Abstract: Provided are compositions comprising an endonuclease and methods for purifying an endonuclease. One aspect of the invention provides a composition comprising at least 100 mg of an untagged endonuclease having an A260/A280 absorbance ratio of from about X to about 0.8, wherein X is less than 0.8. Another aspect of the invention provides a composition, generated by contacting a composition comprising at least 100 mg of an untagged endonuclease having an A260/A280 absorbance ratio of from about X to about 0.8, wherein X is less than 0.8, with an endonuclease binding molecule, wherein the endonuclease and the endonuclease binding molecule form a protein effector.

Abstract: One aspect of the invention provides a method of separating long polynucleotides from a composition. The method includes: introducing a composition including one or more long polynucleotides into a container including at least one boundary defined by a filter comprising a plurality of pores, wherein the pores have a smaller cross-sectional dimension than specified for the long polynucleotide's molecular weight; and applying elevated hydraulic pressure to the composition, thereby causing at least some of the one or more long polynucleotides to pass through the pores.

Abstract: Methods for treating the central nervous system (CNS) in a human patient with mucopolysaccharidosis (MPS) MB who has an intact blood brain barrier, comprising intravenous administration of recombinant NaGlu.

Abstract: Methods for treating the central nervous system (CNS) in a human patient with mucopolysaccharidosis (MPS) MB who has an intact blood brain barrier, comprising intravenous administration of recombinant NaGlu.

Abstract: A diagnostic test system for detecting the presence or absence of an analyte within a test sample is provided. For instance, the system may include a swab and a detection unit. The detection unit includes a first component that is capable of receiving the swab, the first component defining an insertion chamber within which a fluid is capable of being retained. The detection unit also includes a second component that defines a detection chamber within which an assay for detecting the presence or absence of the analyte is capable of being contained. The first component is rotatable relative to the second component from an inactive position to an active position. In the inactive position, the fluid remains substantially contained within the insertion chamber. In the active position, the fluid may flow from the insertion chamber to the detection chamber and contact the assay.

Abstract: A diagnostic test system for detecting the presence or absence of an analyte within a test sample is provided. For instance, the system may include a swab and a detection unit. The detection unit includes a first component that is capable of receiving the swab, the first component defining an insertion chamber within which a fluid is capable of being retained. The detection unit also includes a second component that defines a detection chamber within which an assay for detecting the presence or absence of the analyte is capable of being contained. The first component is rotatable relative to the second component from an inactive position to an active position. In the inactive position, the fluid remains substantially contained within the insertion chamber. In the active position, the fluid may flow from the insertion chamber to the detection chamber and contact the assay.

Abstract: Diagnostic devices for detecting the presence of an analyte in a sample are provided. Devices of the present invention comprise a means for inducing a pressure differential on a sample to direct the sample to a test surface. In one embodiment, the means for inducing a pressure differential on a sample to direct the sample to a test surface comprises a syringe that can be used to draw a sample from an opening to a test surface. In other embodiments, the device also provides means for diluting a sample.

Abstract: Diagnostic devices for detecting the presence of an analyte in a sample are provided. Devices of the present invention comprise a means for inducing a pressure differential on a sample to direct the sample to a test surface. In one embodiment, the means for inducing a pressure differential on a sample to direct the sample to a test surface comprises a syringe that can be used to draw a sample from an opening to a test surface. In other embodiments, the device also provides means for diluting a sample. In yet other embodiments, the device also provides a means.

Abstract: Diagnostic devices, systems and methods for detecting the presence of an analyte in a sample are provided. In one embodiment, the device comprises a substrate, a binder printed in a pattern onto a surface of the substrate, and a means for directing a sample towards the surface of the substrate that is printed with the binder. Systems of the present invention further include a light source that can be directed through the aforementioned means or another opening to the binder-printed surface to test for diffraction.

Abstract: A hydrophobic polymer fiber having a cationically charged coating thereon, in which the coating includes a functionalized cationic polymer, such as an epichlorohydrin-functionalized polyamine or an epichlorohydrin-functionalized polyamido-amine, which has been crosslinked by heat. The present invention also provides a fibrous filter which includes hydrophobic polymer fibers having the foregoing cationically charged coating thereon. The present invention further provides a method of preparing a fibrous filter.

Abstract: The present invention relates to a treatment for vaginal infections that does not promote colonization of artificially introduced bacteria such as Lactobacillus. Beneficial bacteria are contained within an outer cover of a vaginal insert that does not allow the bacteria to contact the vaginal tissue. The outer cover allows passage of metabolic byproducts produced by the beneficial bacteria into the vaginal tract creating an environment hostile to pathogenic microorganisms. The vaginal insert is placed in the vaginal tract by the user either by hand or by an instrument such as a tampon applicator. When the treatment time elapses the vaginal insert is removed.

Abstract: Diagnostic devices for detecting the presence of an analyte in a sample are provided. Devices of the present invention comprise a means for inducing a pressure differential on a sample to direct the sample to a test surface. In one embodiment, the means for inducing a pressure differential on a sample to direct the sample to a test surface comprises a syringe that can be used to draw a sample from an opening to a test surface. In other embodiments, the device also provides means for diluting a sample.

Abstract: Diagnostic devices, systems and methods for detecting the presence of an analyte in a sample are provided. In one embodiment, the device comprises a substrate, a binder printed in a pattern onto a surface of the substrate, and a means for directing a sample towards the surface of the substrate that is printed with the binder. Systems of the present invention further include a light source that can be directed through the aforementioned means or another opening to the binder-printed surface to test for diffraction.

Abstract: A hydrophobic polymer fiber having a cationically charged coating thereon, in which the coating includes a functionalized cationic polymer, such as an epichlorohydrin-functionalized polyamine or an epichlorohydrin-functionalized polyamido-amine, which has been crosslinked by heat. The present invention also provides a fibrous filter which includes hydrophobic polymer fibers having the foregoing cationically charged coating thereon. The present invention further provides a method of preparing a fibrous filter.

Abstract: A hydrophobic polymer fiber having a cationically charged coating thereon, in which the coating includes a functionalized cationic polymer, such as an epichlorohydrin-functionalized polyamine or an epichlorohydrin-functionalized polyamido-amine, which has been crosslinked by heat. The present invention also provides a fibrous filter which includes hydrophobic polymer fibers having the foregoing cationically charged coating thereon. The present invention further provides a method of preparing a fibrous filter.