Abstract: Provided herein are recombinant miniature swine expressing human CD47 in a tissue specific fashion. Also provided are kidneys isolated from a miniature swine, wherein the glomeruli of the kidney express human CD47 at a level higher than the level of human CD47 expression in the tubules of the kidney. Furthermore, provided herein are methods of transplanting such swine kidneys with glomeruli-specific expression of human CD47 from recombinant miniature swine into human recipients. In certain aspects, provided herein are methods of transplantation comprising transplanting hematopoietic stem cells expressing human CD47 from a first donor animal (e.g., a miniature swine) and a kidney expressing human CD47 in the glomeruli from a second donor animal (e g., a miniature swine) to a recipient (e.g., a human recipient).

Abstract: The present disclosure provides for transgenic swine, comprising one or more nucleotide sequences encoding one or more HLA I polypeptides and/or one or more HLA II polypeptides inserted into one or more native SLA loci of the swine genome, methods of making and methods of using. The present disclosure also provides for improved methods of making human immune system mice.

Abstract: Aspects of a detention pond retrofit device for detention basin outlet control provide structures designed to throttle stormwater flow and maintain the rate at which water is discharged from the system below Qcritical, the flow rate at which erosion and down cutting of the receiving stream would begin, based on channel morphology and bed material resistance. The primary goal of the device is to induce increased flow detention during low to moderate rain events, while providing similar hydraulic performance during large events to that of the detention basin performance prior to the installation of the retrofit unit. This is achieved through a “T” or “Y” design with split flow paths, in which the lower path is hydraulically restricted through the installation of structures while the upper path is unrestricted.

Abstract: Aspects of a detention pond retrofit device for detention basin outlet control provide structures designed to throttle stormwater flow and maintain the rate at which water is discharged from the system below Qcritical, the flow rate at which erosion and down cutting of the receiving stream would begin, based on channel morphology and bed material resistance. The primary goal of the device is to induce increased flow detention during low to moderate rain events, while providing similar hydraulic performance during large events to that of the detention basin performance prior to the installation of the retrofit unit. This is achieved through a “T” or “Y” design with split flow paths, in which the lower path is hydraulically restricted through the installation of structures while the upper path is unrestricted.

Abstract: The invention relates to the genetic manipulation of non-human animals. More particularly, the invention relates to genetic manipulation of non-human animals to be used for xenotransplantation. The invention provides a method of selecting GGTA 1 null cells, a viable GGTA 1 null swine, methods for making such swine, and methods of using cells, tissues and organs of such swine for xenotransplantation.

Abstract: The invention relates to the genetic manipulation of non-human animals. More particularly, the invention relates to genetic manipulation of non-human animals to be used for xenotransplantation. The invention provides viable gene knockout swine including swine in which the ?(1,3)-galactosyltransferase gene has been disrupted, methods for making such swine, and methods of using the tissues and organs of such swine for xenotransplantation.

Abstract: An adjustable, modular transport system for safely moving floral arrangements for funerals and other events by means of a truck or van. The system includes a base unit of various lengths having an elongated base with groove-forming rails mounted on the sides of the base along the length thereof to form respective inward facing grooves. The base floor and the inward facing grooves form a slot. The rails contained bores spaced therealong at equal intervals to accommodate spacers having corresponding rods extending downward therefrom. The slot accommodates a conventional floral basket base extension. A number of baskets may be inserted and separated by the spacers installed therebetween. The slot accommodate a base support for a flower pot or vase holder and of the same dimensions as that of the conventional basket base. The flower vase holder is tubular in shape, vertically mounted on an end.

Abstract: The invention provides a method and system for enforcing file access control among client devices using multiple diverse access control models and multiple diverse file server protocols. A multi-protocol file server identifies each file with one particular access control model out of a plurality of possible models, and enforces that one particular model for all accesses to that file. When the file server receives a file server request for that file using a different access control model, the file server translates the access control limits for that file into no-less-restrictive limits in the different model. The file server restricts access by the client device using the translated access control limits. Each file is assigned the access control model of the user who created the file or who last set access control limits for the file. When a user having a different access control model sets access control limits, the access control model for the file is changed to the new model.

Abstract: Porcine CD34, oligonucleotides encoding the porcine CD34, porcine CD34 antibodies and methods of making and using each are disclosed.

Abstract: A method of enhancing tolerance of a porcine transplant in a xenogeneic recipient by administering porcine bone marrow cells to the recipient and of enhancing proliferation and engraftment of the porcine bone marrow cells by exposing said cells to at least one substantially pure porcine cytokine and porcine cytokines that are substantially free of other porcine proteins and preferentially enhance the proliferation and engraftment of porcine bone marrow cells in the presence of bone marrow cells of other species. Protein and DNA sequence(s) for such porcine cytokines.

Abstract: A method of enhancing tolerance of a porcine transplant in a xenogeneic recipient by administering porcine bone marrow cells to the recipient and of enhancing proliferation and engraftment of the porcine bone marrow cells by exposing said cells to at least one substantially pure porcine cytokine and porcine cytokines that are substantially free of other porcine proteins and preferentially enhance the proliferation and engraftment of porcine bone marrow cells in the presence of bone marrow cells of other species. Protein and DNA sequence(s) for such porcine cytokines.

Abstract: A method of enhancing tolerance of a porcine transplant in a xenogeneic recipient by administering porcine bone marrow cells to the recipient and of enhancing proliferation and engraftment of the porcine bone marrow cells by exposing said cells to at least one substantially pure porcine cytokine and porcine cytokines that are substantially free of other porcine proteins and preferentially enhance the proliferation and engraftment of porcine bone marrow cells in the presence of bone marrow cells of other species. Protein and DNA sequence(s) for such porcine cytokines.

Abstract: A program debugging system has a core unit that includes a plurality of debugger memory areas, each uniquely associated with a corresponding one of a plurality of debuggers. The core unit responds to an exception condition by selecting one debugger from the plurality of debuggers, selection being made by determining which one of the debuggers is associated with the program exception. Then, computer state information and debugger state information are stored into a selected one of the debugger memory areas that is exclusively associated with the selected debugger, and the selected debugger is activated. A new debugger may register with the core unit, so that the new debugger is added to the plurality of debuggers. The activated debugger may send a debugging command to the core unit, which responds by updating debugger state information based on the received debugging command, and storing the updated debugger state information into the selected debugger memory area.