Abstract: A system, configured to facilitate processing and detection of nucleic acids, the system comprising a process fluid container and a cartridge comprising: a top layer, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a detection chamber, comprises a portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

Abstract: A system, configured to facilitate processing and detection of nucleic acids, the system comprising a process fluid container and a cartridge comprising: a top layer, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a detection chamber, comprises a portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

Abstract: A method of preparing a slide of a biological specimen, including the steps of (a) providing a slide containing a biological specimen and a cover slip, (b) placing a liquid non-evaporating sealing compound such as mineral oil at spaced locations around an area on the slide and (c) placing the cover slip over the specimen area whereby the specimen and reagent is between the slide and the cover slip and the sealing compound spreads to define a closed boundary around the specimen in the space between the slide and the cover slip. Testing of the biological specimen may then be performed automatically.

Abstract: A method of preparing a slide of a biological specimen, including the steps of (a) providing a slide containing a biological specimen and a cover slip, (b) placing a liquid non-evaporating sealing compound such as mineral oil at spaced locations around an area on the slide and (c) placing the cover slip over the specimen area whereby the specimen and reagent is between the slide and the cover slip and the sealing compound spreads to define a closed boundary around the specimen in the space between the slide and the cover slip. Testing of the biological specimen may then be performed automatically.

Abstract: Methods and processing reagents for improving washing and aging a biological sample in an assay are disclosed. The processing reagents comprise an aqueous base reagent and a low vapor pressure composition in sufficient amount to raise the boiling point of the base reagent. The methods comprise applying the base reagent and low vapor pressure composition to the biological sample for use at an elevated temperature.

Abstract: A slide holder comprising a rectangular frame having two side walls facing each other, a first end wall facing a second end wall, the second end wall having a latch pivotally attached thereto, the latch capable of being pivoted in such a manner as to enable locking and unlocking of the slide holder in a slide holder carrier. The slide holder carrier has a first end wall, a first side wall, a second end wall, a second side wall, and a shaft running from the first end wall of the slide holder carrier to the second end wall of the slide holder carrier. At least one slide holder is rotatably mounted on the shaft.