Abstract: Apparatus and method are provided for assessing the direction of fixation of an eye by detecting polarization-related changes in light retroreflected from the fundus of the eye. Nerve fibers in the retina of the eye are birefringent and alter the polarization state of light traversing them as a function of their orientation. The nerve fibers are arrayed in a characteristic pattern in the retina, specifically radiating outward from the fovea and converging to the optic nerve head. By assessment of polarization-related changes in rctroreflected light from multiple retinal areas either sequentially or simultaneously, characteristic birefringence signatures of portions of the retina can be identified which are used to assess the direction of fixation of the eye. In addition, interference from the corneal birefringence is reduced by using incident light having a polarization state that is substantially independent of meridional direction.