Patents by Inventor Robert Jason Potter
Robert Jason Potter has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210054415Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.Type: ApplicationFiled: November 9, 2020Publication date: February 25, 2021Inventors: Xin YU, Xiquan LIANG, Xavier de MOLLERAT du JEU, Robert Jason POTTER
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Patent number: 10829787Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.Type: GrantFiled: October 14, 2016Date of Patent: November 10, 2020Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Xin Yu, Xiquan Liang, Xavier de Mollerat du Jeu, Robert Jason Potter
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Publication number: 20190390229Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents. Further disclosed are nucleic acid compositions with reduced cytotoxicity.Type: ApplicationFiled: April 20, 2017Publication date: December 26, 2019Inventors: Robert Jason POTTER, Jason CARTER
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Publication number: 20190119701Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.Type: ApplicationFiled: September 7, 2018Publication date: April 25, 2019Inventors: Xiquan LIANG, Robert Jason POTTER, Lansha PENG
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Publication number: 20180327738Abstract: The present disclosure generally relates to compositions and methods for genetic modification of cells. In particular, the disclosure relates to stabilized reagents for genome alteration (e.g., site specific nucleases), as well as stabilized reagents for non-genetic code altering modification (e.g., DNA methylation). Stabilization methods include storage of reagents at suitable temperatures, conversion to dry forms, and chemical modifications.Type: ApplicationFiled: November 18, 2016Publication date: November 15, 2018Inventors: Namritha RAVINDER, Robert Jason POTTER, Jason CARTE
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Patent number: 9862938Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.Type: GrantFiled: May 7, 2014Date of Patent: January 9, 2018Assignee: Life Technologies CorporationInventors: Michael D. Smith, Robert Jason Potter, Gulshan Dhariwal, Gary F. Gerard, Kim Rosenthal, Jun E. Lee
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Patent number: 9771565Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.Type: GrantFiled: August 23, 2010Date of Patent: September 26, 2017Assignee: Life Technologies CorporationInventors: Jun Lee, Robert Jason Potter
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Publication number: 20170107539Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.Type: ApplicationFiled: October 14, 2016Publication date: April 20, 2017Inventors: Xin YU, Xiquan LIANG, Xavier de MOLLERAT du JEU, Robert Jason POTTER
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Patent number: 9376698Abstract: An isolated mutant Tfi DNA polymerase having a D144A point mutation. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis.Type: GrantFiled: March 20, 2008Date of Patent: June 28, 2016Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Weidong Zheng, Jun E. Lee, Robert Jason Potter, David Mandelman
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Publication number: 20140363854Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.Type: ApplicationFiled: May 7, 2014Publication date: December 11, 2014Inventors: Michael D. SMITH, Robert Jason Potter, Gulshan Dhariwal, Gary F. Gerard, Kim Rosenthal, Jun E. Lee
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Publication number: 20120094332Abstract: The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may possess both a DNA-dependent DNA polymerase activity and an RNA-dependent DNA polymerase activity, i.e., a reverse transcriptase activity. The polypeptides of the present invention may be used in any application including, but not limited to, DNA sequencing reactions, amplification reactions, cDNA synthesis reactions, and combined cDNA synthesis and amplification reactions, e.g., RT-PCR.Type: ApplicationFiled: December 30, 2010Publication date: April 19, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jun Lee, Gary Gerard, Harini Shandilya, Katherine R. Griffiths, Moreland D. Gibbs, Peter L. Bergquist, Robert Jason Potter
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Publication number: 20120021464Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.Type: ApplicationFiled: December 28, 2010Publication date: January 26, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Michael D. SMITH, Robert Jason POTTER, Gulshan DHARIWAL, Gary F. GERARD, Kim ROSENTHAL, Jun E. LEE
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Publication number: 20110165651Abstract: The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.Type: ApplicationFiled: April 22, 2010Publication date: July 7, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Robert Jason Potter, Kim Rosenthal
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Publication number: 20110081704Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.Type: ApplicationFiled: August 23, 2010Publication date: April 7, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Michael D. Smith, Robert Jason Potter, Gulshan Dhariwal, Gary F. Gerard, Kim Rosenthal
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Publication number: 20100015683Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.Type: ApplicationFiled: September 2, 2009Publication date: January 21, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Michael D. Smith, Robert Jason Potter, Gulsham Dhariwal, Gary F. Gerard, Kim Rosenthal
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Publication number: 20080254525Abstract: A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5?-exonuclease activity and one of which has 5?-exonuclease activity.Type: ApplicationFiled: March 20, 2008Publication date: October 16, 2008Applicant: INVITROGEN CORPORATIONInventors: Weidong Zheng, Jun E. Lee, Robert Jason Potter, David Mandelman
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Patent number: 7078208Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.Type: GrantFiled: May 1, 2001Date of Patent: July 18, 2006Assignee: Invitrogen CorporationInventors: Michael D. Smith, Robert Jason Potter, Gulshan Dhariwal, Gary F. Gerard, Kim Rosenthal
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Patent number: 7056716Abstract: The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.Type: GrantFiled: March 15, 2001Date of Patent: June 6, 2006Assignee: Invitrogen CorporationInventors: Robert Jason Potter, Kim Rosenthal
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Publication number: 20040209276Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.Type: ApplicationFiled: September 15, 2003Publication date: October 21, 2004Inventors: Michael D. Smith, Robert Jason Potter, Gulshan Dhariwal, Gary F. Gerard, Kim Rosenthal, Jun E. Lee
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Publication number: 20030003452Abstract: The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.Type: ApplicationFiled: March 15, 2001Publication date: January 2, 2003Applicant: Invitrogen CorporationInventors: Robert Jason Potter, Kim Rosenthal