Abstract: The present disclosure is notably directed to a microfluidic probe head (202), or MFP head, comprising a processing surface (204) having liquid injection and liquid aspiration apertures, as well as projections (205) extending from the processing surface (204). The arrangement of injection and aspiration apertures provides for a hydrodynamic flow confinement within a processing region that is formed between the processing surface (204) and a substrate (104) or sample surface (for example, the bottom of a microtiter plate sample well (102)), typically located beneath the processing surface (204). The disclosure is further directed to related microfluidic probe devices, and methods of operation of such an MFP head, notably to deposit cells on a surface.

Abstract: The present disclosure is notably directed to a microfluidic probe head, or MFP head, comprising a processing surface having a liquid injection aperture and a liquid aspiration aperture thereon. The aspiration aperture is generally shaped so as to partly extend around the injection aperture on the processing surface, although such injection apertures are not completely surrounded by the slit on the processing surface. Further, fluidic and solid barriers to aspiration are considered. The disclosure is further directed to related microfluidic probe devices, and methods of operation of such an MFP head, notably to deposit cells on a surface.

Abstract: If forms an object of the present invention a microfluidic chip (100, 200, 800, 900) for identifying and/or isolating small particles comprising at least a first microfluidic channel (110, 210), at least a first dielectrophoresis-producing electric field generating unit (DEP unit, 140, 240) positioned on a wall inside said channel (110, 210), at least one immunoaffinity capturing zone (150, 250) positioned inside said first channel (110, 210), on an opposing wall with respect to the one wherein said DEP unit (140, 240) is positioned and a voltage source (160, 260) electrically coupled to said DEP unit (140, 240), wherein said first channel (110, 210) includes a channel first end (112, 212) and a channel second end (114, 214) of a channel flow path for the fluid through the channel (110, 210). A further object is a method for the identification and/or isolation of small particles from a fluid sample comprising using the microfluidic chip according to the invention.

Abstract: The present disclosure is notably directed to a microfluidic probe head (202), or MFP head, comprising a processing surface (204) having liquid injection and liquid aspiration apertures, as well as projections (205) extending from the processing surface (204). The arrangement of injection and aspiration apertures provides for a hydrodynamic flow confinement within a processing region that is formed between the processing surface (204) and a substrate (104) or sample surface (for example, the bottom of a microtiter plate sample well (102)), typically located beneath the processing surface (204). The disclosure is further directed to related microfluidic probe devices, and methods of operation of such an MFP head, notably to deposit cells on a surface.

Abstract: The present disclosure is notably directed to a microfluidic probe head, or MFP head, comprising a processing surface having a liquid injection aperture and a liquid aspiration aperture thereon. The aspiration aperture is generally shaped so as to partly extend around the injection aperture on the processing surface, although such injection apertures are not completely surrounded by the slit on the processing surface. Further, fluidic and solid barriers to aspiration are considered. The disclosure is further directed to related microfluidic probe devices, and methods of operation of such an MFP head, notably to deposit cells on a surface.

Abstract: One or more embodiments of the present invention are directed to a method for processing a surface with a vertical microfluidic probe head. The method includes positioning the microfluidic probe head so as for the edge surface to face a surface to be processed. Next, the method dispenses processing liquid via each orifice of the first one of the sets of n orifices, so as for the dispensed processing liquid to process the surface; and aspirates liquid via each orifice of the second one of the sets of n orifices.

Abstract: One or more embodiments of the present invention are directed to a method for processing a surface with a vertical microfluidic probe head. The method includes positioning the microfluidic probe head so as for the edge surface to face a surface to be processed. Next, the method dispenses processing liquid via each orifice of the first one of the sets of n orifices, so as for the dispensed processing liquid to process the surface; and aspirates liquid via each orifice of the second one of the sets of n orifices.

Abstract: A microorganism culture device and a method of using the device. The device includes an open chamber, wherein microorganisms are likely to be deposited within a liquid for subsequent study. The open chamber simplifies the deposition of the microorganisms. The chamber is further provided with retention features, whereby microorganisms can be retained therein. In addition, the device includes an overflow area, wherein capillary structures are configured to retain excess liquid overflowing from the open chamber, e.g. when covering the device with a cover. As such, it allows for confining microorganism in the chamber, while excess fluid is captured externally, e.g. to seal the device with a cover.

Abstract: One or more embodiments of the present invention are directed to a vertical microfluidic probe head, which comprises a middle layer of material and two outer layers. The middle layer comprises two opposite, main surfaces, which are, each, grooved up to a same edge surface that adjoins each of the main surfaces, so as to form two sets of n microchannel cavities, n?1, on each of the opposite main surfaces. The middle layer is furthermore arranged between the two outer layers, which (at least partly) close the microchannel cavities grooved on the two main surfaces. This way, two sets of n microchannels are formed, which are, each, open on the edge surface, such that two opposite sets of n orifices are formed on the edge surface. Thus, the length of the apertures is not limited by the thickness of the middle layer.

Abstract: Disclosed herein is a microfluidic device comprising a chip; a flow channel being disposed in the chip; the flow channel being in communication with an entry port and an exit port; the flow channel being operative to permit the flow of a library from the entry port to the exit port; a substrate; the substrate being disposed upon the chip; the substrate being operative to act as an upper wall for the flow channels; and a plurality of receptors; the plurality of receptors being disposed on the substrate; the plurality of receptors being operative to interact with an element from the library.

Abstract: One or more embodiments of the present invention are directed to a vertical microfluidic probe head, which comprises a middle layer of material and two outer layers. The middle layer comprises two opposite, main surfaces, which are, each, grooved up to a same edge surface that adjoins each of the main surfaces, so as to form two sets of n microchannel cavities, n?1, on each of the opposite main surfaces. The middle layer is furthermore arranged between the two outer layers, which (at least partly) close the microchannel cavities grooved on the two main surfaces. This way, two sets of n microchannels are formed, which are, each, open on the edge surface, such that two opposite sets of n orifices are formed on the edge surface. Thus, the length of the apertures is not limited by the thickness of the middle layer.

Abstract: The invention is directed to a microfluidic probe head (100) with a base layer (120) comprising: at least two processing liquid microchannel (123, 124) in fluid communication with a processing liquid aperture (121, 122) on a face of the base layer; and an immersion liquid microchannel (223, 224) in fluid communication with a immersion liquid aperture (221, 222) on a face of the base layer, wherein the microfluidic probe head is configured to allow, in operation, processing liquid provided through the processing liquid aperture to merge into immersion liquid provided through the immersion liquid aperture. An additional layer can be provided to close the microchannels. Such a multilayered head is compact and easier to fabricate than heads made with unitary construction. The head can further be interfaced with tubing using e.g. a standard fitting for tubing port.

Abstract: The present invention is notably directed to a microfluidic surface processing device including a microfluidic probe head with at least one aperture, on a face, including at least an outlet aperture; and a surface processing structure extending outward and perpendicular with respect to the face, the processing structure being further dimensioned and located with respect to the outlet aperture such that it can intercept a flowpath of liquid dispensed via the outlet aperture. The present invention is further directed to related apparatuses and methods.

Abstract: The present invention is notably directed to a microfluidic surface processing device including a microfluidic probe head with at least one aperture, on a face, including at least an outlet aperture; and a surface processing structure extending outward and perpendicular with respect to the face, the processing structure being further dimensioned and located with respect to the outlet aperture such that it can intercept a flowpath of liquid dispensed via the outlet aperture. The present invention is further directed to related apparatuses and methods.

Abstract: The present invention is notably directed to a microfluidic surface processing device including a microfluidic probe head with at least one aperture, on a face, including at least an outlet aperture; and a surface processing structure extending outward and perpendicular with respect to the face, the processing structure being further dimensioned and located with respect to the outlet aperture such that it can intercept a flowpath of liquid dispensed via the outlet aperture. The present invention is further directed to related apparatuses and methods.

Abstract: The present invention is notably directed to a microfluidic surface processing device including a microfluidic probe head with at least one aperture, on a face, including at least an outlet aperture; and a surface processing structure extending outward and perpendicular with respect to the face, the processing structure being further dimensioned and located with respect to the outlet aperture such that it can intercept a flowpath of liquid dispensed via the outlet aperture. The present invention is further directed to related apparatuses and methods.

Abstract: A microfluidic surface processing system includes a microfluidic probe head having a processing fluid circuit configured to dispense a surface processing fluid from a processing fluid aperture thereof; a linkage mechanism, configured to apply a force to or modulate a force applied to the microfluidic probe head towards a surface to be processed; and a lifting fluid circuit integral with the microfluidic probe head and distinct from the processing fluid circuit, the lifting fluid circuit designed for dispensing a lifting fluid from a lifting fluid aperture thereof, with pressure such as to counter the force applied or modulated by the linkage mechanism, at the level of the surface.

Abstract: A microfluidic surface processing system includes a microfluidic probe head having a processing fluid circuit configured to dispense a surface processing fluid from a processing fluid aperture thereof; a linkage mechanism, configured to apply a force to or modulate a force applied to the microfluidic probe head towards a surface to be processed; and a lifting fluid circuit integral with the microfluidic probe head and distinct from the processing fluid circuit, the lifting fluid circuit designed for dispensing a lifting fluid from a lifting fluid aperture thereof, with pressure such as to counter the force applied or modulated by the linkage mechanism, at the level of the surface.

Abstract: A microfluidic device with interconnects. The device includes a first layer; a second layer, the first layer and the second layer assembled such as to face each other; a microchannel in said second layer; a tapered conduit having a tapered portion, wherein the tapered portion is inserted in a correspondingly shaped via formed in the first layer at the level of an end of the microchannel such that fluid communication is enabled between the microchannel and the conduit, and blocked in the via by way of the assembled first layer and second layer.

Abstract: The present invention is notably directed to a microfluidic surface processing device including a microfluidic probe head with at least one aperture, on a face, including at least an outlet aperture; and a surface processing structure extending outward and perpendicular with respect to the face, the processing structure being further dimensioned and located with respect to the outlet aperture such that it can intercept a flowpath of liquid dispensed via the outlet aperture. The present invention is further directed to related apparatuses and methods.