Abstract: The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence difference.

Abstract: Synthetic triple-helical peptides (THPs) are used for the design and synthesis of triple-helical transition state analog inhibitors. These inhibitors feature a phosphonate ester or phosphinic moiety in place of the scissle bond. These groups inhibit MMPs, and methods been developed for their convenient incorporation within a peptide sequence by solid-phase methods.

Abstract: The present invention attaches to the bottom of the used filter in a one-way locked fashion. It can be described as a concave surface, equipped with a plurality of one-way locking barbs, or locking means, that upon a quick insertion, or turning process into the normally provided center threaded channel of a spin-on oil filter, makes positive seal against the normally provided gasket in oil filters, transforming the filter into a sealed container or cylinder. The present invention is also equipped with a time-release oil-eating bacteria, or other suitable biological agent, to eventually render the filter residual oil inert to the environment if the filter is still illegally dumped. Therefore, the device prevents the oil filter from leaking shortly after it is removed and substantially hot drained, to facilitate transportation to a suitable recycling or collection center, thereby enabling the recovery and recycling of used oil filters.

Abstract: A transgenic rat expresses a gene of interest, such as EGFP, exclusively in the germ cells of both males and females. From such a transgenic rat one can isolate a line of spermatogonial stem cells, which can renew and proliferate in culture.

Abstract: The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

Abstract: The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

Abstract: The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

Abstract: The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

Abstract: The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

Abstract: The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

Abstract: The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

Abstract: Short peptides containing C?,?-dipropylglycine (Dpg) at alternating sequence positions were synthesized and examined for conformational behavior. Peptide assembly was performed using Fmoc-solid-phase chemistry where the coupling with PyAOP could be significantly enhanced at elevated temperature. Circular dichroism (CD) and NMR conformational studies revealed that incorporation of Dpg residues induced folded structures into peptides. It was observed that Dpg residues adopted helical conformation in a helix-promoting sequence. The resulting helical structure was comprised of consecutive ?-turns whose structure was stabilized by salt bridge in aqueous solution. In this study, the preparation of sterically and polyfunctional C?,?-disubstituted amino acids (??AAs) via alkylation of ethyl nitroacetate and transformation into derivatives ready for incorporation into peptides are described.

Abstract: A sensor designed for measurement of conducted heat flux passing through a solid object consists of a thin film thermopile deposited on a planar substrate whose thermal properties match those of the solid object. The thermopile is protected by a thin rectangular plate made of the same material as the substrate. The sensor is imbedded in the solid object and measures the vector of heat flux along the thermopile axis with minimal distortion of the heat flow pattern. Applications include measurement of heat flux in casting molds, boiler tubes, well surveying instruments and laser weapons testing.

Abstract: A sensor designed for measurement of conducted heat flux passing through a solid object consists of a thin film thermopile deposited on a planar substrate whose thermal properties match those of the solid object. The thermopile is protected by a thin rectangular plate made of the same material as the substrate. The sensor is imbedded in the solid object and measures the vector of heat flux along the thermopile axis with minimal distortion of the heat flow pattern. Applications include measurement of heat flux in casting molds, boiler tubes, well surveying instruments and laser weapons testing.

Abstract: A portable structure to be used by an individual in an upright or seated position. Such a structure will be assembled where desired by first assembling tube connectors and inserting the tube assemblies into the cup shaped receptors around the opposing major body components. This skeleton assembly will then be covered with a suitable flexible material using temporary "S" shaped hooks.

Abstract: A combined linear-rotary direct drive step motor, having a rotary section and a linear section in a single housing, comprising a cylindrically shaped variable reluctance linear step motor; a modified hybrid permanent-magnet rotary step motor; and a common shaft shared by said linear step motor and said rotary step motor. The teeth of the rotary motor are circumferentially spaced around the stator pole faces and rotor; while in the linear portion of the motor, the teeth are longitudinally spaced along the face of the stator poles and rotor. Except for the teeth, the shape of the stator poles in the rotary and linear sections are identical. The laminated stators and rotors reduce eddy current losses at high stepping speeds. The stator coils of both motor sections are separate and can be energized independently allowing any combination of rotary or linear motion. According to alternative embodiments of the motor, the rotor is fabricated from stacked laminations, in a waffle board like configuration.

Abstract: A combined linear-rotary direct drive step motor, having a rotary section and a linear section in a single housing, comprising a cylindrically shaped variable reluctance linear step motor; a modified hybrid permanent-magnet rotary step motor; and a common shaft shared by said linear step motor and said rotary step motor. The teeth of the rotary motor are circumferentially spaced around the stator pole faces and rotor; while in the linear portion of the motor, the teeth are longitudinally spaced along the face of the stator poles and rotor. Except for the teeth, the shape of the stator poles in the rotary and linear sections are identical. The laminated stators and rotors reduce eddy current losses at high stepping speeds. The stator coils of both motor sections are separate and can be energized independently allowing any combination of rotary or linear motion. According to alternate embodiments of the invention, the rotor is fabricated from stacked laminations, in a waffle board like configuration.

Abstract: An apparatus for the linear translation of read/write heads in a data recording system is disclosed herein. The apparatus comprises a compound parallel bending element suspension with a driver connected thereto. The compound parallel suspension comprises two sets of cantilevered bendable elements. One end of each bendable element is attached to a movable connector to connect the elements together. The other ends of two bendable elements are attached to a non-movable support post. The other ends of the remaining two elements are connected to the read/write head assembly and driver. The driver can be any of a number of conventional stepping and voice coil motor configurations including but not limited to friction drives, taut band drives, rack and pinion drives.

Abstract: Uniform holes with a diameter of the order of 1 micrometer or larger can be formed by an etching operation which employs the combination properties of the surface tension of the etch coupled with a force on the meniscus of the etch in the hole to cause the etching to stop when one hole reaches the proper size and to permit the etching to continue on all other holes until they reach the same size.

Abstract: The invention provides a reinforced mask which enables closely spaced strips or other narrowly separated parts of a deposited pattern to be formed upon a substrate by a non-line-of-sight deposition process (such as ion plating or sputter deposition) without causing unwanted voids or discontinuities to appear in the deposited pattern at points that underlie the reinforcing ribs on the mask. The mask is formed by a selective material removing process that produces a thin web adjoining one or more relatively thick ribs that provide rigidity to the web.