Abstract: Provided herein are mass spectrometric standards for distinguishing hemoglobin beta (HBB) and hemoglobin beta sickle (HBS) and methods of using these standards to determine the amount of HBB relative to HBS in a sample.

Abstract: Provided herein and methods and kits for identifying inhibitors of SUMOylation. The provided methods include contacting a candidate agent with (i) a SUMO-conjugating peptide comprising an N-terminal linker and a detectable tag, and (ii) a SUMO comprising a metal complex, under conditions that allow binding between the SUMO-conjugating peptide and the SUMO, and detecting the detectable tag thereby determining the level of binding between the SUMO-conjugating peptide and SUMO. A reduced level of binding as compared to the level of binding in the absence of the candidate agent indicating the agent inhibits SUMOylation.

Abstract: Provided herein and methods and kits for identifying inhibitors of SUMOylation. The provided methods include contacting a candidate agent with (i) a SUMO-conjugating peptide comprising an N-terminal linker and a detectable tag, and (ii) a SUMO comprising a metal complex, under conditions that allow binding between the SUMO-conjugating peptide and the SUMO, and detecting the detectable tag thereby determining the level of binding between the SUMO-conjugating peptide and SUMO. A reduced level of binding as compared to the level of binding in the absence of the candidate agent indicating the agent inhibits SUMOylation.

Abstract: A mineral composition comprised of at least 90 weight percent of roofing granules, wherein said roofing granules comprise at least 40 weight percent of calcium carbonate, have a hardgrove grindability index of less than 70, contain less than 1.0 percent of particles smaller than 250 microns, contain less than 1.0 percent of particles greater than 2380 microns, contain from about 2 to about 10 percent of particles greater than 1,680 microns, contain less than 2 percent of particles less than 40 microns, and have a Hunter L value of from about 20 to about 30.

Abstract: A roofing shingle that contains a roofing granules. The roofing granules contain at least about 95 weight percent of calcium carbonate with a hardgrove grindability index of less than about 70, and from about 0.1 to about 1.0 weight percent of a pigmented material; the pigmented material contains from about 10 to about 35 weight percent of pigment and from about 90 to about 65 weight percent of a resin; and the roofing granules are coated with an amine antistrip agent.

Abstract: A shingle assembly that contains a fiber mat and an asphalt mixture disposed within the fiber mat. The asphalt mixture contains asphalt and a mineral filler; a layer of mineral granules is disposed on the top surface of the shingle assembly; the mineral granules contain at least about 40 weight percent of limestone particles that have a hardgrove grindability index of less than 70; and the particles are coated with an oil.

Abstract: A process for making a composition of asphalt and filler in which a viscosity reducing additive is sprayed onto particles of shingle filler material to produce coated shingle filler material, the coated shingle filler material is heated, asphalt is heated, and the heated coated shingle filler material is mixed with the heated asphalt.

Abstract: A composition comprised of asphalt and filler, wherein said filler is comprised of particles that comprise an inorganic core and a coating disposed on said core, and wherein at least about 60 weight percent of said particles are smaller than about 212 microns.

Abstract: A roofing shingle that contains a roofing granules. The roofing granules contain at least about 95 weight percent of calcium carbonate with a hardgrove grindability index of less than about 70, and from about 0.1 to about 1.0 weight percent of a pigmented material; the pigmented material contains from about 10 to about 35 weight percent of pigment and from about 90 to about 65 weight percent of a resin; and the roofing granules are coated with an amine antistrip agent.

Abstract: A mineral composition comprised of at least about 90 weight percent of roofing granules, at least about 50 weight percent of calcium carbonate with a hardgrove grindability index of less than about 70, from about 0.1 to about 1.0 weight percent of a pigmented material, from about 0.1 to about 1.0 weight percent of a composition for inhibiting the growth of an organism selected from the group consisting of algae, bacteria, and mixtures thereof, and less than about 100 parts per million of a metal selected from the group consisting from the group consisting of arsenic, barium, cadmium, chromium, lead, mercury, selenium, and silver, and less than about 100 parts per million of a polycyclic aromatic hydrocarbon. The mineral composition, when tested in accordance with by ASTM Standard Test D 4977-03, loses less than 5 grams of material.

Abstract: A mineral composition comprised of at least about 90 weight percent of roofing granules, at least about 50 weight percent of calcium carbonate with a hardgrove grindability index of less than about 70, from about 0.1 to about 1.0 weight percent of a pigmented material, from about 0.1 to about 1.0 weight percent of a composition for inhibiting the growth of an organism selected from the group consisting of algae, bacteria, and mixtures thereof, and less than about 100 parts per million of a metal selected from the group consisting from the group consisting of arsenic, barium, cadmium, chromium, lead, mercury, selenium, and silver, and less than about 100 parts per million of a polycyclic aromatic hydrocarbon. The mineral composition, when tested in accordance with by ASTM Standard Test D 4977-03, loses less than 5 grams of material.

Abstract: Aircraft wing fatigue life and weight are strongly influenced by maximum wing root bending moment. The object of the invention is to augment the wing root bending moment relief that can be provided by the deflection of aircraft ailerons (12) in the conventional manner, while reducing or eliminating aileron induced wing torsional loading. To accomplish this, a movable aerodynamic control surface (14) is mounted on the outboard side of a contoured boom (13) situated at each wingtip. The exact size and location of the control surfaces (14) are determined by the particular aircraft application. Control surface size is a function of the aircraft aileron size, and each control surface (14) is located ahead of the elastic axis (10') of the wing (10). In addition to providing load alleviation, asymmetric deflection of the wingtip control surfaces (14) creates an aircraft rolling moment that adds to that produced by aileron deflection.