Patents by Inventor Robert Dicosimo

Robert Dicosimo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Perhydrolase providing improved specific activity
    Patent number: 8389254
    Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
    Type: Grant
    Filed: March 23, 2011
    Date of Patent: March 5, 2013
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Robert Dicosimo, John Edward Gavagan, Mark Scott Payne
  • Perhydrolase providing improved specific activity
    Patent number: 8389255
    Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
    Type: Grant
    Filed: March 23, 2011
    Date of Patent: March 5, 2013
    Assignee: E.I. de Pont de Nemours and Company
    Inventors: Robert Dicosimo, John Edward Gavagan, Mark Scott Payne
  • Perhydrolase variant providing improved specific activity
    Patent number: 8389260
    Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritime acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
    Type: Grant
    Filed: December 16, 2011
    Date of Patent: March 5, 2013
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Mark Scott Payne, John Edward Gavagan
  • Control of enzymatic peracid generation
    Patent number: 8389575
    Abstract: A process is provided for producing target concentrations of peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity under conditions where control of reaction pH by selection of buffer concentration and concentration of perhydrolase and reactants produces a targeted concentration of peroxycarboxylic acids. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided, as are corresponding methods of use.
    Type: Grant
    Filed: January 20, 2012
    Date of Patent: March 5, 2013
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Mark Scott Payne, Eugenia Costa Hann
  • Perhydrolase variant providing improved specific activity
    Patent number: 8389258
    Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
    Type: Grant
    Filed: December 16, 2011
    Date of Patent: March 5, 2013
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Mark Scott Payne, John Edward Gavagan
  • Perhydrolase variant providing improved specific activity
    Patent number: 8389256
    Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
    Type: Grant
    Filed: December 16, 2011
    Date of Patent: March 5, 2013
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Robert Dicosimo, Mark Scott Payne, John Edward Gavagan
  • PRODUCTION OF ALCOHOL ESTERS IN SITU USING ALCOHOLS AND FATTY ACIDS PRODUCED BY MICROORGANISMS
    Publication number: 20130040340
    Abstract: Fatty acid alcohol esters that may be used as biodiesel are synthesized from microbially produced alcohol and fatty acids using an in situ process. The process utilizes a catalyst capable of esterifying the alcohol and fatty acid products of microorganisms, which are produced from renewable resources.
    Type: Application
    Filed: February 6, 2012
    Publication date: February 14, 2013
    Applicant: E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Michael Dauner, Robert Dicosimo, Ranjan Patnaik
  • Stabilization of perhydrolases
    Patent number: 8367597
    Abstract: Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
    Type: Grant
    Filed: August 19, 2011
    Date of Patent: February 5, 2013
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert Dicosimo, Arie Ben-Bassat, Mark S. Payne, Raymond Richard Zolandz
  • Production of peracids using an enzyme having perhydrolysis activity
    Patent number: 8367728
    Abstract: A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.
    Type: Grant
    Filed: March 11, 2010
    Date of Patent: February 5, 2013
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, John E. Gavagan, Mark Payne, Frederick B. Cooling, III
  • ENZYMATIC PERACID GENERATION FORMULATION
    Publication number: 20130004479
    Abstract: Disclosed herein are multi-component formulations for enzymatically producing aqueous solutions of peroxycarboxylic acids suitable for use in, e.g., disinfectant and/or bleaching applications. The multi-component peroxycarboxylic acid formulations comprise at least one carbohydrate esterase family 7 enzyme having perhydrolytic activity.
    Type: Application
    Filed: September 11, 2012
    Publication date: January 3, 2013
    Inventors: Robert Dicosimo, Arie Ben-Bassat, William R. Cahill, David George Dipietro, Eugenia Costa Hann, Mark S. Payne, Richard Alan Reynolds, Raymond Richard Zolandz
  • ENZYMATIC PERACID GENERATION FOR USE IN ORAL CARE PRODUCTS
    Publication number: 20120328534
    Abstract: Disclosed herein are compositions and methods to treat an oral cavity surface with a peracid-based benefit agent. The peracid benefit agent can be use for oral surface bleaching, whitening, disinfecting, destaining, deodorizing, decreasing or removing biofilm, and combinations thereof. The peracid is enzymatically generated from a carboxylic acid ester substrate using a CE-7 carbohydrate esterase having perhydrolytic activity (perhydrolase) in the presence of a source of peroxygen. A fusion protein comprising the perhydrolase coupled to a peptidic component having affinity for an oral cavity surface, either directly or through an optional linker, may be used to target the perhydrolytic activity to the oral cavity surface.
    Type: Application
    Filed: December 19, 2011
    Publication date: December 27, 2012
    Applicant: E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Lisa A. Butterick, Scott D. Cunningham, Robert DiCosimo, Kari A. Fosser, Tanja Maria Gruber, Sharon L. Haynie, Mark S. Payne, Pierre E. Rouviere, Hong Wang
  • Multi-component peracid generation system
    Patent number: 8337905
    Abstract: Disclosed herein are multi-component peroxycarboxylic acid generation systems for enzymatically producing aqueous formulations of peroxycarboxylic acids suitable for use in, e.g., disinfectant and/or bleaching applications. The multi-component peroxycarboxylic acid generation systems comprise at least one carbohydrate esterase family 7 enzyme having perhydrolytic activity.
    Type: Grant
    Filed: October 1, 2009
    Date of Patent: December 25, 2012
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert Dicosimo, Arie Ben-Bassat, William R. Cahill, David George Dipietro, Eugenia Costa Hann, Mark S. Payne, Richard Alan Reynolds, Raymond Richard Zolandz
  • ENZYMATIC PRODUCTION OF ALCOHOL ESTERS FOR RECOVERY OF DIOLS PRODUCED BY FERMENTATION
    Publication number: 20120322117
    Abstract: Diols produced in fermentation are processed in broth by esterification of the product diol with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product diol, such as 1,3-propanediol, with the carboxylic acid to form the diol esters. The diol esters can be extracted from the broth, and the product diol recovered from the diol esters. The carboxylic acid can also serve as an extractant for removal of the diol esters from the fermentation medium.
    Type: Application
    Filed: December 15, 2011
    Publication date: December 20, 2012
    Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLC
    Inventors: Douglas Robert Anton, Michael Dauner, Robert Dicosimo
  • ENZYMATIC PERACID GENERATION FOR USE IN HAIR CARE PRODUCTS
    Publication number: 20120317733
    Abstract: Disclosed herein are compositions and methods to treat hair with a peracid-based benefit agent. The peracid benefit agent can be used for hair bleaching, hair weakening, hair removal, hair waiving, hair straightening or any combination thereof. The peracid may be enzymatically generated from a carboxylic acid ester substrate using an enzyme having perhydrolytic activity (perhydrolase) in the presence of a source of peroxygen. A fusion protein comprising the perhydrolase coupled to a hair-binding domain, either directly or through an optional linker, may be used to target the perhydrolytic activity to the hair surface.
    Type: Application
    Filed: December 19, 2011
    Publication date: December 20, 2012
    Applicant: E.I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Dexter A. Chisholm, Xiumin Cui, Scott D. Cunningham, Robert DiCosimo, Stephen R. Fahnestock, Tanja Maria Gruber, Yongqing Huang, Xueping Jiang, Anju Parthasarathy, Mark S. Payne, Pierre E. Rouviere, Hong Wang
  • TARGETED PERHYDROLASES
    Publication number: 20120321581
    Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.
    Type: Application
    Filed: December 19, 2011
    Publication date: December 20, 2012
    Applicant: E.I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Robert DiCosimo, Scott D. Cunningham, Stephen R. Fahnestock, Tanja Maria Gruber, Mark S. Payne, Pierre E. Rouviere, Linda Jane Solomon, Hong Wang
  • Perhydrolases for enzymatic peracid generation
    Patent number: 8334120
    Abstract: Disclosed herein are variants enzymes that are structurally classified as CE-7 enzymes and have perhydrolysis activity. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned variant enzymes as well as methods and compositions comprising the variant enzymes. Further, disinfectant formulations comprising the peroxycarboxylic acids produced by the processes described herein are provided.
    Type: Grant
    Filed: September 29, 2011
    Date of Patent: December 18, 2012
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert Dicosimo, Mark S. Payne, Tyler Yin
  • Production of peracids using an enzyme having perhydrolysis activity
    Patent number: 8329441
    Abstract: A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.
    Type: Grant
    Filed: April 15, 2011
    Date of Patent: December 11, 2012
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert Dicosimo, John Edward Gavagan, Mark Scott Payne, Frederick B. Cooling, III
  • Enzymatic peracid production using a cosolvent
    Patent number: 8304218
    Abstract: Disclosed herein are two-component enzymatic peracid generation systems and methods of using such systems wherein the first component comprises a formulation of at least one enzyme catalyst having perhydrolysis activity, a carboxylic acid ester substrate, and a cosolvent and wherein the second component comprises a source of peroxygen in water. The two components are combined to produce an aqueous peracid formulation useful as, e.g., a disinfecting or bleaching agent. Specifically, organic cosolvents are used to control the viscosity of a substrate-containing component and to enhance the solubility of the substrate in an aqueous reaction formulation without causing substantial loss of perhydrolytic activity of the enzyme catalyst.
    Type: Grant
    Filed: December 19, 2011
    Date of Patent: November 6, 2012
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Robert Dicosimo, William R. Cahill, David George Dipietro, Eugenia Costa Hann, Richard Alan Reynolds
  • Stabilization Of Perhydrolases
    Publication number: 20120276609
    Abstract: Disclosed herein are enzyme powders comprising a spray-dried formulation of at least one CE-7 esterase, at least one oligosaccharide excipient, and optionally at least one surfactant. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned enzyme powders. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
    Type: Application
    Filed: July 12, 2012
    Publication date: November 1, 2012
    Inventors: Robert DICOSIMO, Arie Ben-Bassat, Mark S. Payne, Raymond Richard Zolandz
  • Production of peracids using an enzyme having perhydrolysis activity
    Patent number: 8298808
    Abstract: A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.
    Type: Grant
    Filed: July 2, 2009
    Date of Patent: October 30, 2012
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, John Edward Gavagan, Mark Scott Payne, Frederick B. Cooling