Patents by Inventor Robert E. Betzig
Robert E. Betzig has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10721441Abstract: An apparatus includes a light source configured for generating a coherent light beam having a wavelength, ?, a light detector, and beam-forming optics configured for receiving the generated light beam and for generating a plurality of substantially parallel Bessel-like beams directed into a sample in a first direction. Each of the Bessel-like beams has a fixed phase relative to the other Bessel-like beams. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beams and for imaging the received light onto the detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction, where the detector is configured for detecting light received by the imaging optics. A processor configured to generate an image of the sample based on the detected light.Type: GrantFiled: July 24, 2018Date of Patent: July 21, 2020Assignee: Howard Hughes Medical InstituteInventors: Robert E. Betzig, Kai Wang
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Patent number: 10509217Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction. A detector is configured for detecting signal light received by the imaging optics, and an aperture mask is positioned.Type: GrantFiled: December 15, 2017Date of Patent: December 17, 2019Assignee: Howard Hughes Medical InstituteInventor: Robert E. Betzig
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Publication number: 20190199969Abstract: An apparatus includes a light source configured for generating a coherent light beam having a wavelength, ?, a light detector, and beam-forming optics configured for receiving the generated light beam and for generating a plurality of substantially parallel Bessel-like beams directed into a sample in a first direction. Each of the Bessel-like beams has a fixed phase relative to the other Bessel-like beams. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beams and for imaging the received light onto the detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction, where the detector is configured for detecting light received by the imaging optics. A processor configured to generate an image of the sample based on the detected light.Type: ApplicationFiled: July 24, 2018Publication date: June 27, 2019Inventors: Robert E. Betzig, Kai Wang
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Patent number: 10247672Abstract: A method includes: (a) providing spatially-patterned activation radiation to a sample that includes phototransformable labels, where an optical parameter of the spatially-patterned activation radiation varies periodically in space; (b) providing spatially-patterned excitation radiation to the sample, where an optical parameter of the spatially-patterned excitation radiation varies periodically in space, where (a) and (b) create a non-linear fluorescence emission pattern within the sample, the pattern including H modulation harmonics, with H>1. The method includes (c) detecting radiation emitted from the activated and excited labels, (d) storing detected radiation data, and (e) spatially shifting one or both of the spatially-patterned excitation radiation and the spatially-patterned activation radiation with respect to the sample to spatially shift the non-linear fluorescence emission pattern within the sample, and (f) repeating (a)-(e) at least N times, with N>2.Type: GrantFiled: September 29, 2015Date of Patent: April 2, 2019Assignee: Howard Hughes Medical InstituteInventors: Robert E. Betzig, Dong Li
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Patent number: 10051240Abstract: An apparatus includes a light source configured for generating a coherent light beam having a wavelength, ?, a light detector, and beam-forming optics configured for receiving the generated light beam and for generating a plurality of substantially parallel Bessel-like beams directed into a sample in a first direction. Each of the Bessel-like beams has a fixed phase relative to the other Bessel-like beams. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beams and for imaging the received light onto the detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction, where the detector is configured for detecting light received by the imaging optics. A processor configured to generate an image of the sample based on the detected light.Type: GrantFiled: March 15, 2013Date of Patent: August 14, 2018Assignee: Howard Hughes Medical InstituteInventors: Robert E. Betzig, Kai Wang
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Publication number: 20180210184Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction. A detector is configured for detecting signal light received by the imaging optics, and an aperture mask is positioned.Type: ApplicationFiled: December 15, 2017Publication date: July 26, 2018Inventor: Robert E. Betzig
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Patent number: 9891421Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction. A detector is configured for detecting signal light received by the imaging optics, and an aperture mask is positioned.Type: GrantFiled: March 17, 2014Date of Patent: February 13, 2018Assignee: Howard Hughes Medical InstituteInventor: Robert E. Betzig
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Patent number: 9791685Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction. A detector is configured for detecting signal light received by the imaging optics, and an aperture mask is positioned.Type: GrantFiled: March 17, 2014Date of Patent: October 17, 2017Assignee: Howard Hughes Medical InstituteInventor: Robert E. Betzig
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Patent number: 9500846Abstract: Excitation light is focused to a focus within a sample and the focus is scanned within a volume in the sample with scanning optical elements. Signal light emitted from the focus is de-scanned, with the one or more scanning optical elements, onto a wavefront sensor as the focus is scanned within the volume. Based on the descanned signal light, an average aberration created by the volume of the sample of a wavefront of the excitation light is determined. A wavefront of the excitation light is corrected by an amount according to the determined average aberration while the focus is scanned within the volume, the signal light is imaged onto a photosensitive detector as the focus is scanned within the volume, and a wavefront of the imaged signal light is corrected by an amount according to the determined average aberration while the focus is scanned.Type: GrantFiled: March 17, 2015Date of Patent: November 22, 2016Assignee: Howard Hughes Medical InstituteInventors: Robert E. Betzig, Kai Wang
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Patent number: 9477074Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction. A detector is configured for detecting signal light received by the imaging optics, and an aperture mask is positioned.Type: GrantFiled: March 17, 2014Date of Patent: October 25, 2016Assignee: Howard Hughes Medical InstituteInventor: Robert E. Betzig
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Publication number: 20160305883Abstract: A method includes: (a) providing spatially-patterned activation radiation to a sample that includes phototransformable labels, where an optical parameter of the spatially-patterned activation radiation varies periodically in space; (b) providing spatially-patterned excitation radiation to the sample, where an optical parameter of the spatially-patterned excitation radiation varies periodically in space, where (a) and (b) create a non-linear fluorescence emission pattern within the sample, the pattern including H modulation harmonics, with H>1. The method includes (c) detecting radiation emitted from the activated and excited labels, (d) storing detected radiation data, and (e) spatially shifting one or both of the spatially-patterned excitation radiation and the spatially-patterned activation radiation with respect to the sample to spatially shift the non-linear fluorescence emission pattern within the sample, and (f) repeating (a)-(e) at least N times, with N>2.Type: ApplicationFiled: September 29, 2015Publication date: October 20, 2016Inventors: Robert E. Betzig, Dong Li
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Patent number: 9448395Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction. A detector is configured for detecting signal light received by the imaging optics, and an aperture mask is positioned.Type: GrantFiled: March 17, 2014Date of Patent: September 20, 2016Assignee: Howard Hughes Medical InstituteInventor: Robert E. Betzig
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Patent number: 9223125Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction.Type: GrantFiled: March 17, 2014Date of Patent: December 29, 2015Assignee: Howard Hughes Medical InstituteInventor: Robert E. Betzig
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Publication number: 20150362713Abstract: Excitation light is focused to a focus within a sample and the focus is scanned within a volume in the sample with scanning optical elements. Signal light emitted from the focus is de-scanned, with the one or more scanning optical elements, onto a wavefront sensor as the focus is scanned within the volume. Based on the descanned signal light, an average aberration created by the volume of the sample of a wavefront of the excitation light is determined. A wavefront of the excitation light is corrected by an amount according to the determined average aberration while the focus is scanned within the volume, the signal light is imaged onto a photosensitive detector as the focus is scanned within the volume, and a wavefront of the imaged signal light is corrected by an amount according to the determined average aberration while the focus is scanned.Type: ApplicationFiled: March 17, 2015Publication date: December 17, 2015Inventors: Robert E. Betzig, Kai Wang
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Publication number: 20140284461Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction.Type: ApplicationFiled: March 17, 2014Publication date: September 25, 2014Applicant: HOWARD HUGHES MEDICAL INSTITUTEInventor: Robert E. Betzig
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Publication number: 20140285653Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction.Type: ApplicationFiled: March 17, 2014Publication date: September 25, 2014Applicant: Howard Hughes Medical InstituteInventor: Robert E. Betzig
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Publication number: 20140285651Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction.Type: ApplicationFiled: March 17, 2014Publication date: September 25, 2014Applicant: HOWARD HUGHES MEDICAL INSTITUTEInventor: Robert E. Betzig
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Publication number: 20140198200Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction.Type: ApplicationFiled: March 17, 2014Publication date: July 17, 2014Applicant: Howard Hughes Medical InstituteInventor: Robert E. Betzig
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Publication number: 20140198201Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction.Type: ApplicationFiled: March 17, 2014Publication date: July 17, 2014Applicant: HOWARD HUGHES MEDICAL INSTITUTEInventor: Robert E. Betzig
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Patent number: 8711211Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction. A detector is configured for detecting signal light received by the imaging optics, and an aperture mask is positioned.Type: GrantFiled: June 14, 2011Date of Patent: April 29, 2014Assignee: Howard Hughes Medical InstituteInventor: Robert E. Betzig