Abstract: A method and system to deposit rapidly, accurately and efficiently liquid droplets onto a sample plate. A liquid from which the droplet is formed contains sample molecules, matrix molecules or a mixture of both sample and matrix molecules. A sample plate is positioned with respect to a position below a portion of the liquid, such as a droplet. A power supply forms an electric field between the sample plate and the liquid, which pulls the droplet to a target location on the sample plate. A plurality of capillary columns is provided for simultaneous or successive multiple depositions via application of one or more high voltage pulses between the liquid sample array and a sample plate.

Abstract: The invention provides an efficiently automated incubation device that reduces the number of powered moving parts in the device and the amount of air transfer between environments that are internal and external to the device.

Abstract: A method and system to deposit rapidly, accurately and efficiently liquid droplets onto a sample plate. A liquid from which the droplet is formed contains sample molecules, matrix molecules or a mixture of both sample and matrix molecules. A sample plate is positioned with respect to a position below a portion of the liquid, such as a droplet. A power supply forms an electric field between the sample plate and the liquid, which pulls the droplet to a target location on the sample plate. A plurality of capillary columns is provided for simultaneous or successive multiple depositions via application of one or more high voltage pulses between the liquid sample array and a sample plate.

Abstract: The present invention provides highly automated fluid delivery systems and related methods with significantly improved throughput relative to preexisting technologies. In particular, the invention relates to a fluid dispensing system that simultaneously produces multiple fluid mixtures on-the-fly in multiple multiwell plates. The invention also includes control system and machine software in addition to various systems and related methods for performing assorted downstream processes.

Abstract: There is provided a method and apparatus for aspirating a volume of liquid into a syringe from a sample of the liquid. A substantial portion of the liquid in the syringe is subsequently dispensed from the syringe and returned to the sample. Accordingly, a small portion of the aspirated liquid is retained in the syringe. One or more nanoliter volumes of the liquid retained in the syringe are then dispensed from the syringe to a container for further use or analysis. It will be understood, therefore, that the volume of the originally aspirated liquid is substantially larger than the volume of liquid dispensed for further use or analysis. The invention also includes a method and apparatus for aspirating and dispensing a plurality of liquids by using a plurality of syringes, and also can be used to dispense liquids onto microplates.

Abstract: The present invention provides devices, systems, and methods of manifolding or distributing materials to and/or from reaction wells of multiple reaction blocks. Materials are distributed through multiple surfaces of reaction blocks without exposing reaction well contents to external environments. The invention further provides reaction block carriers to array multiple reaction blocks for use in the manifolding devices and systems.

Abstract: This invention provides an apparatus and method for washing a biological chip cartridge. A washing station comprises a cartridge holder that includes at least two cartridge mounting tubes that are structured to retain the biological chip cartridge on the washing station without doors. At least one fluid source communicates with one of the mounting tubes and a waste dump communicates with another mounting tube. A fluid controller controls delivery of a fluid from the fluid source to the mounting tube.

Abstract: The invention provides an efficiently automated incubation device that reduces the number of powered moving parts in the device and the amount of air transfer between environments that are internal and external to the device.

Abstract: The present invention provides highly automated fluid delivery systems and related methods with significantly improved throughput relative to preexisting technologies. In particular, the invention relates to a fluid dispensing system that simultaneously produces multiple fluid mixtures on-the-fly in multiple multiwell plates. The invention also includes control system and machine software in addition to various systems and related methods for performing assorted downstream processes.

Abstract: An automated centrifuge comprising a rotor having a plurality of sample receiving elements located in the rotor is provided. Sample processing components are structured to be insertable into any one of the receiving elements and a controller is configured to insert the sample processing components into the sample receiving elements. The sample receiving elements located in the rotor are grouped in clusters, and the cavities of each cluster are substantially parallel. Also, an automated centrifuge system comprising a rotor including a plurality of clusters of receiving elements, each element including a longitudinal axis, with the longitudinal axes of each element in a cluster being substantially parallel is provided. A plurality of sample processing components are arranged in groups, with each group configured to be received into adjacent clusters. A rotor position member is structured to determine the position of each cluster.

Abstract: A method and system to deposit rapidly, accurately and efficiently liquid droplets onto a sample plate. A liquid from which the droplet is formed contains sample molecules, matrix molecules or a mixture of both sample and matrix molecules. A sample plate is positioned with respect to a position below a portion of the liquid, such as a droplet. A power supply forms an electric field between the sample plate and the liquid, which pulls the droplet to a target location on the sample plate. A plurality of capillary columns is provided for simultaneous or successive multiple depositions via application of one or more high voltage pulses between the liquid sample array and a sample plate.

Abstract: This invention provides an object holder for precisely positioning an object such as a microtiter plate on a support fixture. The object holders can also include a retaining device on a support fixture for receiving an object. In use, the object is generally positioned on the fixture relative to alignment surfaces of the object. Pushers then precisely position the object in a desired location. The invention also provides integrated systems that coordinate the actions of different components of the object holders. For example, once an object is in a desired position, a controller can activate a retaining device to retain the object in the object holder in the desired orientation.

Abstract: A fuel management control method for a hybrid electric vehicle drive having an internal combustion engine and an electric motor arranged in parallel such that both can propel the vehicle; the system including an electric motor driven fuel pump and a programmable microprocessor; and wherein the method further includes monitoring vehicle speed and sensing braking pressure and directing signals of both vehicle speed and braking to the microprocessor and processing such inputs in accordance with an aggressive fuel management program including shut-off of fuel flow to the gas engine in response to vehicle braking at vehicle speeds above a predetermined maximum hysteresis speed and maintaining the fuel shut off during vehicle coasting above a predetermined speed while controlling the electric motor to provide regenerative braking or vehicle start during such fuel shut off modes of operation.

Abstract: A five-speed parallel-shaft clutch-to-clutch shifting automatic transmission has an hydraulically responsive clutch priority valve effective to exhaust fluid pressure from lower priority clutches upon undesirable simultaneous application two or more clutches. Two clutches associated with numerically nonadjacent gear ratios are supplied fluid pressure from a single solenoid controlled fluid valve vis-a-vis a two mutually exclusive state multiplex valve. The clutch priority valve is interposed between the solenoid controlled fluid valve and the multi-plex valve thereby rendering the two clutch states associated with the single solenoid valve indistinguishable by the clutch priority valve.

Abstract: A power transmission has three axes on which rotatably supported gears and selectively operable clutches are disposed to provide five forward speed ratios. The two highest speed ratios (fourth and fifth) have identical input clutch and gear units. The fourth ratio, the torque transmission path, includes a transfer gear ratio and utilizes the second speed ratio gears to develop a speed ratio different from the fifth speed ratio.

Abstract: A countershaft type power transmission has a plurality of selectively engageable friction clutches for establishing a plurality of forward drive ratios and a reverse drive ratio between meshing gear members on an input shaft, a countershaft and an output shaft. One of the power paths established by the meshing gears includes a gear member rotationally fast on the output shaft. A parking pawl member is selectively engageable with this gear member to prevent output shaft rotation when parking is desired. The pawl has teeth formed thereon having a profile which permit continuous simultaneous engagement between two teeth on the pawl and two teeth on the gear member regardless of the rotational bias imposed on the output shaft during parking.

Abstract: A lubrication system for multi-plate clutch assemblies is controlled to provide increased lube flow. During steady state operation, lube flow is increased periodically for a short time interval. At other times during steady state operation, the lube flow is maintained at a level requiring minimum fluid flow for the system.

Abstract: A power transmission has an input shaft, an output shaft and a transfer or secondary input shaft disposed on parallel noncoaxial axes. A transfer gear train including a gear member supported on each axis is connectible to the input shaft through a forward friction clutch for drivingly connecting the input shaft and the transfer shaft through a one-way drive connection to the output shaft. The transfer gear train provides the first and lowest forward drive ratio between the input and output shafts through the one-way drive connection. A plurality of friction clutches on the input and transfer shafts and associated reaction gears on the shafts are selectively operable to provide a plurality of higher forward ratios during which the one-way device permits the output shaft to overcome the transfer gear mounted thereon.

Abstract: A mechanical clutch is provided to selectively interconnect a forward or reverse gear member to a transmission shaft. The clutch is operated by a servo piston and fork structure. The servo piston is slidably disposed in a housing and urged in the forward select position by a spring. The housing and servo piston cooperate to define two fluid chambers which are maintained in a separated condition when the forward select position is established. One chamber is in fluid communication with a fluid system that is controlled to provide fluid at two distinct pressure levels, depending upon various vehicle operating parameters. The other chamber is connected with a selectively engageable fluid operated friction clutch which is effective to assist in establishing at least the reverse drive ratio within the transmission. The lowest level of fluid pressure in the one chamber is not sufficient to cause the piston to overcome the spring force and thereby move the clutch to the reverse select position.

Abstract: A hydraulic transmission control uses a plurality of solenoid valves to control the engagement of friction devices during the shifting of a transmission between gear ratios. A clutch priority valve is provided to prevent the continuous simultaneous engagement of friction devices which will establish more than one ratio. Also provided is a manually controlled valve which permits the operator to determine the range in which the transmission will operate.If the clutch priority valve has been actuated, the transmission ratio can be controlled between a low ratio, for example, second gear, and a high ratio, for example, third or fourth gear, by use of the manual valve in combination with an actuator mode valve. The clutch priority valve, when actuated, is also operable to disengage a torque converter clutch.