Abstract: Methods, reagents, kits and systems for characterizing different proteins of interest are provided. The provided methods, systems, etc. provide detection, characterization of proteins for different biologically relevant proteins for monitoring and characterizing biological processes.

Abstract: Methods, reagents, kits and systems for characterizing different proteins of interest are provided. The provided methods, systems, etc. provide detection, characterization of proteins for different biologically relevant proteins for monitoring and characterizing biological processes.

Abstract: Methods and systems are provided for detecting a binding interaction between a binding reagent and an analyte under equilibrium binding conditions. The binding interaction can be detected by the formation of an interaction identification moiety. The interaction identification moiety can be detected even after the binding reagent dissociates from the analyte.

Abstract: Methods for characterizing one or more molecules based upon detection of time-dependent changes in binding interactions between the molecules and binding entities are provided. Characterizations of individual molecules by provided methods include identification of the molecules and determination of previously uncharacterized time-dependent binding interactions between the molecules and binding entities.

Abstract: The present disclosure provides methods of determining association rates or dissociation rates between affinity reagents and proteins. The methods can be configured to monitor a large number of proteins in parallel, for example, using arrays of proteins that are contacted with solutions containing affinity reagents. The methods can be further configured to detect the arrayed proteins at single-molecule resolution. Accordingly the methods allow a large population of proteins to be monitored on an individual basis. As such binding kinetics and thermodynamics can be determined on a population level while allowing individual interactions to be evaluated.

Abstract: Provided are methods of detecting analytes. In some configurations, the methods can employ analytes attached to a solid support or particle and affinity reagents that are attached to the solid support or particle via a flexible linker. In some configurations, the methods can employ analytes attached to a solid support or particle and solution-phase affinity reagents can be attracted to the analytes via application of a stimulus.

Abstract: The present disclosure provides, inter alia, methods, compositions, and computer implemented processes for resolving long and highly similar, but non-identical, genomic regions to improve assembly quality, especially for polyploid genomes. Aspects of the disclosure are draw to using exact string matching of homopolymer-collapsed sequence reads to determine whether two sequences overlap and thus represent the same genomic region (e.g., the same haplotype in polyploid genomes) or whether the sequences represent different genomic regions.

Abstract: A method of tracking tagged transaction data corresponding to transactions between users and merchants is provided. The method includes receiving, by the tag tracking computing device, a plurality of transaction signals including a plurality of transaction data associated with the transactions. The method further includes receiving, by the tag tracking computing device, tagging data from a party, the tagging data including a tag and an identification of transaction data associated with a transaction. And, the method further includes appending, by the tag tracking computing device, the tag to transaction data stored in a database based on the tagging data from the party.

Abstract: A computational method system, and computer program are provided for inferring functional links from genome sequences. One method is based on the observation that some pairs of proteins A′ and B′ have homologs in another organism fused into a single protein chain AB. A trans-genome comparison of sequences can reveal these AB sequences, which are Rosetta Stone sequences because they decipher an interaction between A′ and B. Another method compares the genomic sequence of two or more organisms to create a phylogenetic profile for each protein indicating its presence or absence across all the genomes. The profile provides information regarding functional links between different families of proteins. In yet another method a combination of the above two methods is used to predict functional links.