Abstract: A cell preservation medium, a cell recovery medium and a cell culture medium, and methods which employ the media, are provided.

Abstract: A cell preservation medium, a cell recovery medium and a cell culture medium, and methods which employ the media, are provided.

Abstract: The present disclosure provides a method of fabricating cell, such as stem cell, arrays on a carrier where the surface energy of the carrier has been modified and patterned so that only areas of low contact angle are wetted by a water based cell solution. The patterned cell solution when applied to the carrier surface then self assembles into a 3 dimensional micro pattern on the carrier that mimics the surface topography of mammalian organs.

Abstract: The present invention discloses a novel combination of a natural product and minoxidil for the treatment of hair loss and regrowth. In the preferred embodiment, Sandalore® and its analogs are combined with minoxidil and formulated into a solution for the treatment and regrowth of mammalian hair loss. In another embodiment, Sandalore® and its analogs are combined with rosemary oil, emu oil or a combination thereof.

Abstract: The present subject matter discloses various exemplary projective capacitive micro structured electrode arrays for measuring the impedance and action potential of living cells in particular induced pluripotent stem cells that are subsequently differentiated into cardiomyocytes and neurons. In one exemplary system a projective capacitive electrode array with cardiomyocytes attached onto the electrodes is formed and electrical measurements of live cell responses when exposed to external stimulants, such as small molecule drugs or biologically active compounds is recorded.

Abstract: The present invention discloses a wearable, wireless, non-invasive blood glucose measurement system comprising an infrared LED-enabled wireless ring interfaced with machine learning software wherein the ring outputs data from the wearer used to determine the blood glucose concentrations of the wearer in real time. The blood glucose data analytics can be subsequently sent to, and displayed on, a smart mobile device, such as an iPhone®, or distributed over a cloud network.

Abstract: The present subject matter discloses various exemplary projective capacitive micro structured electrode arrays for measuring the impedance and action potential of living cells in particular induced pluripotent stem cells that are subsequently differentiated into cardiomyocytes and neurons. In one exemplary system a projective capacitive electrode array with cardiomyocytes attached onto the electrodes is formed and electrical measurements of live cell responses when exposed to external stimulants, such as small molecule drugs or biologically active compounds is recorded.

Abstract: The present disclosure provides a method of manufacturing and differentiating mammalian stem cells, and in one embodiment human induced pluripotent stem cells (iPSc), e.g., manufacturing neuron progenitors, e.g., derived from iPSC, on a large scale by the use of an automated hollow fiber reactor system. In one embodiment, human iPSc that can be differentiated into cardiomyocytes or neuron progenitors are provided. The method comprises seeding a hollow fiber reactor with stem cells such as iPSc, or differentiated iPSc, growing and expanding the seeded cells using appropriate growth factors and nutrients, and harvesting the cells after expansion from the hollow fiber reactor walls, e.g., with the use of an enzyme. The method can produce billions of cells per week from seeding the reactor with a minimum number of starting stem cells or neuron progenitor cells.

Abstract: The current disclosure describes a method of fabricating a n×n where n=1 to 100 micro well plate that has a transparent film bottom where the film has embossed on the surface micro structures for the facilitation of cell growth and differentiation in particular cardiomyocyte cells derived from human induced pluripotent stem cells. In one embodiment, the micro well plate has an array of 384 locations molded out of thermoplastic onto which a micro embossed film is laminated to the bottom. The micro embossed features are fabricated such that that when the film is laminated to the plate, e.g., with adhesive or via welding, the embossed microstructures are located within the individual microplate well locations.

Abstract: The present disclosure provides a fabrication process that results in creating large arrays of living cells, such as stem cells, which are subsequently exposed to nanoliter quantities of compounds to test the efficacy on cellular metabolism.

Abstract: The present disclosure provides a method of manufacturing and differentiating mammalian stem cells, and in one embodiment human induced pluripotent stem cells (iPSc), e.g., manufacturing neuron progenitors, e.g., derived from iPSC, on a large scale by the use of an automated hollow fiber reactor system. In one embodiment, human iPSc that can be differentiated into cardiomyocytes or neuron progenitors are provided. The method comprises seeding a hollow fiber reactor with stem cells such as iPSc, or differentiated iPSc, growing and expanding the seeded cells using appropriate growth factors and nutrients, and harvesting the cells after expansion from the hollow fiber reactor walls, e.g., with the use of an enzyme. The method can produce billions of cells per week from seeding the reactor with a minimum number of starting stem cells or neuron progenitor cells.

Abstract: The present disclosure provides a fabrication process that results in creating large arrays of living cells, such as stem cells, which are subsequently exposed to nanoliter quantities of compounds to test the efficacy on cellular metabolism.

Abstract: The present disclosure provides a fabrication process that results in creating large arrays of living cells, such as stem cells, which are subsequently exposed to nanoliter quantities of compounds to test the efficacy on cellular metabolism.

Abstract: The present disclosure provides a planar microelectronic human blood brain barrier stack used to model drug effects and transport across the brain capillary endothelial barrier to neurons. In one embodiment the stack is comprised of a carrier substrate, electrode arrays, astrocytes, extracellular matrix and brain capillary endothelial cells.

Abstract: The present disclosure provides an in vitro tubular blood brain barrier mimic used to model drug transport across the brain capillary endothelial barrier cells to neurons. In one embodiment the stack is comprised of interior neuro endothelial capillary cells, extracellular matrix, porous polymeric hollow fiber, exterior extracellular matrix, and neuron astrocytes. The tubular human blood brain barrier mimic is used to test molecular transport and effects of drug candidates across the multilayer stack.

Abstract: A cell preservation medium, a cell recovery medium and a cell culture medium, and methods which employ the media, are provided.

Abstract: The present invention discloses a high volume manufacturing process for the production and purification of exosomes. In one embodiment, a hollow fiber perfusion reactor is used to expand stem cells. The growth media used from the stem cell expansion is captured, filtered, centrifuged and exosomes isolated from the waste effluent. In one embodiment, stem cells are derived from bone marrow, fat, blood, cord blood and induced pluripotent stem cells are expanded and the exosomes captured from the growth waste effluent. The exosomes can be used as therapeutics and diagnostics for a number of regenerative and chronic diseases.

Abstract: The present disclosure provides a combination therapy to treat neurodegenerative diseases. In one embodiment, Alzheimer's disease is treated with an orally administered mitochondria stimulant, neuro protective antioxidant that crosses the blood brain barrier and is supplemented with a drug that promotes hippocampal neurogenesis. The combination therapy of a mitochondria stimulant, a brain available antioxidant and a neurogenesis agonist, may be employed to prevent, inhibit or treat neurodegenerative diseases that have a root cause of inflammation, oxidative stress, followed by free radical biologic macromolecule destruction, and eventually cell death, such as Parkinson's, age related Dementia, Multiple Sclerosis (MS) and Amyotrophic Lateral Sclerosis (ALS).

Abstract: The current disclosure describes a method of fabricating a n×n where n=1 to 100 micro well plate that has a transparent film bottom where the film has embossed on the surface micro structures for the facilitation of cell growth and differentiation in particular cardiomyocyte cells derived from human induced pluripotent stem cells. In one embodiment, the micro well plate has an array of 384 locations molded out of thermoplastic onto which a micro embossed film is laminated to the bottom. The micro embossed features are fabricated such that that when the film is laminated to the plate, e.g., with adhesive or via welding, the embossed microstructures are located within the individual microplate well locations.

Abstract: The present patent application discloses, among other things, methods and apparatus for a surface microstructured multielectrode array (MEA) for enabling the auto alignment of living cells on exposed electrodes. In one approach the microstructures are fabricated onto the surface of a multielectrode array and attached to a multi well plate such that the metal electrodes are exposed for the attachment of cells to the surface. The microstructure allows for the living cells to self organize into biologically relevant organ mimics onto the electrodes only, which allows electronic electrophysiology monitoting of their behavior when exposed to, e.g., therapeutic substances.