Patents by Inventor Robert L. Crombie

Robert L. Crombie has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9057079
    Abstract: The invention relates to improved genetic elements providing high levels of expression of operably-linked genes in a variety of tissues. In particular, fragments of unmethylated, CpG islands of less than 2 kb are shown to provide enhanced transgene expression and have advantages in terms of vector construction and cloning capacity.
    Type: Grant
    Filed: February 2, 2004
    Date of Patent: June 16, 2015
    Assignee: EMD Millipore Corporation
    Inventors: Steven G. Williams, Robert L. Crombie, Kai S. Lipinski, Alistair S. Irvine
  • Patent number: 8058028
    Abstract: The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) which is not derived from 13. The polynucleotide of any one of claims 1 to 7, wherein the UCOE comprises the sequence of FIG. 20 between nucleotides 1 to 7627 or a functional homologue or fragment thereof an LCR. The present invention also relates to a vector comprising the polynucleotide sequence, a host cell comprising the vector, use of the polynucleoticle, vector or host cell in therapy and in an assay, and a method of identifying UCOEs. The UCOE opens chromatin or maintains chromatin in an open state and facilitates reproducible expression of an operably-linked gene in cells of at least two different tissue types.
    Type: Grant
    Filed: September 22, 2008
    Date of Patent: November 15, 2011
    Assignee: Millipore Corporation
    Inventors: Michael Antoniou, Robert L Crombie
  • Publication number: 20100112640
    Abstract: The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) which is not derived from 13. The polynucleotide of any one of claims 1 to 7, wherein the UCOE comprises the sequence of FIG. 20 between nucleotides 1 to 7627 or a functional homologue or fragment thereof an LCR. The present invention also relates to a vector comprising the polynucleotide sequence, a host cell comprising the vector, use of the polynucleoticle, vector or host cell in therapy and in an assay, and a method of identifying UCOEs. The UCOE opens chromatin or maintains chromatin in an open state and facilitates reproducible expression of an operably-linked gene in cells of at least two different tissue types.
    Type: Application
    Filed: September 22, 2008
    Publication date: May 6, 2010
    Inventors: Michael Antoniou, Robert L. Crombie
  • Publication number: 20080222742
    Abstract: The invention relates to improved genetic elements providing high levels of expression of operably-linked genes in a variety of tissues. In particular, fragments of unmethylated, CpG islands of less than 2 kb are shown to provide enhanced transgene expression and have advantages in terms of vector construction and cloning capacity.
    Type: Application
    Filed: February 2, 2004
    Publication date: September 11, 2008
    Inventors: Steven G. Williams, Robert L. Crombie, Kai S. Lipinski, Alistair S. Irvine
  • Patent number: 6949361
    Abstract: The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) that does not occur in nature. The present invention also relates to a vector comprising the polynucleotide sequence, a host cell comprising the vector and use of the polynucleotide, vector or host cell in therapy, or for in vitro protein expression applications.
    Type: Grant
    Filed: September 20, 2001
    Date of Patent: September 27, 2005
    Assignee: M.L. Laboratories PLC
    Inventors: Michael Antoniou, Robert L. Crombie, Steven G. Williams
  • Publication number: 20020094967
    Abstract: The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) that does not occur in nature. The present invention also relates to a vector comprising the polynucleotide sequence, a host cell comprising the vector and use of the polynucleotide, vector or host cell in therapy, or for in vitro protein expression applications.
    Type: Application
    Filed: September 20, 2001
    Publication date: July 18, 2002
    Inventors: Michael Antoniou, Robert L. Crombie