Abstract: A proess, and a composition of matter utilized therein, to obtain transformation of the glochidia of freshwater mussels to the juvenile stage. In nature, glochidia normally transform while as parasites on fish. The instant artificial process involves the use of cell culture and bacteriological techniques to best assure environmental integrity during the protracted culture period. The composition of the media includes a combination of the blood plasma of fish and commonly available tissue culture fluids and inorganic salts. A nonspecific component of fish blood plasma in combination with the media, supra, was determined to be essential for glochidial transformation to juveniles.

Abstract: A process, and a composition of matter utilized therein, to obtain transformation of the glochidia of freshwater mussels to the juvenile stage. In nature, glochidia normally transform while as parasites on fish. The instant artificial process involves the use of cell culture and bacteriological techniques to best assure environmental integrity during the protracted culture period. The composition of the media includes a combination of the blood serum of fish and commonly available tissue culture fluids and inorganic salts. A nonspecific component of fish serum in combination with the media supra was determined to be essential for glochidial transformation to juveniles.