Abstract: Provided herein are methods for identifying centromeres and centromeres identified by such methods. Centromeres of organisms such as algae, fungi, and protists can be used, for example, for constructing artificial chromosomes and cells containing such artificial chromosomes.

Abstract: The invention relates to methods for producing lipids such as fatty acid products in recombinant host cells engineered to express a non-native gene encoding a dehydrogenase. The recombinant microorganisms are able to proliferate at a higher rate as compared with microorganisms that do not express a non-native dehydrogenase gene, and cultures of microorganisms that are engineered for lipid production and that express a non-native dehydrogenase produce more lipid than cultures of control microorganisms that do not include a non-native dehydrogenase gene.

Abstract: The invention provides transgenic photosynthetic microorganisms that include non-native genes encoding polypeptides having lipolytic activity for production of free fatty acids and fatty acid derivatives, and methods of producing free fatty acids and fatty acid derivatives using the transgenic microorganisms disclosed herein. The invention also provides transgenic microorganisms that include non-native genes encoding polypeptides having lipolytic activity, and novel genes encoding polypeptides demonstrating lipolytic activity.

Abstract: The present invention provides for the improved production of fatty acid products in a recombinant microorganism or host cell engineered to express a non-native gene encoding a thioesterase and a non-native gene encoding a lysophosphatidic acid acyltransferase (LPAAT), in which the LPAAT is overexpressed in the recombinant microorganism compared to an otherwise identical microorganism that does not include the non-native LPAAT gene. The invention also provides methods of producing fatty acid products using such recombinant microorganisms.

Abstract: The described invention provides genetically engineered microorganisms, including photosynthetic microorganisms, expressing 4-hydroxybenzoyl-CoA thioesterases and methods of using the genetically engineered microorganisms for producing free fatty acids and/or fatty acid derivatives.

Abstract: The invention relates to methods for producing lipids such as fatty acid products in recombinant host cells engineered to express a non-native gene encoding a dehydrogenase. The recombinant microorganisms are able to proliferate at a higher rate as compared with microorganisms that do not express a non-native dehydrogenase gene, and cultures of microorganisms that are engineered for lipid production and that express a non-native dehydrogenase produce more lipid than cultures of control microorganisms that do not include a non-native dehydrogenase gene.

Abstract: The present invention provides for the improved production of fatty acid products in a recombinant microorganism or host cell engineered to express a non-native gene encoding a thioesterase and a non-native gene encoding a lysophosphatidic acid acyltransferase (LPAAT), in which the LPAAT is overexpressed in the recombinant microorganism compared to an otherwise identical microorganism that does not include the non-native LPAAT gene. The invention also provides methods of producing fatty acid products using such recombinant microorganisms.

Abstract: The invention provides transgenic photosynthetic microorganisms that include non-native genes encoding polypeptides having lipolytic activity for production of free fatty acids and fatty acid derivatives, and methods of producing free fatty acids and fatty acid derivatives using the transgenic microorganisms disclosed herein. The invention also provides transgenic microorganisms that include non-native genes encoding polypeptides having lipolytic activity, and novel genes encoding polypeptides demonstrating lipolytic activity.

Abstract: The described invention provides genetically engineered microorganisms, including photosynthetic microorganisms, expressing 4-hydroxybenzoyl-CoA thioesterases and methods of using the genetically engineered microorganisms for producing free fatty acids and/or fatty acid derivatives.

Abstract: Provided herein are methods for identifying centromeres and centromeres identified by such methods. Centromeres of organisms such as algae, fungi, and protists can be used, for example, for constructing artificial chromosomes and cells containing such artificial chromosomes.

Abstract: This invention describes genes, metabolic pathways, microbial strains and methods to produce methyl butanol and other compounds of interest from renewable feedstocks.

Abstract: A lactamase enzyme having good stability, capable of hydrolysing an enantiomer of the bicyclic lactam, 2-azabicyclo[2.2.1]hept-5-en-3-one, to give (−) lactam and (+) amino acid, has been found in a strain of Comamonas acidivorans. The enzyme has been isolated and cloned, and its structure identified.

Abstract: A lactamase enzyme having good stability, capable of hydrolysing an enatiomer of the bicyclic lactam, 2-azabicyclo[2.2.1]hept-5-en-3-one, to give (-) lactam and (+) amino acid, has been found in a strain of Comamonas acidivorans. The enzyme has been isolated and cloned, and its structure identified.