Abstract: Splice site-specific primers for increasing the informational content of AFLP fingerprints, a method for splice site specific fingerprinting, and a method for targeting genic regions based on conserved splice sites sequences. The invention further describes the conversion into a PCR assay of the splice site-specific fragments obtained by the method of the invention.

Abstract: A method for efficiently producing homozygous organisms from a heterozygous non-human starting organism, comprising providing of a heterozygous starting organism; allowing the starting organism to produce haploid cells; creating homozygous organisms from the haploid cells thus obtained; and selecting the organisms having the desired set of chromosomes, wherein during production of the haploid cells no recombination occurs in order to obtain a limited number of genetically different haploid cells. Recombination can also be prevented or suppressed.