Abstract: Provided herein are methods for generating multiplex CRISPR arrays based on annealing and ligating single-stranded DNA oligonucleotides using bridge oligonucleotides. The methods described herein include providing a first oligonucleotide comprising a CRISPR repeat sequence or a portion thereof, and a first portion of a first spacer sequence at its 3? end; providing a second oligonucleotide comprising, from 5? to 3?, a second portion of the first spacer sequence, the CRISPR repeat sequence, and a first portion of a second spacer sequence; providing a bridge oligonucleotide comprising a sequence substantially complementary to the first spacer sequence; allowing the first oligonucleotide and the second oligonucleotide to hybridize with the bridge oligonucleotide; and ligating the first and second oligonucleotide.

Abstract: A central vacuum connectable to an interior portion of an inhabitable structure. The central vacuum system includes a housing having a first housing member and a second housing member secured to the first housing member. Together the first housing member and the second housing member at least partially define a collection chamber. A vacuum motor is supported by the housing and is operable to move debris from the interior portion into the collection chamber. An adapter extends into the collection chamber for supporting a bag. At least one of the bag and the adapter are accessible through an opening defined in one of the first housing member and the second housing member.

Abstract: A central vacuum system connectable to an interior portion of an inhabitable structure includes a housing having an upper end, a lower end, and a side wall defining a collection chamber, the side wall defining an opening communicating between atmosphere and the collection chamber, and a vacuum motor supported in the housing and being operable to move debris from the interior portion into the collection chamber.

Abstract: The invention is directed to an intercom system for communication between persons on opposite sides of an entry door or the like. On a first embodiment the intercom device is energized by the person on the exterior of the door when a combined power and door buzzer or bell switch is depressed. When energized, voice communication can be established by the combined speaker, microphone and amplifying devices. A sound activated switch positioned on the interior side of the entry door allows hands free communication between the persons on the opposite sides of the door. A timer terminates the system power after a predetermined time span. In a second embodiment, a pair of radio frequency transceivers are employed with the intercom device to allow communication by the person on the interior side of the door who is located remote from the door.