Patents by Inventor Robert D. Bremel
Robert D. Bremel has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20160132631Abstract: This invention relates to the identification of peptide binding to ligands, and in particular to identification of epitopes expressed by microorganisms and by mammalian cells. The present invention provides polypeptides comprising the epitopes, and vaccines, antibodies and diagnostic products that utilize or are developed using the epitopes.Type: ApplicationFiled: June 9, 2014Publication date: May 12, 2016Inventors: Robert D. Bremel, Jane Homan
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Publication number: 20160117441Abstract: The present invention provides a bioinformatic methodology for prediction of peptidase cleavage sites based on principal component analysis and based on training sets obtained by experimental protein cleavage. This invention is not limited to training sets derived from CSL approaches, nor to any other experimental determination of cleavage site. Undoubtedly there will be new approaches to developed for experimental measurement of cleavage sites and these too may be the source of training sources for the present invention.Type: ApplicationFiled: June 9, 2014Publication date: April 28, 2016Inventor: Robert D. Bremel
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Patent number: 8703134Abstract: The present invention relates to fusion proteins comprising a microorganism targeting molecule (e.g., immunoglobulin) and a biocide. The present invention also relates to therapeutic and prophylactic methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in diverse fields.Type: GrantFiled: February 6, 2013Date of Patent: April 22, 2014Assignees: ioGenetics, LLC, Arizona Board of Regents on Behalf of the University of ArizonaInventors: Michael Imboden, Michael Riggs, Deborah A. Schaefer, Jane Homan, Robert D. Bremel
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Publication number: 20130330335Abstract: This invention relates to the identification of peptide binding to ligands, and in particular to identification of epitopes expressed by microorganisms and by mammalian cells. The present invention provides polypeptides comprising the epitopes, and vaccines, antibodies and diagnostic products that utilize or are developed using the epitopes.Type: ApplicationFiled: March 21, 2011Publication date: December 12, 2013Applicant: IOGENETICS, LLCInventors: Robert D. Bremel, Jane Homan
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Publication number: 20130230516Abstract: The present invention relates to fusion proteins comprising a microorganism targeting molecule (e.g., immunoglobulin) and a biocide. The present invention also relates to therapeutic and prophylactic methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in diverse fields.Type: ApplicationFiled: February 6, 2013Publication date: September 5, 2013Applicants: Arizona Board of Regents on behalf of the University of Arizona, ioGenetics, LLCInventors: Michael Imboden, Michael Riggs, Deborah A. Schaefer, Jane Homan, Robert D. Bremel
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Patent number: 8222188Abstract: The present invention relates to the production of antibody libraries. In particular, the present invention relates to the use of integrating retroviral vectors to generate libraries comprising a plurality of combinations of antibody light chains and heavy chains. The present invention thus provides improved methods of generating and screening antibody libraries comprising large numbers of unique antibodies.Type: GrantFiled: June 29, 2009Date of Patent: July 17, 2012Assignee: Catalent Pharma Solutions, LLCInventors: Robert D. Bremel, Kurt Eakle, Michael Imboden
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Publication number: 20100111976Abstract: The present invention relates to retroviral constructs that encode novel monoclonal antibodies, novel fusion proteins, and chimeric monoclonal antibodies and to methods of using and producing the same. In particular, the present invention relates to methods of producing a fusion protein comprising a microorganism targeting molecule (e.g., immunoglobulin or innate immune system receptor molecule) and a biocide (e.g., bactericidal enzymes) in transgenic animals (e.g., bovines) and in cell cultures. The present invention also relates to therapeutic and prophylactic methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in health care (e.g., human and veterinary), agriculture (e.g., animal and plant production), and food processing (e.g., beef carcass processing).Type: ApplicationFiled: September 24, 2009Publication date: May 6, 2010Applicant: IOGENETICS, LLCInventors: Michael Imboden, Jane Homan, Robert D. Bremel
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Publication number: 20100009869Abstract: The present invention relates to the production of antibody libraries. In particular, the present invention relates to the use of integrating retroviral vectors to generate libraries comprising a plurality of combinations of antibody light chains and heavy chains. The present invention thus provides improved methods of generating and screening antibody libraries comprising large numbers of unique antibodies.Type: ApplicationFiled: June 29, 2009Publication date: January 14, 2010Inventors: Robert D. Bremel, Kurt Eakle, Michael Imboden
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Publication number: 20090098121Abstract: The present invention relates to retroviral constructs that encode novel monoclonal antibodies, novel fusion proteins, and chimeric monoclonal antibodies and to methods of using and producing the same. In particular, the present invention relates to methods of producing a fusion protein comprising a microorganism targeting molecule (e.g., immunoglobulin or innate immune system receptor molecule) and a biocide (e.g., bactericidal enzymes) in transgenic animals (e.g., bovines) and in cell cultures. The present invention also relates to therapeutic and prophylactic methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in health care (e.g., human and veterinary), agriculture (e.g., animal and plant production), and food processing (e.g., beef carcass processing).Type: ApplicationFiled: October 19, 2007Publication date: April 16, 2009Applicant: ioGenetics, LLCInventors: Michael Imboden, Jane Homan, Robert D. Bremel
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Publication number: 20080267943Abstract: The present invention relates to retroviral constructs that encode novel monoclonal antibodies, novel fusion proteins, and chimeric monoclonal antibodies and to methods of using and producing the same. In particular, the present invention relates to methods of producing a fusion protein comprising a microorganism targeting molecule (e.g., immunoglobulin or innate immune system receptor molecule) and a biocide (e.g., bactericidal enzymes) in transgenic animals (e.g., bovines) and in cell cultures. The present invention also relates to therapeutic and prophylactic methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in health care (e.g., human and veterinary), agriculture (e.g., animal and plant production), and food processing (e.g., beef carcass processing).Type: ApplicationFiled: October 19, 2007Publication date: October 30, 2008Applicant: ioGenetics, LLCInventors: Michael Imboden, Jane Homan, Robert D. Bremel
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Publication number: 20080269122Abstract: The present invention relates to retroviral constructs that encode novel monoclonal antibodies, novel fusion proteins, and chimeric monoclonal antibodies and to methods of using and producing the same. In particular, the present invention relates to methods of producing a fusion protein comprising a microorganism targeting molecule (e.g., immunoglobulin or innate immune system receptor molecule) and a biocide (e.g., bactericidal enzymes) in transgenic animals (e.g., bovines) and in cell cultures. The present invention also relates to therapeutic and prophylactic methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in health care (e.g., human and veterinary), agriculture (e.g., animal and plant production), and food processing (e.g., beef carcass processing).Type: ApplicationFiled: October 19, 2007Publication date: October 30, 2008Applicant: ioGenetics, LLCInventors: Michael Imboden, Jane Homan, Robert D. Bremel
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Patent number: 7384738Abstract: The present invention relates to the expression and screening of genomic DNA sequences encoding uncharacterized genes and proteins. The present invention provides systems utilizing unique features of retroviral replication to analyze uncharacterized genes derived from genomic DNA samples. In preferred embodiments, a segment of genomic DNA is inserted between 5? and 3? viral long terminal repeats (LTRs) in a vector (e.g., a plasmid, cosmid, or artificial chromosome vector). The resulting vector (or library of vectors containing a plurality of independent genomic sequences) is then introduced into a retroviral packaging cell. The resulting provirus or proteins expression from the provirus are then analyzed.Type: GrantFiled: March 26, 2003Date of Patent: June 10, 2008Inventors: Robert D. Bremel, Gregory T. Bleck, Michael Imboden, Kurt Eakle
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Patent number: 7332333Abstract: The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.Type: GrantFiled: December 21, 2004Date of Patent: February 19, 2008Assignee: Gala Design, Inc.Inventors: Robert D. Bremel, Linda U. Miller, Gregory T. Bleck
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Patent number: 7300790Abstract: The present invention relates to non-vaccinal and non-pharmacologic compositions and methods for controlling complex retroviral infections. In particular, the present invention provides transgenic animals expressing a transdominant negative Rex gene product that inhibits retroviral replication.Type: GrantFiled: January 23, 2004Date of Patent: November 27, 2007Assignee: ioGenetics, LLCInventors: Kurt Eakle, Thomas Hope, Eun-A Choi, Jane Homan, Robert D. Bremel
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Patent number: 7297536Abstract: Inducible gene expression systems and a method thereof. A first inducible gene expression system includes a first vector comprising at least one retroviral promoter and at least one factor to induce the retroviral promoter. At least one gene product is expressed in proportion to retroviral promoter induction. The method includes providing a first vector comprising at least one retroviral promoter and providing at least one factor corresponding to the retroviral promoter. The retroviral promoter is induced with the at least one factor. At least one protein is expressed based on the induction of the retroviral promoter. A second inducing expression system includes a first vector comprising at least one retroviral promoter, an inducer for the retroviral promoter, and at least one protein expressed in proportion to retroviral promoter induction.Type: GrantFiled: January 23, 2004Date of Patent: November 20, 2007Assignee: Wisconsin Alumni Research FoundationInventors: Jerome S. Harms, Gary A. Splitter, Kurt A. Eakle, Robert D. Bremel
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Patent number: 7138562Abstract: The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.Type: GrantFiled: July 18, 2001Date of Patent: November 21, 2006Assignee: Wisconsin Alumni research FoundationInventors: Robert D. Bremel, Anthony W. S. Chan, Jane C. Burns
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Patent number: 6852510Abstract: The present invention relates to the production of proteins in host cells, and, more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.Type: GrantFiled: June 29, 2001Date of Patent: February 8, 2005Assignee: Gala Design IncInventors: Robert D. Bremel, Linda U. Miller, Gregory T. Bleck
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Publication number: 20040253581Abstract: The present invention relates to non-vaccinal and non-pharmacologic compositions and methods for controlling complex retroviral infections. In particular, the present invention provides transgenic animals expressing a transdominant negative Rex gene product that inhibits retroviral replication.Type: ApplicationFiled: January 23, 2004Publication date: December 16, 2004Applicant: Gala Design, Inc.Inventors: Kurt Eakle, Thomas Hope, Eun-A Choi, Jane Homan, Robert D. Bremel
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Publication number: 20040235173Abstract: The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector comprising an exogenous gene and methods of making such host cells by serial transduction or transfection. The present invention further provides methods of expressing increased levels of protein in host cells using such vectors.Type: ApplicationFiled: January 16, 2004Publication date: November 25, 2004Applicant: Gala Design, Inc.Inventors: Gregory T. Bleck, Robert D. Bremel, Linda U. Miller
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Publication number: 20040038304Abstract: The present invention relates to the production of antibody libraries. In particular, the present invention relates to the use of integrating retroviral vectors to generate libraries comprising a plurality of combinations of antibody light chains and heavy chains. The present invention thus provides improved methods of generating and screening antibody libraries comprising large numbers of unique antibodies.Type: ApplicationFiled: March 27, 2003Publication date: February 26, 2004Applicant: Gala Design, Inc.Inventors: Robert D. Bremel, Kurt Eakle, Michael Imboden