Abstract: A method for generating a series of ultra-thin sections of a microscopic sample is provided. The method includes detaching the sections from the sample using an ultramicrotome. The sections detached from the sample are made to float on a liquid surface and thereafter are transferred onto a solid carrier element. The method also includes determining, for at least one of the sections detached from the sample, a position and an orientation on the solid carrier element by monitoring the placement of the sections onto the solid carrier element using a monitoring system comprising a camera, and obtaining monitoring data.

Abstract: A method is proposed for generating a series of ultra-thin sections of a microscopic sample (10), wherein the sections (11) are detached from the sample (10) using an ultramicrotome (100) and wherein the sections (11), which are detached from the sample (10) are caused to float on a liquid surface and are thereafter transferred onto a solid carrier element (20). For at least for some of the sections (11) detached from the sample (10) a position and an orientation on the solid carrier element (20) are determined by monitoring the placement of these sections (11) onto the solid carrier element (20) using a monitoring system (400) comprising a camera (410), obtaining monitoring data. A method (2000) for the three-dimensional reconstruction of a microscopic sample (10), a microtome system and a computer program are also part of the present invention.

Abstract: Methods and apparatuses for capturing volume information of microscopic samples include a microscope system having at least one particle beam column, by which a beam of focused, charged particles can be generated, and an in-situ microtome, i.e., a microtome integrated in the microscope system. The method cam include a) providing a sample including a volume of interest (VOI); b) setting a cut surface lying within the sample; c) defining the set cut surface as processing surface; d) exposing the cut surface by virtue of ablating sample material by cutting with the in-situ microtome; and e) processing the sample with the particle beam, wherein the start point of the processing is disposed on the exposed processing surface.

Abstract: Methods and apparatuses for capturing volume information of microscopic samples include a microscope system having at least one particle beam column, by which a beam of focused, charged particles can be generated, and an in-situ microtome, i.e., a microtome integrated in the microscope system. The method cam include a) providing a sample including a volume of interest (VOI); b) setting a cut surface lying within the sample; c) defining the set cut surface as processing surface; d) exposing the cut surface by virtue of ablating sample material by cutting with the in-situ microtome; and e) processing the sample with the particle beam, wherein the start point of the processing is disposed on the exposed processing surface.