Abstract: Provided herein are E. coli host strains with improved capacity for producing recombinant proteins.

Abstract: Provided herein are methods for the improved production of periplasmic-targeted recombinant proteins in E. coli host strains. Also provided are E. coli host strains with improved capacity for producing recombinant proteins.

Abstract: Provided herein are methods for the improved production of periplasmic-targeted recombinant proteins in E. coli host strains. Also provided are E. coli host strains with improved capacity for producing recombinant proteins.

Abstract: Rediced genome or native K12 strain E. coli bacteria comprising expression vectors encoding a recombinant CRM197 protein and their use in the production of CRM 197 is provided. The CRM 197 protein may be fused to a signal sequence that directs the expressed CRM197 protein to the periplasm of the E. coli host.

Abstract: E. coli bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.

Abstract: E. coli bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.

Abstract: Rediced genome or native K12 strain E. coli bacteria comprising expression vectors encoding a recombinant CRM197 protein and their use in the production of CRM 197 is provided. The CRM 197 protein may be fused to a signal sequence that directs the expressed CRM197 protein to the periplasm of the E. coli host.

Abstract: E. coli bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.

Abstract: A triple host vector is described which is capable of expressing an inserted protein coding DNA sequence in any of three distinct host systems. The vector includes three promoters, one each for vertebrate, bacterial and insect host cells, so that the protein coding sequence can be cloned into the vector once and the vector can be used to express protein in all three types of host cells. The arrangement and selection of the promoters contributes to expression characteristics in all three host systems that is comparable to single host expression vectors.

Abstract: A triple host vector is described which is capable of expressing an inserted protein coding DNA sequence in any of three distinct host systems. The vector includes three promoters, one each for vertebrate, bacterial and insect host cells, so that the protein coding sequence can be cloned into the vector once and the vector can be used to express protein in all three types of host cells. The arrangement and selection of the promoters contributes to expression characteristics in all three host systems that is comparable to single host expression vectors.

Abstract: An antibody raised against an epitope of a peptide having an amino acid sequence selected from a group consisting of Leu Asp Gly Tyr Pro Ser Asp Tyr Asp Pro Phe Val Glu Asn Ala Gln Ile Tyr (SEQ ID NO:1) and Leu Asp Gly Tyr Pro Ser Asp Tyr Asp Pro Phe Glu Glu Asn Ala Gln Ile Tyr (SEQ ID NO:2) is advantageously used in an indirect immunoassay to detect the presence of active Human Herpesvirus-6 in a patient sample.

Abstract: Recombinant rabbit tissue factor is cloned and produced in a bacterial host. This protein, which is relatively insoluble and has several disulfide bonds, requires special modifications in order to express and be purified at commercial levels. By expressing the tissue factor as a fusion protein with a bacterial enzyme, thioredoxin, solubility is increased. Use of a thioredoxin reductase deficient host aids in proper tertiary structure for biological activity.

Abstract: A vector for the direct cloning of the products of PCR protocol incorporates single nucleotide overhangs at one or both ends of a linearized DNA segment. The single nucleotide overhangs are uracil or inosine residues, as desired, to facilitate cloning of the desired PCR products.

Abstract: An antibody raised against an epitope of a peptide having an amino acid sequence selected from a group consisting of LDGYPSDYDPFVENAQIY (SEQ ID NO:1) and LDGYPSDYDPFEENAQIY (SEQ ID NO:2) is advantageously used in an indirect immunoassay to detect the presence of active Human Herpesvirus-6 in a patient sample.