Abstract: The present disclosure relates generally to methods and systems useful in imaging applications, especially biological imaging applications, and applications in the metrology, atmospheric, scientific and medical fields. In one aspect, the disclosure provides a method of imaging an object, including illuminating the object with incident radiation through one or more adaptive optical elements; receiving transmitted radiation from the object at a photodetector to provide a base image; and performing the following steps one or more times: adjusting the one or more adaptive optical elements, the adjustment including modifying an optical transfer function of the one or more adaptive optical elements, and receiving transmitted radiation from the object at the photodetector to provide an adjusted image; wherein the adjustment and receiving steps are performed until the adjusted image has substantially reduced aberrations compared to the base image.

Abstract: A wet seal standpipe cap to eliminate standpipe cap leakage includes a generally circular cap which has affixed to the underside of the cap a plurality of baffle rings. The cap is sized to enclose an opening in the top of a standpipe, and permit one of the baffle rings to penetrate a liquor contained in a canal placed around the standpipe opening providing a wet seal. The cap, in cooperation with the canal, entraps any leakage from around the standpipe opening between one of the baffle rings, underside of the cap and contained liquor.