Patents by Inventor Robert J. Duronio

Robert J. Duronio has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for protein N-myristoylation
    Patent number: 5504008
    Abstract: There is disclosed a method for providing for the coexpression of N-myristoyltransferase and a protein substrate for said N-myristoyltransferase in E. coli comprising introducing into E. coli a dual plasmid system comprising (A) the isopropyl-.beta.-D-thiogalactopyranoside-inducible tac promoter, the g10-L ribosome binding site, a NMT gene, the kanamycin resistance gene and the p15A origin of replication in operable sequence and (B) the recA promoter, the g10-L ribosome binding site, a mammalian gene, the ampicillin resistance gene and the Col E1 origin of replication in operable sequence. This allows production of mammalian N-myristoylproteins or proteins containing covalently linked analogs of myristate with altered physical-chemical properties.
    Type: Grant
    Filed: July 13, 1994
    Date of Patent: April 2, 1996
    Assignee: Washington University
    Inventors: Robert J. Duronio, Peter O. Olins, Jeffrey I. Gordon
  • Method for protein N-myristoylation
    Patent number: 5436138
    Abstract: There is disclosed a method for providing for the coexpression of N-myristoyltransferase and a protein substrate for said N-myristoyltransferase in E. coli comprising introducing into E. coli a dual plasmid system comprising (A) the isopropyl-.beta.-D-thiogalactopyranoside-inducible tac promoter, the g10-L ribosome binding site, a NMT gene, the kanamycin resistance gene and the p15A origin of replication in operable sequence and (B) the recA promoter, the g10-L ribosome binding site, a mammalian gene, the ampicillin resistance gene and the Col E1 origin of replication in operable sequence. This allows production of mammalian N-myristoylproteins or proteins containing covalently linked analogs of myristate with altered physical-chemical properties.
    Type: Grant
    Filed: July 12, 1993
    Date of Patent: July 25, 1995
    Assignee: Washington University
    Inventors: Robert J. Duronio, Peter O. Olins, Jeffrey I. Gordon