Patents by Inventor Robert F. Ramig

Robert F. Ramig has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • In vitro replication system capable of rescuing cloned and manipulated rotavirus genes
    Patent number: 5614403
    Abstract: A method for template-dependent in vitro replication and transcapsidation of double stranded RNA from Reoviridae mRNA templates is described. This method provides an efficient means for isolating rotaviral mRNA, manipulating the mRNA, and expressing the new dsRNA species without the use of infected cells by constructing a new virus in the test tube without any cellular components. Viral protein complexes are prepared by test tube degradation of purified virus particles or by expression of a small subset of viral genes in insect cells, where the proteins form complexes. Viral mRNAs are made in the test tube by transcription of "native" viral mRNAs from viral transcriptase particles previously made in vitro, or by expression of viral mRNAs from transcription vectors in vitro.
    Type: Grant
    Filed: June 1, 1994
    Date of Patent: March 25, 1997
    Assignee: Baylor College of Medicine
    Inventors: Robert F. Ramig, Dayue Chen