Abstract: A circuit for use with an oximeter system which provides constant current pulses to light-emitting diodes in a sequential and cyclical manner. The circuit maintains magnitudes of pulses in the pulse train equal to certain other pulses in the pulse train over extremes of temperature variation, voltage variation, and time duration. Each diode is energized by a series of pulses having the same magnitude, although pulse magnitudes will vary from diode to diode. Therefore, light output from one light-emitting diode remains proportional to light output from the others.

Abstract: An electrical circuit for operating upon a slowly varying input signal (on the order of one volt per minute) to provide an output signal representative of the input signal derivative. The circuitry employs at least two feedback loops, one of which is constructed similar to an integrator amplifier. One particularly useful electro-medical application of this apparatus and method lies in the measurement of osmotic fragility of red blood cells, where percentage hemolysis of the blood slowly varies as a function of tonicity of suspension of the medium in which the blood sample is suspended. In this measurement the exact point of maximum slope is important and is provided by the present invention.