Abstract: A method of vacuum sealing a container by drawing a vacuum at the external end of a one way valve configured to automatically close upon a vacuum being achieved in the container. The one way valve utilizing a spherical stopper which randomly reorients at each use to prevent wear incapacitating the valve after repeated uses.

Abstract: A method and device for forcing segregated plasma from a flexible collecting bag containing a layer of plasma over a layer of red blood cells has a member to reduce the chance for a portion of the red blood cells to also flow from the collecting bag. The device has a fixed member mounted normal to a base, and a movable member connected to the base by a hinge and urged toward the fixed member by a spring. The spring squeezes the collecting bag, which is supported between the fixed and movable members, forcing the plasma from the top of the bag. A stop member automatically stops the movement of the movable member when a large portion of the plasma has been forced from the bag. The stop member is releasable, allowing further movement of the movable member to remove the remaining portion of the plasma while being closely monitored by an operator.