Patents by Inventor Robert M. Glaeser
Robert M. Glaeser has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240412942Abstract: This disclosure provides systems, methods, and apparatus related to electron microscopy sample preparation. In one aspect, a method includes providing an electron microscopy grid. An aqueous suspension including an electron microscopy sample is deposited on the electron microscopy grid. An edge of the electron microscopy grid is contacted with an absorbent material. A thickness of the aqueous suspension is allowed to decrease over a period of time. The aqueous suspension is then frozen.Type: ApplicationFiled: September 28, 2022Publication date: December 12, 2024Inventors: Maxim Armstrong, Robert M. Glaeser, Daniel A. Fletcher, Bong-Gyoon Han
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Publication number: 20240282549Abstract: A ponderomotive phase plate, also called a laser phase plate or standing wave optical phase plate, has a first mirror and a second mirror that define an optical cavity. An electron beam passes through a focal spot of the optical cavity. A laser with variable polarization angle of laser light is coupled to the optical cavity. A standing wave of polarized laser light, with an anti-node at the focal spot of the optical cavity, causes variable modulation of the electron beam. The variable modulation of the electron beam is controllable by the variable polarization angle of the laser light. In a transmission electron microscope, an image plane receives the electron beam modulated by the standing wave optical phase plate. An image formed at the image plane is based on the variable polarization angle of the polarized laser light.Type: ApplicationFiled: April 12, 2024Publication date: August 22, 2024Inventors: Jeremy J. Axelrod, Carter Turnbaugh, Sara Campbell, Osip Schwartz, Robert M. Glaeser, Holger Mueller
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Patent number: 11990313Abstract: A ponderomotive phase plate, also called a laser phase plate or standing wave optical phase plate, has a first minor and a second minor that define an optical cavity. An electron beam passes through a focal spot of the optical cavity. A laser with variable polarization angle of laser light is coupled to the optical cavity. A standing wave of polarized laser light, with an anti-node at the focal spot of the optical cavity, causes variable modulation of the electron beam. The variable modulation of the electron beam is controllable by the variable polarization angle of the laser light. In a transmission electron microscope, an image plane receives the electron beam modulated by the standing wave optical phase plate. An image formed at the image plane is based on the variable polarization angle of the polarized laser light.Type: GrantFiled: September 10, 2020Date of Patent: May 21, 2024Assignee: The Regents of the University of CaliforniaInventors: Jeremy J. Axelrod, Carter Turnbaugh, Sara Campbell, Osip Schwartz, Robert M. Glaeser, Holger Mueller
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Patent number: 11708637Abstract: This disclosure provides systems, methods, and apparatus related to graphene. In one aspect, a method includes submerging a frame support in an etching solution that is contained in a container. A growth substrate, a graphene sheet disposed on the growth substrate, and a primary support disposed on the graphene sheet is placed on a surface of the etching solution. The growth substrate is dissolved in the etching solution to leave the graphene sheet and the primary support floating on a surface of the etching solution. The etching solution in the container is replaced with a washing solution. The washing solution is removed from the container so that the graphene sheet becomes disposed on the frame support.Type: GrantFiled: August 6, 2020Date of Patent: July 25, 2023Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Bong-Gyoon Han, Robert M. Glaeser
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Publication number: 20230012465Abstract: This disclosure provides systems, methods, and apparatus related to graphene. In one aspect, a method includes submerging a frame support in an etching solution that is contained in a container. A growth substrate, a graphene sheet disposed on the growth substrate, and a primary support disposed on the graphene sheet is placed on a surface of the etching solution. The growth substrate is dissolved in the etching solution to leave the graphene sheet and the primary support floating on a surface of the etching solution. The etching solution in the container is replaced with a washing solution. The washing solution is removed from the container so that the graphene sheet becomes disposed on the frame support.Type: ApplicationFiled: August 6, 2020Publication date: January 12, 2023Applicant: The Regents of the University of CaliforniaInventors: Bong-Gyoon Han, Robert M. Glaeser
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Publication number: 20220319803Abstract: A ponderomotive phase plate, also called a laser phase plate or standing wave optical phase plate, has a first minor and a second minor that define an optical cavity. An electron beam passes through a focal spot of the optical cavity. A laser with variable polarization angle of laser light is coupled to the optical cavity. A standing wave of polarized laser light, with an anti-node at the focal spot of the optical cavity, causes variable modulation of the electron beam. The variable modulation of the electron beam is controllable by the variable polarization angle of the laser light. In a transmission electron microscope, an image plane receives the electron beam modulated by the standing wave optical phase plate. An image formed at the image plane is based on the variable polarization angle of the polarized laser light.Type: ApplicationFiled: September 10, 2020Publication date: October 6, 2022Inventors: Jeremy J. Axelrod, Carter Turnbaugh, Sara Campbell, Osip Schwartz, Robert M. Glaeser, Holger Mueller
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Publication number: 20210047740Abstract: This disclosure provides systems, methods, and apparatus related to graphene. In one aspect, a method includes submerging a frame support in an etching solution that is contained in a container. A growth substrate, a graphene sheet disposed on the growth substrate, and a primary support disposed on the graphene sheet is placed on a surface of the etching solution. The growth substrate is dissolved in the etching solution to leave the graphene sheet and the primary support floating on a surface of the etching solution. The etching solution in the container is replaced with a washing solution. The washing solution is removed from the container so that the graphene sheet becomes disposed on the frame support.Type: ApplicationFiled: August 6, 2020Publication date: February 18, 2021Applicant: The Regents of the University of CaliforniaInventors: Bong-Gyoon Han, Robert M. Glaeser
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Patent number: 10559449Abstract: This disclosure provides systems, methods, and apparatus related to arrangements including electron microscopy grids. In one aspect an arrangement includes an electron microscopy grid. The electron microscopy grid comprises a first surface and a second surface, with the first surface having a holey carbon film disposed thereon. A plurality of lipid molecules are disposed in a hole in the holey carbon film. Each lipid molecule of the plurality of lipid molecules has a hydrophilic head and a hydrophobic tail. A biotin-binding protein is attached to the hydrophilic heads of the plurality of lipid molecules. The biotin-binding protein is crystalline.Type: GrantFiled: December 18, 2017Date of Patent: February 11, 2020Assignee: The Regents of the University of CaliforniaInventors: Robert M. Glaeser, Bong-Gyoon Han
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Patent number: 10395888Abstract: An optical cavity is defined by a first mirror and a second mirror. The first mirror has a front surface that includes a first concave mirror. The second mirror has a front surface that includes a second concave mirror. The optical cavity has a resonant optical mode with a small focal spot size. The optical cavity may be used in a method of enhancing phase contrast in an electron beam image, and associated system for electron beam imaging or electron-beam spectroscopy, with a transmission electron beam microscope.Type: GrantFiled: March 28, 2018Date of Patent: August 27, 2019Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Jeremy J. Axelrod, Osip Schwartz, Robert M. Glaeser, Holger Mueller
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Patent number: 10309881Abstract: This disclosure provides systems, methods, and apparatus related to the preparation of electron microscopy specimens. In one aspect, a method includes providing an electron microscopy grid. An aqueous suspension including an electron microscopy sample is deposited on the electron microscopy grid. A portion of the aqueous suspension is removed from the electron microscopy grid. A volatile surfactant is contacted to the aqueous suspension. A thickness of aqueous suspension is allowed to decrease over a period of time. The aqueous suspension is then frozen.Type: GrantFiled: June 28, 2016Date of Patent: June 4, 2019Assignee: The Regents of the University of CaliforniaInventors: Robert M. Glaeser, Bong-Gyoon Han
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Publication number: 20180286631Abstract: An optical cavity is defined by a first mirror and a second mirror. The first mirror has a front surface that includes a first concave mirror. The second mirror has a front surface that includes a second concave mirror. The optical cavity has a resonant optical mode with a small focal spot size. The optical cavity may be used in a method of enhancing phase contrast in an electron beam image, and associated system for electron beam imaging or electron-beam spectroscopy, with a transmission electron beam microscope.Type: ApplicationFiled: March 28, 2018Publication date: October 4, 2018Inventors: Jeremy J. Axelrod, Osip Schwartz, Robert M. Glaeser, Holger Mueller
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Publication number: 20180108510Abstract: This disclosure provides systems, methods, and apparatus related to arrangements including electron microscopy grids. In one aspect an arrangement includes an electron microscopy grid. The electron microscopy grid comprises a first surface and a second surface, with the first surface having a holey carbon film disposed thereon. A plurality of lipid molecules are disposed in a hole in the holey carbon film. Each lipid molecule of the plurality of lipid molecules has a hydrophilic head and a hydrophobic tail. A biotin-binding protein is attached to the hydrophilic heads of the plurality of lipid molecules. The biotin-binding protein is crystalline.Type: ApplicationFiled: December 18, 2017Publication date: April 19, 2018Inventors: Robert M. Glaeser, Bong-Gyoon Han
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Publication number: 20170004953Abstract: This disclosure provides systems, methods, and apparatus related to the preparation of electron microscopy specimens. In one aspect, a method includes providing an electron microscopy grid. An aqueous suspension including an electron microscopy sample is deposited on the electron microscopy grid. A portion of the aqueous suspension is removed from the electron microscopy grid. A volatile surfactant is contacted to the aqueous suspension. A thickness of aqueous suspension is allowed to decrease over a period of time. The aqueous suspension is then frozen.Type: ApplicationFiled: June 28, 2016Publication date: January 5, 2017Inventors: Robert M. Glaeser, Bong-Gyoon Han
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Publication number: 20120043208Abstract: A multi-channel gel electrophoresis apparatus for efficiently collecting molecules isolated by gel electrophoresis so they can be further analyzed, identified, or used as reagents or medications. The apparatus using a novel “tagless” strategy that combines multi-dimensional separation of endogenous complexes with mass spectrometric monitoring of their composition. In this procedure, putative protein complexes are identified based on the co-migration of collections of polypeptides through multiple orthogonal separation steps. A majority of E. coli proteins are shown to remain in stable complexes during fractionation of a crude extract through three chromatographic steps.Type: ApplicationFiled: July 5, 2011Publication date: February 23, 2012Applicant: The Regents of the University of CaliforniaInventors: Jian Jin, Mark D. Biggin, Robert A. Nordmeyer, Ming Dong, Earl W. Cornell, Megan Choi, Halina Ewa Witkowski, Bong-Gyoon Han, Robert M. Glaeser
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Patent number: 7737412Abstract: A microfabricated electron phase shift element is used for modifying the phase characteristics of an electron beam passing though its center aperture, while not affecting the more divergent portion of an incident beam to selectively provide a ninety-degree phase shift to the unscattered beam in the back focal plan of the objective lens, in order to realize Zernike-type, in-focus phase contrast in an electron microscope. One application of the element is to increase the contrast of an electron microscope for viewing weakly scattering samples while in focus. Typical weakly scattering samples include biological samples such as macromolecules, or perhaps cells. Preliminary experimental images demonstrate that these devices do apply a ninety degree phase shift as expected.Type: GrantFiled: July 12, 2005Date of Patent: June 15, 2010Assignee: The Regents of the University of CaliforniaInventors: Jian Jin, Robert M. Glaeser