Abstract: The present document describes a dual-affinity probe comprising an organic or inorganic surface binding peptide and a target-specific capture element, which may bind to various targets, such as pathogens. This document further describes uses of the dual-affinity probe, and kits to determine the presence of and/or quantity of a target in a sample. In particular embodiments, the dual-affinity probe is specific for SARS-CoV-2 (Spike or Nucleocapsid) protein and may be used to determine whether a subject is infected with SARS-CoV-2.

Abstract: The present document describes a dual-affinity probe comprising an inorganic surface binding peptide and a target-specific capture element, which may bind to various targets, such as pathogens. This document further describes uses of the dual-affinity probe, e.g., to determine the presence of and/or quantity of a target in a sample. In particular embodiments, the dual-affinity probe is specific for SARS-CoV-2 (Spike or Nucleocapsid) protein and may be used to determine whether a subject is infected with SARS-CoV-2.

Abstract: The disclosure provides a magnetic reagent comprised of a recombinant yeast cell having the following genetic modifications: impairment of the CCC1 gene; addition of at least one copy of a human ferritin gene complex; addition of at least one copy of a TCO89 gene; and addition of at least one copy of a mineral- or metal ion-adsorbing target peptide, wherein the magnetic susceptibility or mass magnetization of said magnetic reagent is greater than it would be for a native yeast.