Abstract: Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.

Abstract: A grain cleaning module, adapted to be fitted to a grain cart (1) having means to deliver cleaned grain to collection means, includes a cleaning stage comprising at least one hollow cylinder (4) adapted to be mounted to said cart and means for causing the grain and waste material to pass through the one or more cylinders so that the grain can pass into the grain cart and the waste material can pass through the one or more cylinders for subsequent discharge. Each cylinder includes an outer sieve containing a rotary assembly carrying brushes arranged in a spiral to brush against the inside of the sieve and thereby to increase separation of grain and waste. The grain falls to the bottom of the cart where it can be moved by an auger to a chute and then out to another grain cart for example. A modular combine harvester includes a cutting head fitted to a tractor, a cart towed by the tractor and fitted with the module, and a conveyor module to carry the cut material from the header to the cart.