Abstract: A cryogenic cooling manifold and methods incorporating a cryogenic cooling manifold for managing the gas layer(s) above a liquid cryogen to control cooling temperature-time profiles and ice formation for microliter and smaller samples that are plunged through the gas and into the liquid cryogen.

Abstract: Sample support designs and sample cooling devices may be sued for single-particle cryo-electron microscopy. At least some of these sample support design and sample cooling devices help to simplify sample preparation and handling, to dramatically reduce errors and improve outcome reproducibility, and to dramatically reduce overall costs. A cryo-EM system includes, singly and in combination, a grid-based sample support system, grid handling tools, grid blotting tools, a plunge cooling system, and jet cooling systems.

Abstract: A fixed target sample holder for serial synchrotron crystallography comprising goniometer compatible base, a carrier, a sample holding insert which can be placed into the carrier. The sample holing insert comprising fiducials and windows, wherein each of the windows are respectively configured to accept a sample. The windows can also have holes and texture within each window. Additionally, a sample loading workstation for loading crystals into the sample holder and the removal of excess liquid from the sample, comprising a humidity-controlled chamber, a sample support within the chamber, a capture to place the goniometer-compatible base and a channel in communication with the chamber that allows for the flow of humidified air into the chamber.

Abstract: Presented are systems, methods, and devices for provisioning controlled sample support, precision sample immersion, and/or post-cooling sample storage of cryo-electron microscopy (cryo-EM) samples and grids. A puck is presented for manual or automated storage of cryo-EM sample grids after the grids and samples have been cryocooled. The puck carries one or more cryo-EM sample grids and includes a puck top portion and a puck bottom portion, each formed with a cryogenic-temperature compatible material. The bottom portion includes multiple receptacles that each accepts and holds a sample grid. The top portion includes an array of funnels. The puck top and bottom portions are assembled together by inserting the top portion into the bottom portion. When the puck may be immersed in a liquid cryogen, each funnel guides a sample grid released into an upper portion of the funnel downward and into a grid receptacle within a bottom portion of the funnel.

Abstract: The invention is directed to a sample support design and sample cooling devices for single-particle cryo-electron microscopy that simplify sample preparation and handling, dramatically reduce errors and improve outcome reproducibility, and dramatically reduce overall costs. A system includes a grid based sample support system, grid handling tools, grid blotting tools, a plunge cooling system, and jet cooling systems.

Abstract: Humidified sample preparation station for serial crystallography according to one embodiment is a humidified enclosure that delivers relative humidities above 95% and preferably above 97% in standard operation, and that can allow microscope observation of samples within. Humidified sample preparation station for serial crystallography can be used for preparation of protein crystal samples for examination using X-rays and for protein structure determination by X-ray crystallography, involving addition of liquid to the sample and removal of liquid from the sample using vacuum or suction.

Abstract: A fixed target sample holder for serial synchrotron crystallography comprising goniometer compatible base, a carrier, a sample holding insert which can be placed into the carrier. The sample holing insert comprising fiducials and windows, wherein each of the windows are respectively configured to accept a sample. The windows can also have holes and texture within each window. Additionally, a sample loading workstation for loading crystals into the sample holder and the removal of excess liquid from the sample, comprising a humidity-controlled chamber, a sample support within the chamber, a capture to place the goniometer-compatible base and a channel in communication with the chamber that allows for the flow of humidified air into the chamber.

Abstract: Presented are systems, methods, and devices for provisioning controlled sample support, precision sample immersion, and/or post-cooling sample storage of cryo-electron microscopy (cryo-EM) samples and grids. A puck is presented for manual or automated storage of cryo-EM sample grids after the grids and samples have been cryocooled. The puck carries one or more cryo-EM sample grids and includes a puck top portion and a puck bottom portion, each formed with a cryogenic-temperature compatible material. The bottom portion includes multiple receptacles that each accepts and holds a sample grid. The top portion includes an array of funnels. The puck top and bottom portions are assembled together by inserting the top portion into the bottom portion. When the puck may be immersed in a liquid cryogen, each funnel guides a sample grid released into an upper portion of the funnel downward and into a grid receptacle within a bottom portion of the funnel.

Abstract: Presented are systems, methods, and devices for provisioning sample support, controlled liquid deposition, and/or desired sample thicknesses during cryo-electron microscopy processes. A sample support for a cryo-electron microscopy process includes a support grid with multiple grid faces. The grid may be substantially rigid, flat and circular, may have a diameter of between about 2.9 and 3.1 mm, and may have a thickness of about 10 to 25 ?m. A foil covers at least one of the grid faces and includes multiple foil regions that each has a distinct thickness. The foil may be a thin and flexible metal or carbon foil. At least one of the foil regions contains an array of through-holes. A boundary between the foil regions with distinct thicknesses forms a step edge that pins thereto a receding contact line of a liquid drop deposited on the foil.

Abstract: A fixed target sample holder for serial synchrotron crystallography comprising goniometer compatible base, a carrier, a sample holding insert which can be placed into the carrier. The sample holing insert comprising fiducials and windows, wherein each of the windows are respectively configured to accept a sample. The windows can also have holes and texture within each window. Additionally, a sample loading workstation for loading crystals into the sample holder and the removal of excess liquid from the sample, comprising a humidity-controlled chamber, a sample support within the chamber, a capture to place the goniometer-compatible base and a channel in communication with the chamber that allows for the flow of humidified air into the chamber.

Abstract: A fixed target sample holder for serial synchrotron crystallography comprising goniometer compatible base, a carrier, a sample holding insert which can be placed into the carrier. The sample holing insert comprising fiducials and windows, wherein each of the windows are respectively configured to accept a sample. The windows can also have holes and texture within each window. Additionally, a sample loading workstation for loading crystals into the sample holder and the removal of excess liquid from the sample, comprising a humidity-controlled chamber, a sample support within the chamber, a capture to place the goniometer-compatible base and a channel in communication with the chamber that allows for the flow of humidified air into the chamber.

Abstract: Humidified sample preparation station for serial crystallography according to one embodiment is a humidified enclosure that delivers relative humidities above 95% and preferably above 97% in standard operation, and that can allow microscope observation of samples within. Humidified sample preparation station for serial crystallography can be used for preparation of protein crystal samples for examination using X-rays and for protein structure determination by X-ray crystallography, involving addition of liquid to the sample and removal of liquid from the sample using vacuum or suction.

Abstract: A fixed target sample holder for serial synchrotron crystallography comprising a goniometer compatible base, a carrier, a sample holding insert which can be placed into the carrier. The sample holding insert comprising fiducials and windows, wherein each of the windows are respectively configured to accept a sample. The windows can also have holes and texture within each window. Additionally, a sample loading workstation for loading crystals into the sample holder and the removal of excess liquid from the sample, comprising a humidity-controlled chamber, a sample support within the chamber, a capture to place the goniometer-compatible base, and a channel in communication with the chamber that allows for the flow of humidified air into the chamber.

Abstract: Humidified sample preparation station for serial crystallography according to one embodiment is a humidified enclosure that delivers relative humidities above 95% and preferably above 97% in standard operation, and that can allow microscope observation of samples within. Humidified sample preparation station for serial crystallography can be used for preparation of protein crystal samples for examination using X-rays and for protein structure determination by X-ray crystallography, involving addition of liquid to the sample and removal of liquid from the sample using vacuum or suction.

Abstract: A cryogenic cooling manifold and methods incorporating a cryogenic cooling manifold for managing the gas layer(s) above a liquid cryogen to control cooling temperature-time profiles and ice formation for microliter and smaller samples that are plunged through the gas and into the liquid cryogen.

Abstract: A cryogenic cooling manifold and methods incorporating a cryogenic cooling manifold for managing the gas layer(s) above a liquid cryogen to control cooling temperature-time profiles and ice formation for microliter and smaller samples that are plunged through the gas and into the liquid cryogen.

Abstract: Sample support design and sample cooling devices for single-particle cryo-electron microscopy that simplify sample preparation and handling, dramatically reduce errors and improve outcome reproducibility, and dramatically reduce overall costs. The system consisting of grid based sample support system, grid handling tools, grid blotting tools, a plunge cooling system, and jet cooling systems.

Abstract: A fixed target sample holder for serial synchrotron crystallography comprising a goniometer compatible base, a carrier, a sample holding insert which can be placed into the carrier. The sample holding insert comprising fiducials and windows, wherein each of the windows are respectively configured to accept a sample. The windows can also have holes and texture within each window. Additionally, a sample loading workstation for loading crystals into the sample holder and the removal of excess liquid from the sample, comprising a humidity-controlled chamber, a sample support within the chamber, a capture to place the goniometer-compatible base, and a channel in communication with the chamber that allows for the flow of humidified air into the chamber.

Abstract: Humidified sample preparation station for serial crystallography according to one embodiment is a humidified enclosure that delivers relative humidities above 95% and preferably above 97% in standard operation, and that can allow microscope observation of samples within. Humidified sample preparation station for serial crystallography can be used for preparation of protein crystal samples for examination using X-rays and for protein structure determination by X-ray crystallography, involving addition of liquid to the sample and removal of liquid from the sample using vacuum or suction.

Abstract: Apparatus and methods for performing small angle X-ray scattering (SAXS) at low (cryogenic) temperatures for determining the structure of and changes in the structure of proteins, DNA, RNA, and other biological molecules and biomolecular assemblies and structures. A cryogenic, small angle X-ray scattering (SAXS) application sample holder, includes a sample cell including a base portion and at least two parallel walls disposed on the base, wherein the sample cell has a liquid volume capacity defined by the walls and the base portion of 0.001 to 10 microliters.