramagnetic particle detector.

Abstract: A device for decellularizing a tissue includes a container, first and second electrodes disposed within the container and defining a space between the first and second electrodes to receive the tissue, a perfusion pump, and a conduit connected to the perfusion pump to transport a decellularization solution from the pump into the tissue.

Abstract: The disclosure is directed to a transcranial Doppler probe. The transcranial Doppler probe includes a spherical bearing, a piezoelectric transducer pivotally attached to the spherical bearing, and first and second rods coupled to the piezoelectric transducer. The first rod is configured to pivot the piezoelectric transducer around a first pivot axis and the second rod is configured to pivot the piezoelectric transducer around a second pivot axis.

Abstract: The disclosure is directed to a transcranial Doppler probe. The transcranial Doppler probe includes a spherical bearing, a piezoelectric transducer pivotally attached to the spherical bearing, and first and second rods coupled to the piezoelectric transducer. The first rod is configured to pivot the piezoelectric transducer around a first pivot axis and the second rod is configured to pivot the piezoelectric transducer around a second pivot axis.

Abstract: A method of detecting a target substance includes contacting the target substance with a substrate. The substrate has a first receptor bound to the substrate. The target substance binds to the first receptor. The method further includes contacting a second receptor with the substrate. The second receptor is associated with the target substance. The second receptor is biotinylated. The method also includes contacting an anti-biotin antibody conjugated paramagnetic particle with the substrate.

Abstract: An analyte detection system includes a detector situated close to a well of a substrate. The well includes conjugated paramagnetic beads. The detection system also includes a magnetic field generator that provides an oscillating magnetic field in the well and the detector, an oscillator circuit coupled to the detector, and a circuit coupled to the detector that detect the conjugated paramagnetic beads. A method includes applying a magnetic field to well of a substrate with conjugated paramagnetic beads, alternating the polarity of the magnetic field, detecting a waveform associated with the alternating magnetic field, and associating the waveform with the quantity of conjugated paramagnetic beads. An analyte detection kit includes a substrate with an attached antibody that is reactive to the analyte, a conjugated paramagnetic particle, and a conjugated paramagnetic particle detector.

Abstract: A method of detecting a target substance includes contacting the target substance with a substrate. The substrate has a first receptor bound to the substrate. The target substance binds to the first receptor. The method further includes contacting a second receptor with the substrate. The second receptor is associated with the target substance. The second receptor is biotinylated. The method also includes contacting an anti-biotin antibody conjugated paramagnetic particle with the substrate.

Abstract: There is a tremendous need for high throughput gene expression technology which can efficiently and cheaply identify and accurately isolate different genes expressed between diseased and normal tissues for use in discovering new drugs. The present invention utilizes a combination of biomolecular chemistry methods to eliminate/degrade redundant sequences and fluorescence dye assay to identify these unique sequences from two cell or tissue populations. cDNA from normal or diseased cells or tissues are hybridized with the RNA of the complement normal or diseased cells or tissues. The hybridized cDNA/RNA is incubated with exonucleases, resulting in degradation of all but the single stranded RNA and DNA. RNA are then elimated using RNase and the remaining DNA which are unique to the sample are amplified.

Abstract: There is a tremendous need for high throughput gene expression technology which can efficiently and cheaply identify and accurately isolate different genes expressed between diseased and normal tissues for use in discovering new drugs. The present invention utilizes a combination of biomolecular chemistry methods to eliminate/degrade redundant sequences and fluorescence dye assay to identify these unique sequences from two cell or tissue populations. cDNA from normal or diseased cells or tissues are hybridized with the RNA of the complement normal or diseased cells or tissues. The hybridized cDNA/RNA is incubated with exonucleases, resulting in degradation of all but the single stranded RNA and DNA. RNA are then elimated using RNase and the remaining DNA which are unique to the sample are amplified.

Abstract: The present invention additionally comprises a method and apparatus for generating and mixing signals for frequency-domain lifetime and spectral fluorometry. The present invention comprises a plurality of signal generators that generate a plurality of signals where the signal generators modulate the amplitude and/or the frequency of the signals. The present invention uses one of these signals to drive an excitation signal that the present invention then directs and transmits at a target mixture, which absorbs the energy from the excitation signal. The property of fluorescence causes the target mixture to emit an emitted signal that the present invention detects with a signal detector. The present invention uses a plurality of mixers to produce a processor reference signal and a data signal. The present invention then uses a processor to compare the processor reference signal with the data signal by analyzing the differences in the phase and the differences in the amplitude between the two signals.

Abstract: The present invention is a system for chemometric analysis for the extraction of the individual component fluorescence spectra and fluorescence lifetimes from a target mixture. The present invention combines a processor with an apparatus for generating an excitation signal to transmit at a target mixture and an apparatus for detecting the emitted signal from the target mixture. The present invention extracts the individual fluorescence spectrum and fluorescence lifetime measurements from the frequency and wavelength data acquired from the emitted signal. The present invention uses an iterative solution that first requires the initialization of several decision variables and the initial approximation determinations of intermediate matrices. The iterative solution compares the decision variables for convergence to see if further approximation determinations are necessary.

Abstract: Apparatus and method for measuring the relative distance between different areas on a remote surface or displacements of a selected area of a remote surface with respect to a measuring apparatus are provided. A laser beam is modulated at an RF frequency and focused on a selected area. The selected area is scanned by movement of a mirror and at each selected area the phase difference is measured. Digital data processing of signals derived from the source and return beams is used to provide high precision. For detecting or measuring vibration or displacement, an analog signal is obtained from a low-pass filter.

Abstract: The present invention additionally comprises a method and apparatus for generating and mixing signals for frequency-domain lifetime and spectral fluorometry. The present invention comprises a plurality of signal generators that generate a plurality of signals where the signal generators modulate the amplitude and/or the frequency of the signals. The present invention uses one of these signals to drive an excitation signal that the present invention then directs and transmits at a target mixture, which absorbs the energy from the excitation signal. The property of fluorescence causes the target mixture to emit an emitted signal that the present invention detects with a signal detector. The present invention uses a plurality of mixers to produce a processor reference signal and a data signal. The present invention then uses a processor to compare the processor reference signal with the data signal by analyzing the differences in the phase and the differences in the amplitude between the two signals.