Abstract: Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.

Abstract: An electrical insulating and heating element support assembly for a high temperature vacuum furnace having a threaded support rod for connecting a heating element to the insulated hot-zone support ring in an electrically non-connected position includes insulator sleeves and washers surrounding the rod in contact with a series of refractory metal washers which may include graphite and/or molybdenum as shielding liners used to protect electrical insulators from having electrical short path means due to deposition of conductive materials onto the non-conducting insulators, and the use of threaded nuts and bushings to anchor the rod and shielding arrangement within the furnace hot zone. The non-conducting insulators and washers are made from materials with high thermal and electrical resistance, such as preferably alumina or mullite, and radially surround the support rod and the heating element.

Abstract: A vacuum furnace for heat treating and rapid gas quenching insitu a work piece at pressures up to 20 Bar and high gas velocities. The furnace comprises a single chamber having an inner portion including a plurality of graphite heating elements and graphite insulation, an outer portion and an access door. Further included are movable radiation baffle doors. In their closed position hot gases heat treat the work piece. In their open position the hot gases escape through the opening created onto chevron-shaped baffles in the outer portion which reflect radiation heat energy back into the inner portion and diffuse only convective heat energy into a heat exchanger and a variable speed drive recirculating fan. The recirculating fan directs the cooling gases through cooling nozzles onto the work piece to quench it to a pre-programmed temperature.

Abstract: The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.

Abstract: Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.

Abstract: The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound

Abstract: Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.

Abstract: The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound

Abstract: A machine is operable in a basic mode and a bonus mode. The bonus game is entered upon the occurrence of a start-bonus game outcome in the basic mode. In the bonus game, a player may select from an array of windows, each associated with a bonus game outcome. Credits are awarded based upon which ones of the windows are selected. The bonus game may end upon selection of a window associated with an end-bonus outcome but otherwise continues, allowing the player to make further selections until encountering an end-bonus outcome. A bonus game resource obtained in the basic game may be exercised in the bonus game to affect the bonus game outcome. For example, where the occurrence of an end-bonus outcome would otherwise end the bonus game, a player having a bonus game resource may exercise the bonus game resource to continue playing the bonus game.

Abstract: A slot machine operable is in a basic mode and a bonus mode. The bonus game is entered upon the occurrence of start-bonus game outcome in the basic mode. In the bonus game, a player may select from an array of windows, each associated with a bonus game outcome. Credits are awarded based upon which ones of the windows are selected. The bonus game may end upon selection of a window associated with an end-bonus outcome but otherwise continues, allowing the player to make further selections until encountering an end-bonus outcome. A bonus game resource obtained in the basic game may be exercised in the bonus game to affect the bonus game outcome. For example, where the occurrence of an end-bonus outcome would otherwise end the bonus game, a player having a bonus game resource may exercise the bonus game resource to continue playing the bonus game.

Abstract: A vacuum furnace for heat treating and rapid gas quenching insitu a work piece at pressures up to 20 Bar and high gas velocities. The furnace comprises a single chamber having an inner portion including a plurality of graphite heating elements and graphite insulation, an outer portion and an access door. Further included are movable radiation baffle doors. In their closed position hot gases heat treat the work piece. In their open position the hot gases escape through the opening created onto chevron-shaped baffles in the outer portion which reflect radiation heat energy back into the inner portion and diffuse only convective heat energy into a heat exchanger and a variable speed drive recirculating fan. The recirculating fan directs the cooling gases through cooling nozzles onto the work piece to quench it to a pre-programmed temperature.

Abstract: A Raman-active nanoparticle is provided that includes a dish-shape plasmonically active metal base, and a plasmonically active metal pillar disposed on the plasmonically active metal base, where the plasmonically active metal pillar is disposed within the dish-shape plasmonically active metal base and normal to a bottom of the dish-shape plasmonically active metal base, where a circular gap is disposed between the dish-shape plasmonically active metal pillar and inner walls of the dish-shape plasmonically active metal base. In one embodiment a Raman-active nanoparticle is provided that includes a dish-shape base having a dielectric material, an electrically conductive layer disposed on the inner surface of the dish-shape base, and an electrically conductive pillar disposed on the conductive layer, and within the dish-shape and perpendicular to a bottom of the dish-shape base, where a circular gap is disposed between the conductive pillar and inner walls of the dish-shape base.

Abstract: A slot machine operable is in a basic mode and a bonus mode. The bonus game is entered upon the occurrence of start-bonus game outcome in the basic mode. In the bonus game, a player may select from an array of windows, each associated with a bonus game outcome. Credits are awarded based upon which ones of the windows are selected. The bonus game may end upon selection of a window associated with an end-bonus outcome but otherwise continues, allowing the player to make further selections until encountering an end-bonus outcome. A bonus game resource obtained in the basic game may be exercised in the bonus game to affect the bonus game outcome. For example, where the occurrence of an end-bonus outcome would otherwise end the bonus game, a player having a bonus game resource may exercise the bonus game resource to continue playing the bonus game.

Abstract: A magnetic sifter is adapted for manipulation of biological cells by providing a greater pore density at the edge of the sifter than at the center. Application of an external magnetic field to the sifter causes high magnetic fields and field gradients at the sifter pores. These conditions are suitable for capturing magnetically tagged or labeled cells at the sifter pores. Altering the external magnetic field can provide controlled capture and/or release of magnetically labeled cells from the sifter pores. The purpose of having a greater pore density at the periphery of the sifter than at the center is to provide improved flow rate uniformity through the sifter. Such flow rate uniformity is advantageous for cell quantification.

Abstract: A bonus game for a slot machine operable in a basic mode and a bonus mode. The bonus game is entered upon the occurrence of a special start-bonus game outcome in the basic mode. In the bonus game, a player selects, one at a time, from an array of windows each associated with a bonus game outcome. Credits are awarded based upon which ones of the windows are selected. The bonus game ends upon selection of a window associated with an end-bonus outcome but otherwise continues, allowing the player to make further selections and accumulate further credits until encountering an end-bonus outcome. In one embodiment, a bonus game resource obtained in the basic game may be exercised in the bonus game to affect the bonus game outcome. In one embodiment, for example, where the occurrence of an end-bonus outcome would otherwise end the bonus game, a player having a bonus game resource may exercise the bonus game resource upon encountering an end-bonus outcome to continue playing the bonus game.

Abstract: A Raman-active nanoparticle is provided that includes a dish-shape plasmonically active metal base, and a plasmonically active metal pillar disposed on the plasmonically active metal base, where the plasmonically active metal pillar is disposed within the dish-shape plasmonically active metal base and normal to a bottom of the dish-shape plasmonically active metal base, where a circular gap is disposed between the dish-shape plasmonically active metal pillar and inner walls of the dish-shape plasmonically active metal base. In one embodiment a Raman-active nanoparticle is provided that includes a dish-shape base having a dielectric material, an electrically conductive layer disposed on the inner surface of the dish-shape base, and an electrically conductive pillar disposed on the conductive layer, and within the dish-shape and perpendicular to a bottom of the dish-shape base, where a circular gap is disposed between the conductive pillar and inner walls of the dish-shape base.

Abstract: A bonus game for a slot machine operable in a basic mode and a bonus mode. The bonus game is entered upon the occurrence of a special start-bonus game outcome in the basic mode. In the bonus game, a player selects, one at a time, from an array of windows each associated with a bonus game outcome. Credits are awarded based upon which ones of the windows are selected. The bonus game ends upon selection of a window associated with an end-bonus outcome but otherwise continues, allowing the player to make further selections and accumulate further credits until encountering an end-bonus outcome. In one embodiment, a bonus game resource obtained in the basic game may be exercised in the bonus game to affect the bonus game outcome. In one embodiment, for example, where the occurrence of an end-bonus outcome would otherwise end the bonus game, a player having a bonus game resource may exercise the bonus game resource upon encountering an end-bonus outcome to continue playing the bonus game.

Abstract: A magnetic sifter is adapted for manipulation of biological cells by providing a greater pore density at the edge of the sifter than at the center. Application of an external magnetic field to the sifter causes high magnetic fields and field gradients at the sifter pores. These conditions are suitable for capturing magnetically tagged or labeled cells at the sifter pores. Altering the external magnetic field can provide controlled capture and/or release of magnetically labeled cells from the sifter pores. The purpose of having a greater pore density at the periphery of the sifter than at the center is to provide improved flow rate uniformity through the sifter. Such flow rate uniformity is advantageous for cell quantification.

Abstract: A bonus game for a slot machine operable in a basic mode and a bonus mode. The bonus game is entered upon the occurrence of a special start-bonus game outcome in the basic mode. In the bonus game, a player selects, one at a time, from an array of windows each associated with a bonus game outcome. Credits are awarded based upon which ones of the windows are selected. The bonus game ends upon selection of a window associated with an end-bonus outcome but otherwise continues, allowing the player to make further selections and accumulate further credits until encountering an end-bonus outcome. In one embodiment, a bonus game resource obtained in the basic game may be exercised in the bonus game to affect the bonus game outcome. In one embodiment, for example, where the occurrence of an end-bonus outcome would otherwise end the bonus game, a player having a bonus game resource may exercise the bonus game resource upon encountering an end-bonus outcome to continue playing the bonus game.

Abstract: Method for securing a foundation form disposed on a moisture barrier membrane. The method can include penetrating the moisture barrier membrane with one end of a stake, sealing a breach in the moisture barrier membrane around a periphery of the stake caused by the penetrating step, and removing a portion of the stake extending above the moisture barrier membrane. A sealing member can be disposed on the stake at a location along an axial length of the stake.