Patents by Inventor Robert L. Erwin
Robert L. Erwin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 6846968Abstract: The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human ? galactosidase nucleotide sequences.Type: GrantFiled: July 26, 2000Date of Patent: January 25, 2005Assignee: Large Scale Biology CorporationInventors: Robert L. Erwin, Laurence K. Grill, Gregory P. Pogue, Thomas H. Turpen, Monto H. Kumagai
-
Publication number: 20040093646Abstract: The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human &agr; galactosidase nucleotide sequences.Type: ApplicationFiled: October 9, 2003Publication date: May 13, 2004Inventors: Robert L. Erwin, Laurence K. Grill, Gregory P. Pogue, Thomas H. Turpen, Monto H. Kumagai
-
Publication number: 20040064855Abstract: The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human &agr; galactosidase nucleotide sequences.Type: ApplicationFiled: October 9, 2003Publication date: April 1, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: Gregory P. Pogue, Thomas H. Turpen, Monto H. Kumagai, Robert L. Erwin, Laurence K. Grill
-
Publication number: 20040023281Abstract: The invention relates to &agr;-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human &agr; galactosidase nucleotide sequences.Type: ApplicationFiled: June 23, 2003Publication date: February 5, 2004Inventors: Thomas H. Turpen, Monto H. Kumagai, Gregory P. Pogue, Robert L. Erwin, Laurence K. Grill
-
Publication number: 20040016021Abstract: The invention relates to &agr;-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human &agr; galactosidase nucleotide sequences.Type: ApplicationFiled: June 23, 2003Publication date: January 22, 2004Inventors: Thomas H. Turpen, Gregory P. Pogue, Robert L. Erwin, Laurence K. Grill
-
Publication number: 20030167512Abstract: The present invention provides a method of compiling a plant positive sense functional gene profile, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, and a method of determining the presence of a trait in plant. The methods comprise expressing transiently a nucleic acid sequence of a plant into the cytoplasm of a host plant in a plus sense orientation to affect phenotypic or biochemical changes in the host plant. The nucleic acid sequence does not need to be isolated, identified or characterized prior to transfection into the host plant. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by systemically overproducing a new protein, or enhancing or suppressing the endogenous gene expression in a plus sense mechanism.Type: ApplicationFiled: September 6, 2002Publication date: September 4, 2003Inventors: Monto H. Kumagai, Guy R. Della-Cioppa, Robert L. Erwin, David R. McGee
-
Publication number: 20030077619Abstract: The present invention provides a method of compiling a functional gene profile of an organism, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, a method of determining the presence of a trait in plant, and a method of isolating human cDNA. The methods comprise expressing transiently a nucleic acid sequence of a non-plant donor organism into a host plant by a viral vector to affect phenotypic or biochemical changes in the host plant. The present invention provides a method for discovering the presence of a new gene and determining its function and sequence in a donor organism such as human by transfecting a nucleic acid sequence of the donor organism into a host plant to knock out the endogenous gene expression.Type: ApplicationFiled: May 8, 2002Publication date: April 24, 2003Inventors: Monto H. Kumagai, Guy R. della-Cioppa, Robert L. Erwin, David R. McGee
-
Publication number: 20030064392Abstract: The present invention provides a method of compiling a functional gene profile of an organism, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, a method of determining the presence of a trait in plant, and a method of humanizing plant cDNA. The methods comprise expressing transiently a nucleic acid sequence of a non-plant donor organism into a host plant by a viral vector to affect phenotypic or biochemical changes in the host plant. The present invention provides a method for discovering the presence of a new gene and determining its function and sequence in a donor organism such as human by transfecting a nucleic acid sequence of the donor organism into a host plant to knock out the endogenous gene expression.Type: ApplicationFiled: May 22, 2002Publication date: April 3, 2003Inventors: Monto H. Kumagai, Guy R. della-Cioppa, Robert L. Erwin, David R. McGee
-
Publication number: 20030041355Abstract: The present invention provides a method of compiling a positive sense functional gene profile of an organism, a method of changing the phenotype or biochemistry of an organism, a method of determining the presence of a trait in an organism, and a method of humanizing a plant cDNA. The methods comprise expressing transiently a nucleic acid sequence of a donor organism into a host plant to affect phenotypic or biochemical changes in the host organism. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced. The present invention provides a method for discovering new gene and its function in a donor organism such as human by transfecting a nucleic acid sequence of the donor organism into a host organism in a positive sense.Type: ApplicationFiled: May 14, 2002Publication date: February 27, 2003Inventors: Monto H. Kumagai, Guy R. della-Cioppa, Robert L. Erwin, David R. McGee
-
Publication number: 20030028926Abstract: The present invention provides a method of compiling a positive sense functional gene profile of an organism, a method of changing the phenotype or biochemistry of an organism, a method of determining a change in phenotype or biochemistry of an organism, and a method of determining the presence of a trait in an organism, and a method of isolating human cDNA. The methods comprise expressing transiently a nucleic acid sequence of a donor organism into a host plant to affect phenotypic or biochemical changes in the host organism. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by enhancing or supressing an endogenous gene expression in a positive sense mechanism, or by overexpressing a new protein. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced.Type: ApplicationFiled: May 1, 2002Publication date: February 6, 2003Inventors: Monto H. Kumagai, Guy R. della-Cioppa, Robert L. Erwin, David R. McGee
-
Publication number: 20030027182Abstract: The present invention provides a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, and a method of determining the presence of a trait in plant. The methods comprise expressing transiently a nucleic acid sequence of a plant into a host plant to affect phenotypic or biochemical changes in the host plant. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by systemically knocking out endogenous gene expression in an antisense mechanism. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced. The present invention exemplifies that genes encoding GTP binding proteins in one plant can silence endogenous gene expression in a different plant.Type: ApplicationFiled: March 21, 2002Publication date: February 6, 2003Inventors: Monto H. Kumagai, Guy R. Della-Cioppa, Robert L. Erwin, David R. McGee
-
Publication number: 20030027183Abstract: The present invention provides a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, and a method of determining the presence of a trait in plant. The methods comprise expressing transiently a nucleic acid sequence of a plant into a host plant to affect phenotypic or biochemical changes in the host plant. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by systemically knocking out endogenous gene expression in an antisense mechanism. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced. The present invention exemplifies that genes encoding GTP binding proteins in one plant can silence endogenous gene expression in a different plant.Type: ApplicationFiled: April 10, 2002Publication date: February 6, 2003Inventors: Monto H. Kumagai, Guy R. della-Cioppa, Robert L. Erwin, David R. McGee
-
Publication number: 20030027173Abstract: The present invention provides methods for rapidly determining the function of nucleic acid sequences by transfecting the same into a host organism to effect expression. Phenotypic and biochemical changes produced thereby are then analyzed to ascertain the function of the nucleic acids which have been transfected into the host organism. The invention also provides methods for silencing endogenous genes by transfecting hosts with nucleic acid sequences to effect expression of the same. The present invention also provides methods for selecting desired functions of RNAs and proteins by the use of virus vectors to express libraries of nucleic acid sequence variants. Moreover, the present invention provides methods for inhibiting an endogenous protease of a plant host.Type: ApplicationFiled: February 5, 2002Publication date: February 6, 2003Inventors: Guy Della-Cioppa, Robert L. Erwin, Wayne P. Fitzmaurice, Kathleen Hanley, Monto H. Kumagai, John A. Lindbo, David R. McGee, Hal S. Padgett, Gregory P. Pogue
-
Publication number: 20030024008Abstract: The present invention provides a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, a method of determining the presence of a trait in plant, and a method of increasing grain crop. The methods comprise expressing transiently a nucleic acid sequence of a plant into a host plant to affect phenotypic or biochemical changes in the host plant. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by systemically knocking out endogenous gene expression in an antisense mechanism. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced.Type: ApplicationFiled: April 24, 2002Publication date: January 30, 2003Inventors: Monto H. Kumagai, Guy R. Della-Cioppa, Robert L. Erwin, David R. McGee
-
Publication number: 20020192657Abstract: Contact lenses or similar ocular devices are disclosed that have specific binding molecule microarrays printed on or embedded in them to bind various analytes present in tears. Tear are used as a non-invasive alternative to the monitoring of proteins and other constituents found in serum, especially low molecular weight analytes and low abundance proteins. The devices can be placed in a developing reagent or the subject wearing the device can perceive changes in the visual filed that can be used to diagnose disease status as well as monitor various physiological and ambient environmental (exogenous) conditions.Type: ApplicationFiled: June 19, 2001Publication date: December 19, 2002Inventors: Robert L. Erwin, N. Leigh Anderson
-
Patent number: 6426185Abstract: The present invention provides a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, and a method of determining the presence of a trait in plant. The methods comprise expressing transiently a nucleic acid sequence of a plant into a host plant to affect phenotypic or biochemical changes in the host plant. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by systemically knocking out endogenous gene expression in an antisense mechanism. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced. The present invention exemplifies that genes encoding GTP binding proteins in one plant can silence endogenous gene expression in a different plant.Type: GrantFiled: July 21, 1999Date of Patent: July 30, 2002Assignee: Large Scale Biology CorporationInventors: Monto H. Kumagai, Guy R. della-Cioppa, Robert L. Erwin, David R. McGee
-
Patent number: 6386855Abstract: Laminae suitable for use in molds for forming retroreflective cube corner elements and methods for making such laminae are disclosed. A representative lamina includes a first row of cube corner elements disposed in a first orientation and a second row of optically opposing cube corner elements disposed. The working surface of a lamina is provided with a plurality of cube corner elements formed by the optical surfaces defined by three groove sets. Opposing first and second groove sets are formed in the working surface of a lamina. The first groove set forms a plurality of structures having first and second optical surfaces disposed in mutually perpendicular planes that intersect along a reference edge. The second groove set forms a corresponding plurality of structures on the opposite side of the lamina. A third groove is formed in the working surface of the lamina along an axis substantially perpendicular to the axes of the grooves of the first and second groove sets.Type: GrantFiled: September 27, 2001Date of Patent: May 14, 2002Assignee: 3M Innovative Properties CompanyInventors: Dan E. Luttrell, Robert L. Erwin, Kenneth L. Smith, Gerald M. Benson
-
Publication number: 20020028263Abstract: Laminae suitable for use in molds for forming retroreflective cube corner elements and methods for making such laminae are disclosed. A representative lamina includes a first row of cube corner elements disposed in a first orientation and a second row of optically opposing cube corner elements disposed. The working surface of a lamina is provided with a plurality of cube corner elements formed by the optical surfaces defined by three groove sets. Opposing first and second groove sets are formed in the working surface of a lamina. The first groove set forms a plurality of structures having first and second optical surfaces disposed in mutually perpendicular planes that intersect along a reference edge. The second groove set forms a corresponding plurality of structures on the opposite side of the lamina. A third groove is formed in the working surface of the lamina along an axis substantially perpendicular to the axes of the grooves of the first and second groove sets.Type: ApplicationFiled: September 27, 2001Publication date: March 7, 2002Applicant: 3M Innovative Properties CompanyInventors: Dan E. Luttrell, Robert L. Erwin, Kenneth L. Smith, Gerald M. Benson
-
Patent number: 6318987Abstract: Laminae suitable for use in molds for forming retroreflective cube corner elements and methods for making such laminae are disclosed. A representative lamina includes a first row of cube corner elements disposed in a first orientation and a second row of optically opposing cube corner elements disposed. The working surface of a lamina is provided with a plurality of cube corner elements formed by the optical surfaces defined by three groove sets. Opposing first and second groove sets are formed in the working surface of a lamina. The first groove set forms a plurality of structures having first and second optical surfaces disposed in mutually perpendicular planes that intersect along a reference edge. The second groove set forms a corresponding plurality of structures on the opposite side of the lamina. A third groove is formed in the working surface of the lamina along an axis substantially perpendicular to the axes of the grooves of the first and second groove sets.Type: GrantFiled: August 9, 2000Date of Patent: November 20, 2001Assignee: 3M Innovative Properties CompanyInventors: Dan E. Luttrell, Robert L. Erwin, Kenneth L. Smith, Gerald M. Benson
-
Patent number: 6257860Abstract: Laminae suitable for use in molds for forming retroreflective cube corner elements and methods for making such laminae are disclosed. A representative lamina includes a first row of cube corner elements disposed in a first orientation and a second row of optically opposing cube corner elements disposed. The working surface of a lamina is provided with a plurality of cube corner elements formed by the optical surfaces defined by three groove sets. Opposing first and second groove sets are formed in the working surface of a lamina. The first groove set forms a plurality of structures having first and second optical surfaces disposed in mutually perpendicular planes that intersect along a reference edge. The second groove set forms a corresponding plurality of structures on the opposite side of the lamina. A third groove is formed in the working surface of the lamina along an axis substantially perpendicular to the axes of the grooves of the first and second groove sets.Type: GrantFiled: August 10, 2000Date of Patent: July 10, 2001Assignee: 3M Innovative Properties CompanyInventors: Dan E. Luttrell, Robert L. Erwin, Kenneth L. Smith, Gerald M. Benson