Abstract: The invention relates to a cucumber (Cucumis sativus) plant, which has the improved shelf life as found in plants and fruits grown from seeds of cucumber EX5001 representative seeds of which were deposited under NCIMB accession number 41670. The plant may be obtainable by crossing a cucumber plant with a plant grown from seeds of cucumber EX5001 representative seeds of which were deposited under NCIMB accession number 41670 and selecting in the F2 progeny of the cross that may be obtained after selfing the F1 for plants showing an improved shelf life.

Abstract: Provided is a method for removing a liquid, in particular a supernatant, from a container with a sample, by creating an opening in a container with a sample consisting of solid constituents and a liquid, and subsequently centrifuging the container so as to cause the liquid to flow out of the container through the created opening. Also provided is a device for making a container suitable for use in the method.

Abstract: The present invention relates to a for obtaining a plant showing a reduced susceptibility towards infection with a pathogen, in particular an oomycete, comprising treating MO seeds of a plant species to be modified with a mutagenic agent to obtain M1 seeds and growing plants therefrom the thus obtained M1 seeds to obtain M1 plants, inoculation of the thus obtained M1+n plants with the pathogen and selecting plants that show a reduction or absence of sporulation of the pathogen as plants having a reduced susceptibility phenotype. The invention further relates to plants, seeds, pollen, cells and tissues that have the reduced susceptibility towards oomycetes.

Abstract: Provided is a method for mapping traits in organisms, in particular in plants. The method comprises a) providing a population of SDR-0 organisms, in particular plants, that each arise from one member of a population of unreduced cells resulting from second division restitution, in particular a population of unreduced spores; b) producing SDR-1 progeny populations of each of these SDR-0 organisms; c) phenotyping the SDR-1 progeny populations to identify segregating traits within each SDR-1 progeny population; d) if segregating progeny are present in a SDR-1 progeny population, genotyping the corresponding SDR-0 organism and comparing the genotype thereof with the genotype of the other SDR-0 organisms to identify heterozygous chromosomal regions associated with the occurrence of the segregating trait identified in the SDR-1 progeny population.

Abstract: Provided is a method for mapping traits in organisms, in particular in plants. The method comprises a) providing a population of SDR-0 organisms, in particular plants, that each arise from one member of a population of unreduced cells resulting from second division restitution, in particular a population of unreduced spores; b) producing SDR-1 progeny populations of each of these SDR-0 organisms; c) phenotyping the SDR-1 progeny populations to identify segregating traits within each SDR-1 progeny population; d) if segregating progeny are present in a SDR-1 progeny population, genotyping the corresponding SDR-0 organism and comparing the genotype thereof with the genotype of the other SDR-0 organisms to identify heterozygous chromosomal regions associated with the occurrence of the segregating trait identified in the SDR-1 progeny population.

Abstract: Provided is a method for screening a population of plants for the presence therein of individuals that show a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting and Yellowing, as compared to a control plant, wherein a population of seeds is germinated in darkness and in the presence of ethylene to obtain seedlings that, when having a longer hypocotyl as compared to the original ethylene-sensitive control under ethylene, are selected as plants showing a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting or Yellowing. Also provided are plants thus selected.

Abstract: Provided is a method for screening a population of plants for the presence therein of individuals that show a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting and Yellowing, as compared to a control plant, wherein a population of seeds is germinated in darkness and in the presence of ethylene to obtain seedlings that, when having a longer hypocotyl as compared to the original ethylene-sensitive control under ethylene, are selected as plants showing a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting or Yellowing. Also provided are plants thus selected.

Abstract: Provided is a method for removing a liquid, in particular a supernatant, from a container with a sample, by creating an opening in a container with a sample consisting of solid constituents and a liquid, and subsequently centrifuging the container so as to cause the liquid to flow out of the container through the created opening. Also provided is a device for making a container suitable for use in the method.

Abstract: The present invention relates to a for obtaining a plant showing a reduced susceptibility towards infection with a pathogen, in particular an oomycete, comprising treating MO seeds of a plant species to be modified with a mutagenic agent to obtain M1 seeds and growing plants therefrom the thus obtained M1 seeds to obtain M1 plants, inoculation of the thus obtained M1+n plants with the pathogen and selecting plants that show a reduction or absence of sporulation of the pathogen as plants having a reduced susceptibility phenotype. The invention further relates to plants, seeds, pollen, cells and tissues that have the reduced susceptibility towards oomycetes.

Abstract: The invention relates to a method for producing haploid plant embryos, comprising providing microspores or pollen that comprise cell division inducing molecules; pollinating an embryo sac cell, in particular an egg cell, of the plant of which the haploid embryo is to be made with the microspores or pollen; allowing the microspores or pollen to discharge the cell division inducing molecules in or in the vicinity of the embryo sac cell, in particular the egg cell, to trigger division thereof to obtain a haploid plant embryo. When doubled haploid plant embryos are to be produced doubling of the chromosome number takes place at a certain stage after pollination, in particular during cell division or after obtaining the embryo. The invention further relates to the embryos thus obtained, plants regenerated therefrom and progeny thereof.